FastQCFastQC Report
Tue 18 Jul 2023
SRR10400978_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10400978_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3607431
Total Bases183.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA1105483.0644522376172962No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT447311.2399682765935094No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT444691.2327054904168644No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC442671.2271059377157871No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA338260.9376755924091132No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC311740.8641606727890291No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG310320.8602243535635193No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC308580.8554009764843735No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCACTGTAGGCACCAT305920.8480273080760242No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG285460.791311046559172No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA255380.7079276083173871No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCTGTAGGCACCAT243240.674274850995071No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC232450.6443643689927818No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT218490.605666470127911No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT215760.5980987578140788No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC208030.576670766537184No Hit
TTAGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATAT195760.5426576419618283No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT187440.519594137767292No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC177820.49292696104235956No Hit
TGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCGT155930.4322466597420713No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCAA150680.4176933668308555No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA143950.3990374313465732No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACCA141510.3922736152125987No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC136360.37799752788064417No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA120850.3350029425372239No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC114700.3179547994126568No Hit
ACTTACTGGAGGTCCGAACCGATTAACGTTGCTGTAGGCACCATCAATAT109660.30398363821788965No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA107560.2981623210534034No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT106730.295861514745535No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAATAT98810.2739068328680438No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC96480.2674479428712566No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCCTGTAGGCACCATCA93710.2597693483257199No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT92930.25760714480748215No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC92470.25633199914288035No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATAT89310.24757230283822476No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA85770.23775922533237642No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG84170.23332393606419638No Hit
GGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCTC79670.22084968499744No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA78030.21630351349755547Illumina PCR Primer Index 6 (96% over 29bp)
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT77990.21619263126585095No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA77110.21375322216835194No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT75430.2090961684367629No Hit
TAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATC75070.20809822835142236No Hit
AGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATATCT74850.20748837607704762No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA73960.2050212464216225No Hit
TTAGCTTTGGTGAAGCGAACCCGGCGAACTCTGTAGGCACCATCAATATC70150.19445971385176875No Hit
AAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCA70140.19443199329384264No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA69410.19240839256523548No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC69330.1921866281018265No Hit
TTGGGAAGGATATTACCAAAGTGAATACTGTAGGCACCATCAATATCTCG67160.1861712670318573No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT66670.18481295969347716No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATC64310.1782709080229116No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCCTGTAGGCACCA62940.17447319158703242No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATAT60640.1680974632640236No Hit
GGGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCAT59190.16407798236473545No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCCTGTAGGCACCATCAA57860.1603911481605608No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT57040.15811806241061854No Hit
ATGTCGGCTCATCACATCCTGGGGCTGGCTGTAGGCACCATCAATATCTC56320.1561221822399375No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCGCTGTAGGCACCATCAAT54950.15232446580405834No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCTGTAGGCACCAT53570.14849902881025306No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAA52270.14489535627985678No Hit
CTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC51700.14331528447806766No Hit
GGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA51280.14215102104517038No Hit
TGGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCT50640.14037690533789834No Hit
TAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT50380.13965617083181908No Hit
ACGATGATAACTAGCTGTCCGGGTACACTGTAGGCACCATCAATATCTCG49800.13804837847210386No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATCAATATC49640.13760484954528585No Hit
TTGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATC48670.13491595542645168No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCA47130.13064698950582837No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA47100.13056382783205No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATAT46930.1300925783473059No Hit
ATCTAAGTACCCGGAGGAAAAGACACTGTAGGCACCATCAATATCTCGTA46920.13006485778937976No Hit
AAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC46840.12984309332597074No Hit
TTGCGTAATGGAAACCTTCACGGGTAGACATTACCTGTAGGCACCATCAA46580.12912235881989148No Hit
AGGTGGGAAGGATATTACCAAAGTGCTGTAGGCACCATCAATATCTCGTA46560.12906691770403925No Hit
TGACCAGAAGGTCGGGCGGCCGCCGGTATGCTGTAGGCACCATCAATATC44890.12443758453037632No Hit
TTAGCTTTGGTGAAGCGAACCCGGCGAACCTGTAGGCACCATCAATATCT44370.12299611551821782No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT44340.12291295384443944No Hit
AGGATGAGTCCTTCTTTGTAAAAAGGAGGACTGTCCTCTGTAGGCACCAT43890.1216655287377638No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTGTAGGCACCATCAAT43780.12136060260057643No Hit
AAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT42600.11808957676529364No Hit
ACACCGGTGATATGACTGGCGTTCTCGGCTGTAGGCACCATCAATATCTC42500.11781237118603238No Hit
TGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATCT42220.11703619556410087No Hit
TCCAGAAGCATATCACTTCTGACCACACGCTGTAGGCACCATCAATATCT41820.11592737324705588No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCA41780.11581649101535137No Hit
TCAGCTCGTGTCGTGAGATGTTGGGTTCTGTAGGCACCATCAATATCTCG41760.11576104989949913No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCACTGTAGGCACC41760.11576104989949913No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC41450.11490171260378923No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACTGTAGGCACCATC40840.1132107585702956No Hit
ATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCGTATG39380.1091635571130813No Hit
AACACATGTAGCTAACTGGTCCTAATTGCTCTCTGTAGGCACCATCAATA38830.10763892642714443No Hit
TTAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCAT38620.10705679471069579No Hit
GTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCG38180.10583709016194628No Hit
TAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAAT37900.10506091454001477No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACTGTAGGCACCATCAATA37570.10414613612845262No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAATA36310.10065334582976083No Hit
AAGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAATA36140.10018209634501671No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)