FastQCFastQC Report
Fri 29 Sept 2023
SRR10399043_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10399043_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59333430
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA27929454.707203005118699No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT12200772.0563062004000106No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC9599871.6179529819867149No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA9427101.5888344901011116No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT8888811.4981116042001954No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA8027651.35297251482006No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6158051.0378719045907172Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC5819210.9807641324629304No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT5221380.8800064314501959No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG4829670.8139879996824725No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC4515300.7610043781389345No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC4295110.7238937644427433No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG4212520.7099741241994606No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA4181270.7047072788476918No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA3588470.6047973292627782No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA3542930.5971220608685525No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGA3329820.5612047036552581No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA3295850.5554794320840714No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA3139470.5291232952485638No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAAT2784330.4692683365852943No Hit
TCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT2772770.46732002515276805No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGAT2408990.40600888908664134No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC2327980.3923555405443441No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA2319370.3909044193130247No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGCTGTAGGCACCATCAAT2299370.3875336382878927No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGGCTGTAGGCACCATCAA1949050.3284910378516799No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1892820.31901408699952116No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1843270.3106629770097566No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA1791680.3019680473554285No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC1572470.26502260192946875No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1497220.2523400383224095No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC1491640.25139959041639764No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATA1487460.25069509718214505No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG1439350.24258668342618991No Hit
ACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACCATCAATAGATCGG1435620.2419580327650028No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1382880.23306928320172962No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCCTGTAGGCACCATCAATAGA1330620.2242614323830596No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1237630.2085889860067082No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATAG1215890.20492494703238964No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA1199390.20214405268665575No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1155990.19482945786211922No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1153590.19442496413910337No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGGACTGTAGGCACCATCA1137640.19173676627156055No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATA1133590.19105418311397132No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATAGATCG1115340.18797834542853836No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAG1061870.17896656235784786No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATAG1056870.17812386710156483No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA1027550.17318230211872127No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGA990300.16690422245941283No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG984700.1659604037723759No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA957120.1613120967387188No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGAT947920.15976153746715807No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA935450.15765985549798825No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA930260.15678513782196646No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGTAGGCACCATCAAT924520.15581772366775357No Hit
TGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGAT922350.15545199392652675No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATAG913880.15402446816238335No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGAT890120.15001998030452646No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA887200.14952784627485718No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA872180.14699638972498302No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGCTGTAGGCACCATCAAT865080.14579976246106116No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATAG857560.1445323487956115No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT857110.14445650622254602No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGACTGTAGGCACCATCAA854990.14409920343388205No Hit
TCGGGAGACCGGGGTTCGATTCCCCGACGGGGCTGTAGGCACCATCAATA853990.14393066438262545No Hit
TTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGG852190.14362729409036357No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG825740.13916943618462646No Hit
AGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCA824090.13889134675005305No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGACTGTAGGCACCATCAATAGA803180.1353671951882775No Hit
ACTGACCCGGTGAGGCGGGGGGGCGAGCCCCCTGTAGGCACCATCAATAG791270.13335989508781138No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG778910.13127675241427977No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC774800.13058405691361513No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT769250.129648665179141No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGACTGTAGGCACCATCAA768160.12946495761327131No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCAT767780.12940091277379379No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT759280.12796833083811268No Hit
AGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAG757610.12768687062251416No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAA745120.1255818178723192No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGCTGTAGGCACCA741400.12495485260164464No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATAGA736960.12420653921406531No Hit
TCGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG713830.12030823095850011No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC700230.11801609986141033Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA698090.11765542629172121No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC692710.1167486861959607No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG685170.11547790174948591No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT683460.11518969997183712No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTGATCTGTAGGCACCATCAATA683210.11514756520902297No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTGTAGGCACCATCAATA680060.11461666719756466No Hit
CCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGAT668180.11261442326863626No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA667520.1125031874948069No Hit
TTTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCG658670.11101161689118597No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATA652950.11004757351799821No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT651210.10975431556881173No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC645750.10883409234895065No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC636800.10732566784020407No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC617240.10402904399762496No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA617090.10400376313993646No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGAT609390.10270601244526062No Hit
NCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA605650.10207567639356092No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGATC605580.10206387865997298No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGATC603830.10176893532027392No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG594790.10024534229691423No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA594420.1001829828479493No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)