FastQCFastQC Report
Tue 18 Jul 2023
SRR10398495.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10398495.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53153078
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAACCAGACGTACCAACTGGAGGCCCAGTTGGTGCTATGCGTTCGAAA6411141.2061653325137633No Hit
CCTGAACCAGACGTACCAACTGGAGGCCCAGTTGGTGCTATGCGTTCGAA3156390.5938301447001809No Hit
GTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGCGTTACTTACCACG3031750.570380891206338No Hit
ATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGCGTTACTTAC2431920.4575313587672195No Hit
CGAGTATCGATGAAGAACGCAGCTTGCTGCGTTACTTACCACGAATTGCA2428670.45691991722473724No Hit
CGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGCGTTACTTACCA1814160.34130855037219104No Hit
CCCTGAACCAGACGTACCAACTGGAGGCCCAGTTGGTGCTATGCGTTCGA1176550.2213512451715402No Hit
CACGAGTCAAAAAATTAAGTAGTTAATGAGGGTTCTCAGTTACTACTCAG1001710.18845757154458675No Hit
GCCAAATGTTTCTTAAATCATAAACCGGGTATATATCTATTTTAATAAAT970280.18254446148913522No Hit
TGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGCGTTACT751800.14144053896558917No Hit
ACCAGACGTACCAACTGGAGGCCCAGTTGGTGCTATGCGTTCGAAATTTC746630.1404678765733943No Hit
CAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTAAACTGAAAAGATATT709020.13339208690793034No Hit
CCTAAATCTACTTTACTACAACTTACTCCCCTTTGGGCAATTGATGGATG690830.12996989562862193No Hit
ATTTGATCATGGTTTAAGATGATTTAAAATGGTATTATCTAAATTTGATT601350.11313549894514105No Hit
CAAGAACTCGGCGGCGTCTACGCTACACGGGTTGGGGAAAGGGGGTCTCG586790.11039624083482051No Hit
CTTTGGGCAATTGATGGATGATTTGTACCACTTCTAGATAATCTTCAAAA552420.10393001135324657No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)