FastQCFastQC Report
Tue 18 Jul 2023
SRR10398499.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10398499.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59848989
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGCGTTACTTAC11235101.8772414016885064No Hit
CCTGAACCAGACGTACCAACTGGAGGCCCAGTTGGTGCTATGCGTTCGAA8685221.4511890919326975No Hit
CCCTGAACCAGACGTACCAACTGGAGGCCCAGTTGGTGCTATGCGTTCGA6548881.0942340229005372No Hit
CTGAACCAGACGTACCAACTGGAGGCCCAGTTGGTGCTATGCGTTCGAAA4669530.7802186934185305No Hit
CGAGTATCGATGAAGAACGCAGCTTGCTGCGTTACTTACCACGAATTGCA3634760.6073218713853296No Hit
CGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGCGTTACTTACCA2028800.33898651153488996No Hit
GTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGCGTTACTTACCACG1415150.23645345120199107No Hit
CGGGAGGCATCTCTATCTCGTGGCAACACGAGAGCTTATGCCCTATGTAT1119420.18704075352049807No Hit
TGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGCGTTACT927760.1550168207519763No Hit
ACCAGACGTACCAACTGGAGGCCCAGTTGGTGCTATGCGTTCGAAATTTC845680.14130230336890068No Hit
GATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGCGTTA760460.1270631321775544No Hit
GTGGCGGGCGCTTGTGAGTCAGCTTCTTGACGGTAGATAAGTGTGGATGG666500.11136361885745472No Hit
GTCAAGAACTCGGCGGCGTCTACGCTACACGGGTTGGGGAAAGGGGGTCT661330.11049977803300903No Hit
GGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGCGTTACTT628050.10493911601414019No Hit
GTCTGAAGGTATCGAGAAGGCTGCGTATGTGTGTCAACTCACAAGCGCGG611660.10220055680472731No Hit
CAACCCTGAACCAGACGTACCAACTGGAGGCCCAGTTGGTGCTATGCGTT603190.10078532821999717No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)