FastQCFastQC Report
Sat 30 Sept 2023
SRR1039729.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1039729.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27779072
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length38
%GC66

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTCT5754262.0714370876032144Illumina Paired End PCR Primer 2 (97% over 38bp)
TCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAG783540.28206125820185785Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)