FastQCFastQC Report
Tue 18 Jul 2023
SRR10400973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10400973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3928241
Total Bases200.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA1580084.022360135236102No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT1576514.01327209812229No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT714501.8188802570921692No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA594961.514571025555713No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA491801.2519598466591026No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC482821.229099742098308No Hit
TTAACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATC454291.156471815247588No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC393581.0019242709395886No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA390420.9938799579761017No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC356480.9074799636783994No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT327290.8331718955125208No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC326370.830829880345936No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA280500.7140600589424121No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT256950.6541095620151616No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC253920.6463961859773879No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT239480.6096367305366448No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT227610.579419643550383No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA215490.5485661393992883No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA210310.5353795757439526No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT208820.531586529441549No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCACTGTAGGCACCAT205280.5225748623875165No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC198800.5060789294750501No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT190390.4846698560500743No Hit
GGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCTC158700.4039976162358674No Hit
GTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCG143940.3664235468241383No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC142090.36171405980437554No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCAA136250.3468473548338811No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT134000.3411196003503858No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC131620.3350609089411775No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA131470.3346790586422778No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG128500.32711842272406405No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA126430.3218488885992484No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC125910.3205251408963961No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC120440.30660033332985426No Hit
TGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC120140.3058366327320549No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA109490.2787252615101772No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC106060.26999361800867105No Hit
TCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT96710.24619161604392398No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT95190.2423221996817405No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGATTGCTGTAGGCACCATCA93220.23730723242285798No Hit
GGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA92750.23611076815297227No Hit
TGGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCT91380.2326232020896885No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATC91180.23211406835782225No Hit
ATGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC88030.22409521208092884No Hit
TAACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCA86010.21895296138907974No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATC83480.21251241968097173No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATAT83170.21172326239657902No Hit
CTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAATATCTCG78060.1987148955473964No Hit
TTTTTCTGATGTCTAACCTAGAACCATGCTGTAGGCACCATCAATATCTC74150.1887613310894113No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAAT73480.18705573308765933No Hit
TGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAATATC72550.1846882612344813No Hit
TGTTGTTTTCTGATGTCTAACCTAGAACCATGCTGTAGGCACCATCAATA72180.18374636383052873No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT72090.1835172536511889No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG68520.1744292165373764No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAATAT68300.17386916943232353No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG66170.168446895187948No Hit
ACTTACTGGAGGTCCGAACCGATTAACGCTGTAGGCACCATCAATATCTC66160.16842143850135466No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCACTGTAGGCACC64360.16383923491455843No Hit
ACATCCTGGGGCTGGAGCAGGTCCCAAGGGTTTGGCTGCTGTAGGCACCA64000.16292279419719918No Hit
TTAGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATAT63830.1624900305251129No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCA63500.16164995986753358No Hit
TGTTGTTTTCTGATGTCTAACCTAGAACCACTGTAGGCACCATCAATATC62950.1602498421049014No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCCTGTAGGCACCA61990.1578060001919434No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC61390.1562785989963447No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA61160.15569309520469848No Hit
GGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAATATCT58770.14960894710889683No Hit
TTGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATC57550.14650323134451274No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATCAATATC55770.1419719411309031No Hit
GGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTCG55130.14034271318893113No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATCTC54980.13996086289003146No Hit
ATGGCGAAAGCTAAGTTTGAGCGGAATCTGTAGGCACCATCAATATCTCG54540.1388407686799257No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAA54500.13873894193355246No Hit
TAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATC54440.1385862018139926No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC52880.13461495870543586No Hit
CGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGTA52490.13362214792829666No Hit
AACGGGGCTCAAGACATGCACCGAAGCTTAGGATTGCTGTAGGCACCATC51580.13130558944830523No Hit
ATGGCTTCTTCAACTTTTTATATTCCTTCTGTAGGCACCATCAATATCTC51530.13117830601533867No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA50950.12970181819292656No Hit
AAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCA49960.12718160622018862No Hit
TCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAATATCTCGT49410.12578148845755643No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA49190.12522144135250357No Hit
TGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATCT49000.12473776430723063No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT48830.12430500063514434No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCATCTGTAGGCACCA48100.12244666251383252No Hit
TGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCGT47760.12158113516965992No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT47650.12130111161713347No Hit
ACGATGATAACTAGCTGTCCGGGTACACTGTAGGCACCATCAATATCTCG47250.12028284415340099No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA46500.11837359265890256No Hit
AACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAA46430.11819539585274937No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTGTAGGCACCATCAAT43360.11038019306860244No Hit
TGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAATATCT43060.10961649247080307No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAAT40900.10411784816664763No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACCA40080.102030399865996No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACTGTAGGCACCATC39960.10172491962687626No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATAT39800.10131761264138325No Hit
ACTTACTGGAGGTCCGAACCGATTAACGTTGCTGTAGGCACCATCAATAT39400.10029934517765077No Hit

[FAIL]Adapter Content

Adapter graph