FastQCFastQC Report
Tue 18 Jul 2023
SRR10346287_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10346287_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73804988
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length35-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCG7937521.075472026362229No Hit
GAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGAT5541830.7508747240769147No Hit
GATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCG4975390.674126523806223No Hit
GAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCC4887890.6622709565375174No Hit
GCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCG4727030.6404756816707294No Hit
GAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCA4251930.5761033387065926No Hit
GCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAAAAGTCGTA4079470.5527363543504675No Hit
GATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAAC3586600.4859563150393033No Hit
GATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG3456680.4683531687587294No Hit
GAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTT3441120.4662449101678602No Hit
GAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGC3439050.465964441319332No Hit
CGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGC3126280.4235865467520976No Hit
GGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATG3070350.41600846815394105No Hit
GGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACG2889260.3914721861346282No Hit
GCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGG2456580.3328474221823598No Hit
GCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCG2410980.32666897798289735No Hit
GAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA2398120.324926548324891No Hit
CAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGAT2336140.31652874193272684No Hit
GAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCC2277530.3085875442456545No Hit
GAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGTCGCT2230800.30225599386317903No Hit
CAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCC2223370.30124928683681923No Hit
GAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTA2140640.2900400173495049No Hit
GAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC2098410.28431818185513424No Hit
GCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA2050190.2777847480985974No Hit
CCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGG1976860.26784910526643535No Hit
GAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCA1967590.2665930925969394No Hit
GCGGCCGGGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGAC1959580.26550780009611274No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAAAAGTCGTAA1883420.2551887143454315No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG1864540.25263062165933825No Hit
CCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA1856240.25150603642127817No Hit
CCGCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAAAAGTCGTA1855220.25136783437997445No Hit
GGGGCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCGG1839840.2492839643846294No Hit
GGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCG1808770.24507422181275879No Hit
CATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCG1800170.24390898891549173No Hit
GACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGT1748270.2368769438726824No Hit
CCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATT1657500.22457831711862075No Hit
GAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGT1648520.22336159718635817No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATG1627120.22046206416292624No Hit
GGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA1568590.2125317058516424No Hit
CCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCG1523940.2064819792396687No Hit
GCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAG1517830.2056541219138197No Hit
GGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGC1503720.2037423270091176No Hit
GATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAG1500610.20332094627533845No Hit
GGAGGAATTCCCAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAGTCCC1497830.20294427796668704No Hit
CCGGCCGGGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGAC1474620.19979950406603955No Hit
CAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCA1388170.18808620360455855No Hit
GAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTA1385090.18766888763670012No Hit
GAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAAAAAAAAAAAAA1369910.18561211608082642No Hit
GAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAAT1339830.18153651078433886No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1230860.16677192603838648No Hit
CGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGA1220490.16536687195179817No Hit
GATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAG1198260.1623548804045602No Hit
GCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCAT1197130.1622017742215472No Hit
GGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACT1169760.15849335277989612No Hit
GCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC1121680.15197888793098915No Hit
GAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCC1101900.14929885226727496No Hit
GCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAAT1029550.13949599178852248No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGG1029290.13946076381720976No Hit
GGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGAT1026950.13914371207539522No Hit
GGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGA1026400.1390691913668491No Hit
GAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAAAAAAAAAAAAAA1008280.13661407275074688No Hit
GATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAAT993090.1345559462728996No Hit
CAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTA982420.13311024452710432No Hit
GCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCG959900.13005896024263294No Hit
GAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCAAAAAAAA945120.12805638556570187No Hit
GCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCA930020.1260104533856167No Hit
GAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCGGGTG927130.12561888093525603No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTGACCCG909350.12320982966625507No Hit
GGCCGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTG903470.12241313554579808No Hit
GGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCA894650.12121809436511255No Hit
CGCCGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTG891260.12075877581607358No Hit
GAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCC891160.1207452265963379No Hit
CAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTT880540.11930629946041045No Hit
GAAGAATGTTTTCATTAATCAAAAAAAAAAAAAAN854630.11579569662690008No Hit
GAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTC849360.1150816527468306No Hit
CAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGC843320.11426327987479654No Hit
CAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC837490.11347336036420735No Hit
GCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAAA820260.11113882980375257No Hit
GGAACGAGGAATTCCCAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAG799460.10832059209873457No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGC798560.1081986491211136No Hit
GCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGA795870.10783417511022425No Hit
GGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAAAAAAAAAAAAAA795540.10778946268509657No Hit
GAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCG790060.1070469654435822No Hit
GAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAG787570.1067095898721642No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC785060.10636950445679905No Hit
CCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAG768990.10419214484527793No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGC762450.10330602587456554No Hit
GACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCGTGATGGGGATC759330.10288329021881286No Hit
GGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGG750180.10164353661299966No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC738990.10012737892457892No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)