FastQCFastQC Report
Fri 29 Sept 2023
SRR10399048_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10399048_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59619176
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA25707214.311902935391123No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT14535562.4380679129144625No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC10647401.7859019051185814No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA8800921.4761894729977483No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7864831.319177910140858Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT7762151.3019552635212535No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA6858721.150421803884039No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA5963061.0001916162008009No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC5649250.9475558669244272No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC5139650.8620800126455959No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA4531440.7600641780087668No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3944410.6616008916325848No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT3934310.6599068058236833No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC3930840.6593247783230013No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3694700.6197167166483482No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA3534490.5928444901687336No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG3351960.562228501782715No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA3341360.5604505503397095No Hit
TCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCAT3002810.5036651294878681No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAAT2814460.47207294512087855No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGA2702300.45326020607866163No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC2596980.435594748910988No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2284310.3831502132803714No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGAT2119030.3554275892709419No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGCTGTAGGCACCATCAAT2117480.35516760580521944No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA2065450.34644054792035367No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGGCTGTAGGCACCATCAA2027350.3400499866016263No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1923600.32264786752503927No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG1917410.3216096109748313No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1637820.2747136256965376No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA1628150.2730916643329656No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA1582140.2653743486827124No Hit
TTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGG1555530.26091101963569574No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA1544530.25906597568540696No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC1531030.2568016035645981No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCCTGTAGGCACCATCAATAGA1468710.24634859092987127No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATA1391280.2333611588325206No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA1363660.22872842120461376No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC1359890.22809607432346937No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1303460.21863099885848805No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1277040.21419953875243095No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCAT1276510.21411064118028064No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG1269120.21287110710822305No Hit
TTTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCG1238820.20778884968151856No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1191430.19984006488113823No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGCTGTAGGCACCA1163780.19520229531518515No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGAT1136710.19066180988479278No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGAT1037590.1740362865800091No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC1013300.16996209407523513No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATAG968210.16239909119173335No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC951690.15962817064093607Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG951430.15958456051120198No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATA949180.1592071651577338No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC930370.15605214000274006No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATAG883680.14822076709010537No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA843210.14143268266572487No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGATC829540.13913979622932057No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATAG806640.13529875018735582No Hit
TCGGGAGACCGGGGTTCGATTCCCCGACGGGGCTGTAGGCACCATCAATA803620.13479220175736745No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA803590.13478716981932123No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCAT799830.1341565002508589No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGA799110.13403573373774907No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC797180.1337120123901075No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA784420.13157176140777258No Hit
TGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGAT769330.12904069657051281No Hit
GTGTAATGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA750340.1258554797872416No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA741290.1243375118099586No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAG738470.12386450963361184No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT726520.12186012097852544No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA719910.12075141729567011No Hit
GTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATAGATCGGAAGA694170.11643401445199444No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATAG685170.11492443303812183No Hit
AGCGCCGCGGAGCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCA682550.11448497711541669No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGACTGTAGGCACCATCAA676090.11340143312279258No Hit
GTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATAGATCGGAAG671650.11265670629194875No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG670490.11246213802082738No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC663810.11134169314919751No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG663570.11130143764482756No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGTAGGCACCATCAAT655330.10991933199479308No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATA652240.10940104237603014No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA651370.10925511617268914No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGCTGTAGGCACCATCAAT648470.10876869549488574No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGACTGTAGGCACCATCAATAGA646550.10844665145992625No Hit
TCGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG644020.10802229135135984No Hit
TCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGCTGTAGGCACCATCAAT641860.10765999181203041No Hit
CCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGAT639380.10724401826687441No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGATC637850.10698738942651606No Hit
TCGGGTCGGGGCGGCGGCGGCGGCTGTAGGCACCATCAATAGATCGGAAG635070.10652109650089764No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA634260.1063852341736491No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT632960.10616718352497861No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA628200.1053687826883082No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGCTGTAGGCACCATCAATA617520.10357741274384603No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCAC613420.10288971454419295No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAA605990.10164347122140702No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGACTGTAGGCACCATCAA605790.10160992496776541No Hit
TTCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGA602030.10097925539930307No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)