FastQCFastQC Report
Tue 18 Jul 2023
SRR10400990_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10400990_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3944066
Total Bases201.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA1612254.087786563409436No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT1591574.035353363762169No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT715741.814726224155478No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA597211.514198798904481No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA497401.2611350824250913No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC488731.2391526916638818No Hit
TTAACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATC457991.161212819460932No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC402161.01965839314048No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA399241.0122548659175583No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC358710.9094928938815934No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC330450.83784094890907No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT328970.8340884762070412No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA284280.7207790133329413No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT260690.6609676410080358No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC260000.6592181773834414No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT238940.6058215050153826No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT233160.591166577841243No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA220330.5586366962418986No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA214790.5445902781545745No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT207370.5257772055538624No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCACTGTAGGCACCAT204050.5173594965195816No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC201760.5115533056495505No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT189730.48105178767292434No Hit
GGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCTC159880.4053684700002485No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC145650.36928895206114704No Hit
GTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCG144940.3674887793459846No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCAA137440.3484728703830007No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC135690.3440358249583044No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG133280.3379253795448656No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA132930.33703797045992634No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT130980.33209383412955057No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA129410.32811317051996597No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC125870.31913766148943756No Hit
TGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC120130.3045841524964339No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC119490.30296146159825926No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA113530.2878501526090081No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC110810.2809537162917659No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT97890.24819564378486567No Hit
TCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT97770.24789138924145795No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGATTGCTGTAGGCACCATCA94650.23998077111285665No Hit
TGGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCT93820.2378763438542864No Hit
GGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA93440.2369128711334952No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATC91680.232450471163515No Hit
ATGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC89100.22590899848024854No Hit
TAACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCA86380.2190125621630064No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATAT85070.2156911167308052No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATC81610.20691844406254867No Hit
CTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAATATCTCG77280.19593992595458593No Hit
TTTTTCTGATGTCTAACCTAGAACCATGCTGTAGGCACCATCAATATCTC75930.19251706234124885No Hit
TGTTGTTTTCTGATGTCTAACCTAGAACCATGCTGTAGGCACCATCAATA73920.18742079873916917No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT73870.18729402601274928No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAAT73480.18630519874667412No Hit
TGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAATATC71660.18169067150499002No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAATAT69200.17545345336513132No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG68420.17347579883298098No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG66800.16936836249697645No Hit
ACTTACTGGAGGTCCGAACCGATTAACGCTGTAGGCACCATCAATATCTC65890.1670610988761344No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCACTGTAGGCACC65180.16526092616097196No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCA64270.1629536625401299No Hit
TTAGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATAT63320.16054498073815193No Hit
TGTTGTTTTCTGATGTCTAACCTAGAACCACTGTAGGCACCATCAATATC63050.1598604080154845No Hit
ACATCCTGGGGCTGGAGCAGGTCCCAAGGGTTTGGCTGCTGTAGGCACCA62240.15780668984748228No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC61740.15653896258328334No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA61100.15491627168510871No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCCTGTAGGCACCA60600.15364854442090978No Hit
TTGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATC60530.15347106260392196No Hit
GGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAATATCT59460.15075812625853624No Hit
ATGGCGAAAGCTAAGTTTGAGCGGAATCTGTAGGCACCATCAATATCTCG57330.14535760811304882No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATCTC57040.14462232629981345No Hit
GGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTCG55630.1410473354147725No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAA55080.13965283542415363No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATCAATATC54970.13937393542602988No Hit
TAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATC54790.1389175536109183No Hit
CGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGTA54240.13752305362029946No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC52250.13247749910878773No Hit
ATGGCTTCTTCAACTTTTTATATTCCTTCTGTAGGCACCATCAATATCTC51870.13151402638799653No Hit
AACGGGGCTCAAGACATGCACCGAAGCTTAGGATTGCTGTAGGCACCATC51370.13024629912379762No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA51360.13022094457851363No Hit
TGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATCT51300.13006881730680978No Hit
AAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCA51130.12963779003698214No Hit
TCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAATATCTCGT50850.12892786276903073No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA50620.12834470822749924No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCATCTGTAGGCACCA50140.12712769005386826No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT48950.12411049916507483No Hit
TGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCGT48560.12312167189899967No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT48020.12175252645366481No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA45930.11645342648931331No Hit
ACGATGATAACTAGCTGTCCGGGTACACTGTAGGCACCATCAATATCTCG45920.11642807194402933No Hit
AACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAA45470.1152871174062503No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTGTAGGCACCATCAAT44760.11348694469108783No Hit
ACTTACTGGAGGTCCGAACCGATTAACGTTGCTGTAGGCACCATCAATAT43460.11019085380417062No Hit
TGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAATATCT42800.10851745381542804No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAAT42040.10659050837384568No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACCA41420.105018526566239No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACTGTAGGCACCATC40550.10281268112653287No Hit
CCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT39520.10020116296228308No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)