FastQCFastQC Report
Tue 18 Jul 2023
SRR10346290_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10346290_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54932533
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35301706.4263739667712025No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11738402.1368757927110336No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6484281.1804079742690912No Hit
GGAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGA5367740.9771513722114362No Hit
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3851860.7011983226770191No Hit
CGAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGA3807240.6930756315205782No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3437740.6258113020202436No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2716920.49459215725588335No Hit
GGGGAGAGAAAAAAAAAAAAAANNNNNNNNNNNNN2000990.36426319536366547No Hit
CATTGATCATCGACACTTCGAACGCAAAAAAAAAAAAAA1516660.2760950418943907No Hit
GATTGATCATCGACACTTCGAACGCAAAAAAAAAAAAAA1209010.22008997837401745No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1018540.1854165363173768No Hit
GACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAA994670.18107120601920906No Hit
GCGGGGCCCGAAGCGTTTACTTTGAAAAAAAAAAAAAA881680.1605023383866169No Hit
GAGGCATGCTCATAAGGAAAAAAAAAAAAAANNNN860500.15664669968887107No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAA815070.14837655492784213No Hit
GATGCTCATAAGGAAAAAAAAAAAAAANNNNNNNN774830.14105120548509933No Hit
ACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGG744550.13553898925432767No Hit
GGGGAGAGCGACGAAAAAAAAAAAAAANNNNNNNN743620.13536969067128218No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA707460.1287870704960938No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAA668220.12164376254049673No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAAAAAAAAA655340.11929906818606018No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGA653020.11887673193588216No Hit
CACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAA621960.11322252334513684No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)