FastQCFastQC Report
Wed 30 Aug 2023
SRR10346285_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10346285_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88672318
Total Bases7.5 Gbp
Sequences flagged as poor quality0
Sequence length35-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAAAAGTCGTA13939431.5720159700798617No Hit
GAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTT9449681.0656854600327468No Hit
GAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCC8114450.9151052079184396No Hit
GAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGAT8108000.9143778106714205No Hit
GATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAAC7967830.8985701715838759No Hit
GATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCG7766600.8758765052245505No Hit
GAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCA6836550.7709903331950789No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAAAAGTCGTAA6509510.7341084734020373No Hit
CCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAAAAGTCGTA6242330.7039773111604007No Hit
GCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGG5804190.6545661747559143No Hit
GCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA5504710.6207923875408332No Hit
GAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGC5355590.6039754142888201No Hit
CCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATT5306190.5984043408000229No Hit
CCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA4503090.5078349254386245No Hit
CCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGG4459880.5029619277574315No Hit
GAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA4308340.4858720395693276No Hit
CAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCC3687100.4158118433308578No Hit
GGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACG3668250.41368603897329037No Hit
GGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAA3573070.4029521366521624No Hit
CAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGAT3332190.37578695078209184No Hit
GAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCC3277960.3696711751687827No Hit
GAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC3263780.3680720289730105No Hit
GGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATG3066520.34582607843859453No Hit
GAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGT3026050.34126208361892607No Hit
GAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGTCGCT2964530.3343241799543348No Hit
GAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTA2921490.3294703539835285No Hit
CATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCG2738420.3088246773925545No Hit
GCGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGG2735380.30848184210093615No Hit
GAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCA2705460.3051076210729035No Hit
GGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGAT2619890.29545748426245044No Hit
CAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTT2479260.279597968782095No Hit
GCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAAT2413810.2722168602832735No Hit
GATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAG2365610.2667811165148519No Hit
GATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAG2357050.26581576450950567No Hit
CCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAG2340770.26397979130307614No Hit
GACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGT2296140.258946653452772No Hit
CAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCA2263030.2552126809180741No Hit
GCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC2248230.25354361436677453No Hit
GAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCC2177600.24557833257499823No Hit
GGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGC2147420.24217479010755083No Hit
GAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAAT2049530.23113526816790783No Hit
GAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTA2033330.22930831694283668No Hit
GAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAAAAAAAAAAAAAA2013050.22702124466848833No Hit
GAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCC1818130.20503918708880486No Hit
CAGTGACGAAAAATAACAATACAGGAAAAAAAAAAAAAA1780930.20084396575716No Hit
GAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAAAAAAAAAAAAA1774460.2001143130148013No Hit
GATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAAT1711100.1929689037789674No Hit
GGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA1705210.19230466040145697No Hit
CCGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGG1655670.18671779844528255No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATG1632100.18405969718757098No Hit
GAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCT1610690.18164518942653557No Hit
CCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTG1581190.1783183338006344No Hit
GGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACT1568670.1769063937180485No Hit
GGAGGAATTCCCAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAGTCCC1541990.17389756293503006No Hit
GCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATT1527710.17228713926255992No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1523380.17179882452153783No Hit
GCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAAA1510790.17037899020526337No Hit
GGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGA1500790.16925124253546636No Hit
CGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAA1425950.1608111789747055No Hit
CAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTA1399650.15784520260313936No Hit
GACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGG1391130.1568843615884723No Hit
CCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGT1388880.15663061836276795No Hit
GAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATT1383390.1560114848920494No Hit
CAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCC1383140.1559832912003045No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCG1366440.15409995259174344No Hit
GAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCAAAAAAAA1344280.15160086375547327No Hit
CAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC1342410.15138997494122125No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAAT1335530.1506140845444009No Hit
CCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG1324110.1493261967054927No Hit
CTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC1304330.14709551181463418No Hit
GCATAACCCAGAGGTCGATGGATCGAAACCATCCTCTGCTACCAAAAAAA1296730.14623842358558847No Hit
GAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAG1295230.1460692614351189No Hit
GATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA1291670.14566778326467117No Hit
GAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG1253170.14132595473595266No Hit
GCATAACCCAGAGGTCGATGGATCGTAACCATCCTCTGCTACCAAAAAAA1242180.14008656004684572No Hit
GCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCAT1242040.1400707715794686No Hit
CAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGC1232890.13903888246160429No Hit
GAGTGACGAAAAATAACAATACAGGAAAAAAAAAAAAAA1220670.13766077480911235No Hit
GTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC1194920.13475682455938504No Hit
GAACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCC1188630.13404747127508274No Hit
GAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTC1164010.13127095651204246No Hit
GGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG1163870.1312551680446653No Hit
GAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTAAA1156980.13047814990017517No Hit
GGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCA1119280.1262265411850404No Hit
GAAGTGGCGTTCAGCCACCCGAGATTGAGCAAAAAAAAAAAAAA1118060.12608895596932518No Hit
CCATAACCCAGAGGTCGATGGATCGAAACCATCCTCTGCTACCAAAAAAA1107980.12495218631816978No Hit
GACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCGTGATGGGGATC1100390.12409622583679386No Hit
GGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCG1042380.11755416160430135No Hit
GAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAAAAAAAAAAAA1039090.11718313262093814No Hit
CCATAACCCAGAGGTCGATGGATCGTAACCATCCTCTGCTACCAAAAAAA1038990.11717185514424017No Hit
CCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCAAA1037210.1169711160590163No Hit
GAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCG1023940.11547459490119567No Hit
GATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG1021280.11517461402102964No Hit
GGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTT997000.11243644267876249No Hit
GCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGA991310.111794754254648No Hit
GAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGG989800.11162446435650863No Hit
GGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGT986880.11129516203692792No Hit
GACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCG979600.11047416173331569No Hit
CAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTA960900.10836527359079527No Hit
GGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGG957170.10794462370996098No Hit
CAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGT956350.10785214840103763No Hit
CATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAAC954530.10764689832513458No Hit
CGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGA948490.10696573873257718No Hit
GGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAG947200.10682025928317336No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAAA942920.10633758328050023No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA938060.1057894979129789No Hit
GAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTAAAAAA934260.10536095379845602No Hit
GGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAAAAAAAAAAAAAA930540.10494143166529153No Hit
GGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACAC928650.10472828735569989No Hit
GGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCG928150.10467189997221003No Hit
GATCTAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCG922950.10408547118391559No Hit
GAGGCGCGGGTAACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTG909110.10252466840891651No Hit
GGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAG908740.10248294174513403No Hit
GGCCCTGGCGGACGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAG908720.10248068624979445No Hit
GGAACGAGGAATTCCCAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAG905740.10214461744419492No Hit
CCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC895800.1010236362604167No Hit
CCCCTGGCGGACGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGT894340.10085898510062634No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)