FastQCFastQC Report
Tue 18 Jul 2023
SRR10400982_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10400982_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4338395
Total Bases221.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT1515303.492766334093599No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCACTGTAGGCACCAT1049152.418290635131195No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA1045552.4099926355253496No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCTGTAGGCACCAT959122.210771494988354No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC696871.6062852737014492No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC475071.095036297985776No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA403510.9300905058207011No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCTGTAGGCACCAT390490.9000794072462283No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACCA381480.8793113582327104No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT352190.8117979114395992No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA313910.7235625156307806No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA286200.6596909686646789No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT279890.6451464193555451No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCAA268210.618224020634359No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC248230.5721701228219191No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA248150.5719857228306782No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC246920.5691505729653478No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA223540.5152596755251654No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT205690.4741154274795172No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA195790.4512959285634434No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT186990.43101192952693335No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC180360.41572978025283547No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCCTGTAGGCACCA171490.3952844312239895No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC161460.37216528232214907No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT151700.349668483390747No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT149950.34563473358235014No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT146870.33853533391957163No Hit
TTAGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATAT143870.3316203342480341No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC138050.3182052348852514No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA133130.3068646354239298No Hit
TAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATC130590.30100993570202805No Hit
AGGATGAGTCCTTCTTTGTAAAAAGGAGGACTGTCCTCTGTAGGCACCAT121370.2797578367115027No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA116670.268924337226094No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCA115280.2657203873782816No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA114010.2627930375173307No Hit
AAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCA110130.2538496379421422No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC105370.2428778384633027No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCATCTGTAGGCACCA103990.23969693861439542No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA102760.23686178874906505No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC102440.23612418878410105No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATAT96820.22317008939942076No Hit
ACTTACTGGAGGTCCGAACCGATTAACGTTGCTGTAGGCACCATCAATAT95460.22003528954832374No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC94930.2188136396063521No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACTGTAGGCACCATC94170.2170618396895626No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT92560.2133507898658375No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATAT91550.21102273997641985No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTGTAGGCACCATCAAT86040.19832219057969594No Hit
TCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT85710.19756154061582681No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA84360.19444979076363494No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG79980.18435389124319015No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC78590.18114994139537777No Hit
TGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC77230.17801514154428077No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACTGTAGGCACCATCAATA75820.17476509169865814No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC75300.17356649175559166No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG74470.17165334184646625No Hit
TCCAGAAGCATATCACTTCTGACCACACGCTGTAGGCACCATCAATATCT73210.1687490419844205No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG71880.16568339213003888No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA71430.16464614217930826No Hit
ATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCTC69630.16049714237638574No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT68650.15823824248368348No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAATAT68270.15736234252528872No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC67170.15482684264572497No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAA65370.15067784284280247No Hit
GGGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCAT64820.14941009290302057No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT64100.14775049298185158No Hit
TATCCTGGGGCTGGAGCAGGTCCCAAGGGTTTGGCTGCTGTAGGCACCAT64050.14763524298732597No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATC63650.14671324303112096No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACTGTAGGCACCATC63640.14669019303221584No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT61310.1413195432873217No Hit
TTGGGAAGGATATTACCAAAGTGAATACTGTAGGCACCATCAATATCTCG61040.14069719331688332No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATC60840.14023619333878082No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACC59570.13730884347782993No Hit
TAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT59540.13723969348111456No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT59520.13719359348330432No Hit
AAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT57700.13299849368257155No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA57500.13253749370446904Illumina PCR Primer Index 6 (96% over 29bp)
AGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATATCT56840.1310161937767308No Hit
TAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAAT56730.13076264378877442No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATAT56680.1306473937942488No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATC55160.12714379396066977No Hit
AAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT54900.12654449398913653No Hit
ATGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC54900.12654449398913653No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAA54660.12599129401541354No Hit
GGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA53870.12417034410190865No Hit
TTGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATC53820.12405509410738304No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG52990.12214194419825765No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCA52130.1201596442924169No Hit
AAGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAATA51800.11939899432854777No Hit
TTAGCTTTGGTGAAGCGAACCCGGCGAACTCTGTAGGCACCATCAATATC51290.1182234443843864No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT50940.11741669442270701No Hit
TTTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTC50550.11651774446540715No Hit
ATGTCGGCTCATCACATCCTGGGGCTGGCTGTAGGCACCATCAATATCTC49930.11508864453328939No Hit
TTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATATCTCG48330.11140064470846937No Hit
AAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC48160.11100879472708226No Hit
ACACCGGTGATATGACTGGCGTTCTCGGCTGTAGGCACCATCAATATCTC47600.10971799478839525No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCGCTGTAGGCACCATCAAT47230.10886514482890562No Hit
TTAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCA46590.10738994489897763No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCTGTAGGCACCA46410.10697504491868537No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT45190.1041629450522601No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCCTGTAGGCACCATCAA44240.10197319515627323No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATC43980.10137389518473999No Hit
AAGGATGAGTCCTTCTTTGTAAAAAGGAGGACTGTCCTCTGTAGGCACCA43640.10059019522196573No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)