FastQCFastQC Report
Tue 18 Jul 2023
SRR10400999_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10400999_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3827598
Total Bases195.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC1700904.443779101149076No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA1085542.8360867572822435No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT952692.489002241092194No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC861882.2517516207292405No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA782232.043657667288989No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC578461.5112872354933824No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT444221.1605711989608103No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC428871.1204677189192804No Hit
ATGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC360850.9427583565463249No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCACTGTAGGCACCAT353880.924548502742451No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA353430.9233728306891162No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC339500.8869792491269982No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG321540.8400568711761267No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC301180.7868642422741365No Hit
GGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCTC300400.7848264107150229No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT294640.769777808432338No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA288740.7543634415108379No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA237720.6210683567083064No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA219670.5739108443467679No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC207710.5426640937736931No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT207160.5412271612640617No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT200670.5242713576504116No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCTGTAGGCACCAT190510.4977272952906757No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATAT188600.4927372205754105No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACCA179140.468021981409751No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT171670.4485058253243941No Hit
TGGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCT169730.44343737247223974No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG165730.43298695422037525No Hit
ATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCTC160700.41984555326865564No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG148790.3887294329237292No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA145850.38104837550860876No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA139760.36513761372014514No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT121610.31771884090231No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC119580.3124152536394888No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA114660.29956123918969546No Hit
TTAGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATAT114400.2988819620033243No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA111170.2904432492649437No Hit
TTTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATAT110480.2886405521164971No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCA110350.2883009135233115No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCCTGTAGGCACCATCAA109190.2852702922302708No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC103190.2695946648524741No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCAA102340.26737395097395283No Hit
TTTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTC99680.26042442283646294No Hit
CTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC97600.25499020534549344No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT95420.24929472739822728No Hit
ACGATGATAACTAGCTGTCCGGGTACACTGTAGGCACCATCAATATCTCG93890.2452974424168891No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA92620.24197943462192215No Hit
TATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT91290.2385046705531772No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA90620.2367542254959899No Hit
TAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATAT90260.23581368785332213No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTCTGTAGGCACCATCA90160.23555242739702548No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAA87720.22917767226338817No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATC87250.22794974811879407No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT86950.22716596674990427No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG84840.22165337112204572No Hit
TTTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCT84630.22110472416382285No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA84390.22047769906871098No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCTGTAGGCACCAT83520.21820473309893043No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC80690.2108110621857363No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT79460.20759755857328802No Hit
ACTTACTGGAGGTCCGAACCGATTAACGTTGCTGTAGGCACCATCAATAT79010.20642188651995322No Hit
TGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCT77490.20245072758424473No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT76200.19908046769801843No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT75300.19672912359134892No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT74540.19474354412349468No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT71460.18669672206955903No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC71400.18653996579578105No Hit
GGGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCAT70260.18356159659399968No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAA69590.18181115153681238No Hit
CTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC67190.1755409005856937No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC66720.1743129764410996No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA66250.17308505229650553No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT64750.16916614545205635No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAAT61800.1614589619913063No Hit
AAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT61180.1598391471622673No Hit
AGGGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATC60920.15915986997589612No Hit
GATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT60230.1573571728274495No Hit
TGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCGT59500.1554499714964842No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCCTGTAGGCACCA59410.15521483708581726No Hit
TAGTCGAACAGAGACGAAAGTCGGTCATAGTGCTGTAGGCACCATCAATA59350.1550580808120393No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC58950.15401303898685284No Hit
ACCCACCCGATCCCATTTCGAACTCGGACGTGCTGTAGGCACCATCAATA58600.1530986273898147No Hit
GGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTC58490.15281124088788844No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCACTGTAGGCACC57370.14988512377736637No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATAT57000.14891846008906892No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAATA56040.14641035970862146No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATC53750.14042749525942902No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATATCT53440.13961758784490952No Hit
GTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT53370.13943470552550188No Hit
TCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT51050.1333734629394205No Hit
TTAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCA50430.1317536481103815No Hit
TTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATATCTCG50160.13104824487838065No Hit
GGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCAT49400.12906266541052638No Hit
AACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCATC48850.12762573290089504No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACTGTAGGCACCATC48070.12558790134178144No Hit
TTAACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATC47640.12446448137970602No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCTACTGTAGGCACCATCAA46070.1203626922158492No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCATCTGTAGGCACCA45220.11814197833732801No Hit
GGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTCG44460.11615639886947376No Hit
TCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTCG44100.11521586122680595No Hit
GAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA43930.1147717184511017No Hit
TGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATC43570.1138311808084339No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATCTC43070.11252487852695084No Hit
AGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATATCT42880.11202848365998727No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAAT42640.1114014585648754No Hit
ACCCGATCCCATTTCGAACTCGGACGCTGTAGGCACCATCAATATCTCGT42380.11072218137850422No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATC41860.10936362700576184No Hit
TGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC41420.10821408099805675No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCTACTGTAGGCACCATCAA40580.10601949316516521No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC40370.10547084620694232No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGGCTGTAGGCACCATCAATAT38790.10134293099745585No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA38280.10001050267034312No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)