FastQCFastQC Report
Tue 18 Jul 2023
SRR10398490.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10398490.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134995957
Total Bases6.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC2005810.14858296830326556Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCCACTCGGGCACCAAGGACAGATCGGAAGAGCACACGTCTGAACTCC1529190.11327672576149816Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)