FastQCFastQC Report
Fri 29 Sept 2023
SRR10399046_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10399046_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50412459
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA32466496.440171863070596Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT5668811.1244859132937752No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC4771520.9464961826202527No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG4291650.8513074119237072No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3863520.766381977121965Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG3802090.7541964973380886No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGAT3671940.7283794666711259No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGGCTGTAGGCACCATCAA3104920.6159033027926687No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2924700.5801542035471827No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2768950.5492590631216778No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAAT2643730.5244199653105595No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCCTGTAGGCACCATCAATAGA2643680.5244100471274372No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA2321450.46049132417841393No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG2208260.4380385412264853No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGA2199930.4363861719183347No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATAG1757320.34858843128441724No Hit
GTGTAATGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA1677100.3326756982832359No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATA1424480.28256506987687313No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC1337870.2653847930726807No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC1309370.2597314286930538No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGCTGTAGGCACCATCAAT1291990.25628386823979366No Hit
TTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGG1182320.23452932537966456No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1142270.2265848606988205No Hit
TTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCCTGTAGGCACCATCA1119410.22205026737537242No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1051320.2085436855996253Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATAG1046850.20765700002850487No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1043710.2070341381284337No Hit
GTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATAGATCGGAAGA1027520.20382263043348076No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGCTGTAGGCACCATCAAT1012490.20084122458696174No Hit
GTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATAGATCGGAAG998440.19805421512963692No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA998060.19797883693790855No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA985350.1954576347882574No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC947720.1879932101705255Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATAG924010.18329000773400084No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGATC868020.17218362627381456No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGACTGTAGGCACCATCAA866270.17183648986453925No Hit
GTGTAATGGTTAGCACTCTGGCTGTAGGCACCATCAATAGATCGGAAGAG864000.17138620435079352No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCC857340.17006510235892283No Hit
TGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGAT818740.16240826498862118No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGAT780420.15480696944380357No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAGA778740.15447371849089925No Hit
TCGGGAGACCGGGGTTCGATTCCCCGACGGGGCTGTAGGCACCATCAATA762490.15125030897619973No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT740830.1469537520476833No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATAGATCGGAAGA739360.14666215746389202No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAG720460.1429130842437184No Hit
ATCGGAAGAGCGTCGTGTAGGCACCATCAATAGATCGGAAGAGCACACGT719500.14272265512777307Illumina Single End PCR Primer 1 (95% over 23bp)
TTTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCG714860.14180224773403732No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGAT710730.14098300580814754No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGA706110.14006656568766065No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGA700880.13902912373308352No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCCTGTAGGCACCATCAATAGAT695520.13796589450238878No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATA682990.13548039781197738No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATAG679320.1347524031708114No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGCTGTAGGCACCATCAATAGA668230.132552550154318No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGACTGTAGGCACCATCAATAGA659410.1308029826515703No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC642410.1274308003900385No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGACTGTAGGCACCATCAA641090.12716896035561367No Hit
TCGGGTCGGGGCGGCGGCGGCGGCTGTAGGCACCATCAATAGATCGGAAG637810.12651832754280048No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTGATCTGTAGGCACCATCAATA627670.12450692000562798No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGCTGTAGGCACCATCAATA626340.12424309633457871No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATAGATCG618760.12273949977326042No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTACTGTAGGCACCATCAATAGA618610.12270974522389395No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATA603680.11974817574361926No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG579850.11502116966760141No Hit
TTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGCTGTAGGCACCATC577990.11465221325545735No Hit
GTCTAGGGGTATGATTCTCGGTCTGTAGGCACCATCAATAGATCGGAAGA565430.11216076565517266No Hit
TTCGTGCCCCACGTTGGGCGCCACTGTAGGCACCATCAATAGATCGGAAG563260.11173031650767125No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAG561230.11132763827291187No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGACTGTAGGCACCATCAATAGA556500.11038937814955624No Hit
ACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACCATCAATAGATCGG556000.11029019631833473No Hit
NTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA552860.10966733441826355Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC546280.1083621015193883No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATA542760.10766386142758877No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGCTGTAGGCACCATCAATAGA541970.10750715413425876No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATC541320.10737821775367076No Hit
TTCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGA538530.10682478313545467No Hit
CCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGAT530530.1052378738359103No Hit
TCCCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAG514570.10207198978331923No Hit
TCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGCTGTAGGCACCATCAAT509160.10099884236950236No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCCTGTAGGCACCATCAATAGAT506720.10051483503314132No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG504880.10014984589424611No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)