FastQCFastQC Report
Fri 29 Sept 2023
SRR10399045_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10399045_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66450250
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT16834192.53335239521296No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA11470911.7262403075985417Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC11035611.6607326533760218No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT6666221.0031896042528057No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA6215310.9353328241804959No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG5673220.853754500547402No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGA4948170.7446427966787182No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA4167160.6271097550423061No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC3876380.5833507022170722No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGAT3413590.5137061184871389No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAG3349380.5040432504016162No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3098730.4663233020191798No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3065010.46124882901117753No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2820040.42438365544147694No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGA2794490.42053867366939923No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC2780440.418424309916065No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA2668650.40160119788864607No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAAT2623160.3947554749605908No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATAG2370710.35676464723609014No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGGCTGTAGGCACCATCAA2128020.32024258749967077No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2040740.30710794918002565No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATA2008870.30231187994025605No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCC1961720.2952163460634083No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG1907310.28702826550690175No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATAGATCG1901210.28611028551435097No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGCTGTAGGCACCATCAAT1816160.27331123660181866No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCCTGTAGGCACCATCAATAGA1812090.2726987483117069No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC1790960.2695189258129202No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC1743100.2623165450844805No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA1730170.26037072847731946No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGA1698510.2556062618274574No Hit
TGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGAT1570950.23640994578650945No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1542000.23205330303497732No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGAT1536430.23121508195981205No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC1440420.2167666788311556No Hit
ACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACCATCAATAGATCGG1417300.21328738417086468No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTGATCTGTAGGCACCATCAATA1388030.20888258509185442No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1382980.20812261804884105Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC1335850.2010300939424607No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1275660.1919721897208814No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTACTGTAGGCACCATCAATAGA1247840.18778559900075623No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGAT1225340.184399607224954No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1219680.18354784218268552No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATAG1216510.18307079356360587No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATAG1216200.18302414212136148No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGGACTGTAGGCACCATCA1195260.1798729124420149No Hit
TTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCCTGTAGGCACCATCA1167460.17568933149235705No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA1153450.17358098727995758No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATAG1137090.17111899503764094No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGACTGTAGGCACCATCAA1102190.1658669455720633No Hit
TTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGG1062260.15985793883393967No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGAT1028740.15481356353061124No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1005200.1512710636905053No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGACTGTAGGCACCATCAA979090.14734180834534105No Hit
TCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT967130.14554196560584798No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA949320.14286176500464634No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGATC946650.14245996064725114No Hit
ACTGACCCGGTGAGGCGGGGGGGCGAGCCCCCTGTAGGCACCATCAATAG936410.14091895816795272No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGCTGTAGGCACCATCAAT911460.13716426950989652No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG891240.1341213915673756No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTGTAGGCACCATCAATA888990.13378279238979537No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAGA853710.12847355728533755No Hit
TTTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCG850130.12793480837167656No Hit
TTCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGA843510.12693857434697386No Hit
TCCCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAG822240.12373768345491551No Hit
TGGGCCGCCGGTGAAATACCACTACTCTGATCTGTAGGCACCATCAATAG813130.12236673300702405No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC791030.11904093664056946No Hit
TCGGGAGACCGGGGTTCGATTCCCCGACGGGGCTGTAGGCACCATCAATA787360.11848864375980526No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATA772690.11628097712198222No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGATC756680.11387165586284477No Hit
TTCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGAT747070.1124254611532688No Hit
TTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGCTGTAGGCACCATC745650.11221176745008483No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGACTGTAGGCACCATCAATAGA723700.10890854436213558No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAAT721380.10855941098791955No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATAGA715570.10768507266714572No Hit
CCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGAT708810.10666777024917137No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC690840.10396349148423069No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGCTGTAGGCACCATCAATAGA664940.10006583872897393No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)