FastQCFastQC Report
Fri 29 Sept 2023
SRR10399044_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10399044_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71230363
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA8544351.1995376185293343Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGA7156111.0046432025062122No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA6314350.8864688784472431No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAAT5703970.8007778929892579No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA5685110.7981301457076668No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT5671790.796260156641347No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGCTGTAGGCACCATCAAT5323560.7473722968392004No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG4746150.6663099554890658No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT4725360.6633912563382557No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC4648360.6525812594834032No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGGCTGTAGGCACCATCAA3782130.5309716026577037No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATAG2983630.4188705313771881No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATA2919340.409844885951234No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2814490.395125039584594No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2802990.3935105595348433No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGAT2750480.38613870323811206No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATAG2735170.38398933892840054No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGACTGTAGGCACCATCAA2533620.3556938211869003No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2528460.3549694110080557No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG2452290.3442759374959243No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2396110.33638885147896835No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC2341760.32875867837427697No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGGACTGTAGGCACCATCA2149330.30174351350701384No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGACTGTAGGCACCATCAA2100310.29486161680798956No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2031610.28521685338034847No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCCTGTAGGCACCATCAATAGA2001800.28103184031225564No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT1909450.26806686356490983No Hit
TTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCCTGTAGGCACCATCA1819860.2554893620295042No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC1740200.24430592891966588No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1721690.241707317987415No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC1644380.23085380036600403No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAG1619830.22740723643371016No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGCTGTAGGCACCATCAAT1552680.21798007683886153No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGA1499460.21050854394775442No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGTAGGCACCATCAAT1498660.2103962322921196No Hit
TTTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGTAGGCACCATCAA1497780.21027268947092126No Hit
TGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGAT1464730.2056328141975073No Hit
TTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGCTGTAGGCACCATC1395120.19586029626158158No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATAG1349660.18947818643013234No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTGATCTGTAGGCACCATCAATA1337420.18775981809891942No Hit
TCGGGAGACCGGGGTTCGATTCCCCGACGGGGCTGTAGGCACCATCAATA1334980.18741726754923319No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCCCTGTAGG1299840.18248397807547323No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTGTAGGCACCATCAATA1225570.17205724474547462No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATAGA1184210.16625073214915387No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCCTGTAGGCACCATCAA1147430.1610872037813425No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATA1143120.16048212473660986No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGAT1139070.15991354697995852No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATAG1129070.15850965128452313No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG1110070.15584224946319591No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1097370.15405930192999298Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1082150.1519225726815403No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA1067450.1498588460092503No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATAGATCG1049630.14735710387998444No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC1046240.14688118323923186No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGCTGTAGGCACCATCAATAGA1022210.1435076218831006No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGCTGTAGGCACCATCAATA1011510.14200545348898475No Hit
TCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT1000950.14052293963460497No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA996130.13984626190940513No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA994970.13968341000873463No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGCTGTAGGCACCATCAATA978800.1374133106692156No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG977150.13718166787946875No Hit
TTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGG961590.1349972061773713No Hit
TTTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCG957020.13435562584455732No Hit
TCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGCTGTAGGCACCATCAAT955230.1341043285150744No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGAT940050.13197321484940347No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA924890.12984490897512344No Hit
TCCCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAG902380.12668473976469838No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGCTGTAGGCACCATCAATA892850.12534682716694848No Hit
TCTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAAT876340.12302899537378464No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCCTGTAGGCACCATCAATA843810.11846212267653332No Hit
TTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATA834180.11711017112182903No Hit
TCGCATCGACCTGGTATTGCAGTACCTCCAGGAACGGTGCCTGTAGGCAC804570.11295323596764487No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCCCTGTAGGC784420.11012438614134257No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGACTGTAGGCACCATCAATAGA763640.10720709088622782No Hit
ACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACCATCAATAGATCGG762240.10701054548886689No Hit
TCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATA753350.10576248221562481No Hit
TCCTCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAA745880.10471377213113459No Hit
CCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG739250.10378298928506093No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCA737470.10353309585127342No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATA734670.1031400050565515No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGATC713140.10011741762427913No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)