FastQCFastQC Report
Sat 30 Sept 2023
SRR1039722.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1039722.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26337437
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTCT2448680.929733595565886Illumina Paired End PCR Primer 2 (97% over 40bp)
GGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATC409290.1554023650820693No Hit
CTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTA387950.14729983027581614No Hit
CTGGAACGTACCTTGAGCATATATGCTGTGACCCGAAAGA384490.1459861109492165No Hit
CAGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTA373950.14198420294275407No Hit
CGACTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGT356950.13552951260975013No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGC330600.12552474259359406No Hit
CTTATTTATCATTGCAGTCCAGCACGGATACGACCTTAGA327080.1241882420069956No Hit
CGGTAATACGCTTACATACATAAAGGTATAGTACTAACCA308240.11703492636736065No Hit
GGAAGGTACAATGGTATGCGTAGAAGTGTTTGGCGTAAGC283330.10757690659117666No Hit
CGGTAATGTATAGGTATACAACTTAAGCGCCATCCATTTT270830.10283081075808553No Hit
CGGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTA265510.10081087237152195No Hit
GGGAAACTTCGGAAGGAACCAGCTACTAGATGGTTCGATT264850.10056027851153475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)