FastQCFastQC Report
Tue 18 Jul 2023
SRR10346289_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10346289_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59484574
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28641604.814962615349653No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA11114601.8684844242139147No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAA8572521.4411332928096618No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8516981.431796418345368No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAA7050621.1852854489636255No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA5642700.9485988753991916No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5124380.8614636796423892No Hit
GGAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGA4567880.7679100131069275No Hit
CCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAA4517710.7594758936997683No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3356710.5642992416823898No Hit
CGAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGA3334100.5604982562369867No Hit
GGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAA3165090.5320858480048962No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA2996200.5036936130701717No Hit
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2684970.45137248524298085No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAAA2602150.43744954784411844No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAA2378080.3997809583371985No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2370320.3984764184408549No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAA2191500.3684148431490826No Hit
GGGGAGAGAAAAAAAAAAAAAANNNNNNNNNNNNN2128360.3578003265182667No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGCAAAAAAAAAAAAAA1954360.3285490453373676No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAA1761800.29617762749717264No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGCAAAAAAAAAAAAAA1620140.2723630499564475No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGAAAAAAAAAAAAAA1619800.2723058922805768No Hit
CCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAA1598000.2686410765923952No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGAAAAAAAAAAAAAA1530370.25727174241846296No Hit
CCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAA1146990.19282141954988197No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAA1091530.18349799395049884No Hit
CCGCGCGCGGGTCGGGGGGCGGGGCGGACTGCAAAAAAAAAAAAAA1047960.17617340589847713No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAA979450.16465613421052658No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAAAAAAAAAAAAAA952650.1601507644654226No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAA941200.1582258956750703No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCAAAAAAAAAAAAAA863560.14517377227917946No Hit
CCGCGCGCGGGTCGGGGGGCGGGGCGGACTGAAAAAAAAAAAAAA843750.14184349710565297No Hit
GGCGCGCGGGTCGGGGGGCGGGGCGGACTGCAAAAAAAAAAAAAA797570.1340801398359178No Hit
GGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAA795090.1336632250236843No Hit
GGGGAGAGCGACGAAAAAAAAAAAAAANNNNNNNN762980.12826518687012872No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCAAAAAAAAAAAAAA751440.12632518810675183No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTATAAAAAAAAAAAAAA747770.1257082214289708No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTAAAAAAAAAAAAAA746260.12545437410378024No Hit
GGCGCGCGGGTCGGGGGGCGGGGCGGACTGAAAAAAAAAAAAAA730550.12281335325693012No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTAAAAAAAAAAAAAA705110.118536614215309No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAAA693610.11660333988438752No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA681320.11453725801247228No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTATAAAAAAAAAAAAAA633740.10653854560679883No Hit
GCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCAAAAAAAAAAAAA632940.10640405695769126No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAAAAAAAA619620.10416482095005002No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGAAAAAAAAAA607490.1021256368079563No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGAGAAAAAAAAA602880.10135064596747385No Hit
ACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGG598290.1005790173432191No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)