FastQCFastQC Report
Sat 30 Sept 2023
SRR1039736.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1039736.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31499780
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC66

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTCT5913851.8774258105929629Illumina Paired End PCR Primer 2 (97% over 40bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAG1331820.4228029529095124Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)