FastQCFastQC Report
Tue 18 Jul 2023
SRR10400993_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10400993_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5117925
Total Bases261 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA4252148.308328082181744No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT2348304.588382987245808No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT1609663.145141829940845No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC1245492.4335839231719887No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC787721.5391393973143412No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC749201.4638745194585696No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA621211.213792699189613No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC584661.1423770375689366No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA524661.025142025332532No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA522751.0214100441096734No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT435260.8504618571002897No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA399790.7811564256998687No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC394420.7706638921047104No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC354880.6934060190409199No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAAT288470.5636463996639263No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA287640.5620246486613227No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA262850.5135870494389816No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT261890.5117112892431991No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA260600.5091907364801165No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT256880.5019221657214593No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCACTGTAGGCACCAT246200.4810543335433794No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCA242480.4737857627847223No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT237770.46458281432416454No Hit
GGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCTC236550.46219903574202437No Hit
TGTTGTTTTCTGATGTCTAACCTAGAACCACTGTAGGCACCATCAATATC233880.4569820776975044No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC232960.45518447417654617No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCCTGTAGGCACCA221700.4331833702135143No Hit
TGTTGTTTTCTGATGTCTAACCTAGAACCATGCTGTAGGCACCATCAATA186140.3637020862947386No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAATAT184280.3600678009154101No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCAA181190.3540301977852352No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA179720.3511579399854433No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC175990.34386983005808014No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA174760.3414665123072339No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGATTGCTGTAGGCACCATCA172460.33697250350483837No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCTGTAGGCACCAT167820.3279063292252231No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT165990.3243306613520128No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATAT152270.2975229218872883No Hit
TTAACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATC151400.2958230142098604No Hit
TTTTTCTGATGTCTAACCTAGAACCATGCTGTAGGCACCATCAATATCTC150480.29402541068890226No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCCTGTAGGCACCATCA149920.29293121724136245No Hit
GGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA147850.2888866093192065No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC135670.2650879018352164No Hit
TCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT134340.26248919239730945No Hit
TGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC133310.2604766580205845No Hit
GTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCG127760.24963241938871708No Hit
TTAGCTAACTGGTCCTAATTGCTCTATTCACGCCTGTAGGCACCATCAAT126650.24746357166234362No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT122660.23966744334862275No Hit
TTCGCCAATTAAAGTGGTACGTGAGCTGGGTTCTGTAGGCACCATCAATA121900.23818246652696162No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC120710.23585730545093958No Hit
AACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAA119380.23325859601303262No Hit
TTGTTGTTTTCTGATGTCTAACCTAGAACCACTGTAGGCACCATCAATAT116680.2279830204623944No Hit
TGGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCT114940.2245832051075387No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATC113880.22251205322469553No Hit
ACGATGATAACTAGCTGTCCGGGTACACTGTAGGCACCATCAATATCTCG113800.22235573987504703No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC110750.21639629341969646No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG104010.20322689371180705No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCAA102370.20002247004401197No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC100120.1956261570851468No Hit
TTGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATC96310.18818173380813513No Hit
TTAGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATAT93750.18317970661938188No Hit
TTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA92090.1799362046141747No Hit
ATGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC91190.17817767943062862No Hit
TTGTTGTTTTCTGATGTCTAACCTAGAACCATGCTGTAGGCACCATCAAT91170.1781386010932165No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG89640.17514910828118818No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATCAATATC85260.16659095238793067No Hit
ACTTACTGGAGGTCCGAACCGATTAACGCTGTAGGCACCATCAATATCTC83800.16373823375684482No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA83350.16285897116507178No Hit
AGTACCGGGAGAATAAGCTCCGGCTAACTCCGCTGTAGGCACCATCAATA81420.15908791160480076No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT80940.15815003150690954No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTGTAGGCACCATCAAT80330.15695814221583942No Hit
ACTTACTGGAGGTCCGAACCGATTAACGTTGCTGTAGGCACCATCAATAT75450.14742302788727854No Hit
GTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAAT74650.14585989439079314No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT74430.14543003267925966No Hit
AACACATGTAGCTAACTGGTCCTAATTGCTCTCTGTAGGCACCATCAATA73210.14304625409711944No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACTGTAGGCACCATCAATA72970.1425773140481738No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATC72770.14218653067405246No Hit
TAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAAT71600.1399004479354426No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATAT70660.13806376607707224No Hit
TAACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCA70020.13681325927988394No Hit
AAGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAATA69840.13646155424317474No Hit
AAGACATGCACCGAAGCTTAGGATTGTCTGTAGGCACCATCAATATCTCG69320.13544551747045921No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT67850.1325732596706673No Hit
TTAGCTAACTGGTCCTAATTGCTCTATTCACGCTCTGTAGGCACCATCAA67510.13190892793466102No Hit
AAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT62930.12295998866728217No Hit
GGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA61910.12096699345926328No Hit
GGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT61170.11952109497501429No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT60660.11852459737100485No Hit
CGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT59910.11705915971804978No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCCTGTAGGCACCATCAA58640.11457768529237924No Hit
TGTTGTTTTCTGATGTCTAACCTAGAACCATCTGTAGGCACCATCAATAT56400.11020091150222014No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG55660.10875501301797115No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCAATATCTC55560.10855962133091049No Hit
TAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATC54630.1067424786412462No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACCA54270.10603906856782779No Hit
CCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT53690.10490579678287587No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG53540.10461270925228486No Hit
CTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAATATCTCG53400.1043391608903999No Hit
TAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT53270.10408515169722105No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAA52960.10347943746733296No Hit
GAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC52890.10334266328639048No Hit
AAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCA52640.1028541840687388No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA52510.1026001748755599No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA52120.1018381472960233No Hit
ACACATGTAGCTAACTGGTCCTAATTGCTCTCTGTAGGCACCATCAATAT51370.10037270964306823No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)