FastQCFastQC Report
Sat 30 Sept 2023
SRR1039743.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1039743.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24655895
Total Bases986.2 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTCT11319814.591117053345661Illumina Paired End PCR Primer 2 (97% over 40bp)
ATTTTTTTGGAGAGTTCATATCGATAAAAAAGATTGCGAC779890.3163097506701744No Hit
GTCCTTTCGTACTAAAATATCACAATTTTTTAAAGATAGA749820.3041138843266488No Hit
AAAAAATTAAGTTACTTTAGGGATAACAGCGTAATTTTTT668630.27118463961661093No Hit
CTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAAC527330.21387582969508914No Hit
TTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACC484120.19635060905312907No Hit
CTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTGG478360.19401445374422627No Hit
TTTTTGGAGAGTTCATATCGATAAAAAAGATTGCGACCTC349290.14166591802893386No Hit
CCGGTTTGAACTCAGATCATGTAAGAATTTAAAAGTCGAA305940.12408391583432685No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAG297640.12071758092740094Illumina Paired End PCR Primer 2 (97% over 36bp)
CTGGCTTACACCGGTTTGAACTCAGATCATGTAAGAATTT271180.1099858674771287No Hit
GTTTGAACTCAGATCATGTAAGAATTTAAAAGTCGAACAG264080.10710623159289087No Hit
ATTTGATCCATTAATAATGATTAAAAAATTAAGTTACTTT260780.10576780928049864No Hit
TTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCA249900.10135507147479336No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)