FastQCFastQC Report
Tue 18 Jul 2023
SRR10400976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10400976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5072376
Total Bases258.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA4080188.043922611415242No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT2303364.5409882863573205No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT1594873.1442266898195244No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC1233112.4310303494851326No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC762331.5029051474102078No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC732851.4447864275045856No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA609041.200699632677073No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC571731.127144359960697No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA516941.0191279195390879No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA512921.0112026395519575No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT420120.8282509025356164No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC393040.7748636930700721No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA390830.7705067605398338No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC340550.6713816168202041No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAAT282270.5564847716336486No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA280090.5521869829839112No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT258930.5104708326038921No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA258700.510017396186718No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA257030.5067250535054972No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT256620.5059167538053173No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCACTGTAGGCACCAT239820.4727961807247728No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCA239770.4726976075906044No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT234730.4627614356664411No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC233570.4604745389537369No Hit
GGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCTC230870.45515158970864933No Hit
TGTTGTTTTCTGATGTCTAACCTAGAACCACTGTAGGCACCATCAATATC229360.4521746810567671No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCCTGTAGGCACCA221270.43622554794833823No Hit
TGTTGTTTTCTGATGTCTAACCTAGAACCATGCTGTAGGCACCATCAATA182310.3594173618044088No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAATAT180110.3550801439010042No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA176210.34739143943587775No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCAA175000.3450059695890052No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA174340.34370480421798383No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC171700.3385001427338983No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGATTGCTGTAGGCACCATCA169310.3337883469206542No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT163940.32320159231098006No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCTGTAGGCACCAT162950.321249844254448No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATAT150780.2972571433978869No Hit
TTTTTCTGATGTCTAACCTAGAACCATGCTGTAGGCACCATCAATATCTC148220.2922101989284706No Hit
TTAACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATC148180.292131340421136No Hit
GGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA146640.2890952878887527No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCCTGTAGGCACCATCA143520.2829443243166516No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC132120.2604696497262821No Hit
TGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC132030.2602922180847792No Hit
TCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT130810.257887033611073No Hit
TTAGCTAACTGGTCCTAATTGCTCTATTCACGCCTGTAGGCACCATCAAT124480.24540767482536785No Hit
GTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCG124310.24507252616919567No Hit
TTCGCCAATTAAAGTGGTACGTGAGCTGGGTTCTGTAGGCACCATCAATA122680.2418590419953095No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT120430.23742325095773656No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC120380.23732467782356828No Hit
AACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAA120260.2370881023015644No Hit
TTGTTGTTTTCTGATGTCTAACCTAGAACCACTGTAGGCACCATCAATAT116550.22977397574627748No Hit
TGGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCT115560.22782222768974542No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATC111900.22060667426862676No Hit
ACGATGATAACTAGCTGTCCGGGTACACTGTAGGCACCATCAATATCTCG111780.2203700987466229No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC107660.21224767249115603No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG104410.20584041877021736No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCAA99780.19671254654623394No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC98440.19407078655052384No Hit
TTGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATC97040.1913107387938118No Hit
TTGTTGTTTTCTGATGTCTAACCTAGAACCATGCTGTAGGCACCATCAAT94290.18588921641455602No Hit
ATGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC92000.18137456686964845No Hit
TTAGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATAT89590.176623341802737No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG88850.17516445941704636No Hit
TTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA87030.17157639733332072No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA84170.1659380140588947No Hit
ACTTACTGGAGGTCCGAACCGATTAACGCTGTAGGCACCATCAATATCTC83860.1653268606270513No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATCAATATC82250.16215280570683246No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTGTAGGCACCATCAAT81780.16122621824565056No Hit
AGTACCGGGAGAATAAGCTCCGGCTAACTCCGCTGTAGGCACCATCAATA79180.15610041526889962No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT77510.15280807258767884No Hit
ACTTACTGGAGGTCCGAACCGATTAACGTTGCTGTAGGCACCATCAATAT75970.14977202005529558No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATC73070.14405477827353494No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT72050.14204388633650186No Hit
AAGACATGCACCGAAGCTTAGGATTGTCTGTAGGCACCATCAATATCTCG71160.14028928454830636No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACTGTAGGCACCATCAATA71000.13997385051896782No Hit
TAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAAT70800.13957955798229468No Hit
GTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAAT70710.13940212634079177No Hit
AACACATGTAGCTAACTGGTCCTAATTGCTCTCTGTAGGCACCATCAATA70400.1387909729089484No Hit
AAGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAATA69860.13772638305993087No Hit
TAACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCA69080.1361886421669056No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATAT68180.13441432575187645No Hit
TTAGCTAACTGGTCCTAATTGCTCTATTCACGCTCTGTAGGCACCATCAA68020.13409889172253792No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT67490.1330540165003541No Hit
AAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT62900.12400500278370531No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT60620.1195100678656314No Hit
GGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA60520.11931292159729483No Hit
GGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT59560.11742031742126373No Hit
CGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT59070.11645430070641452No Hit
TGTTGTTTTCTGATGTCTAACCTAGAACCATCTGTAGGCACCATCAATAT58080.11450255264988242No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCCTGTAGGCACCATCAA56110.1106187711636519No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG56040.11048076877581631No Hit
TAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATC55490.10939646429996513No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCAATATCTC54700.1078390087801062No Hit
CTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAATATCTCG52950.10438894908421616No Hit
CCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT52870.1042312320695469No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAA52800.10409322968171128No Hit
AAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCA52680.10385665415970739No Hit
TAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT52300.10310749834002843No Hit
GAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC52070.10265406192285431No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACCA51350.10123460879083096No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA51260.10105717714932805No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG51190.10091917476149244No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)