FastQCFastQC Report
Tue 18 Jul 2023
SRR10401003_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10401003_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5670674
Total Bases289.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC3206515.6545482953172765No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC1618282.853770116215462No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT1386202.4445065965703545No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA930441.6407926112486804No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT798351.4078573375933794No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT679451.1981820855862988No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA628051.1075403029692767No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG622181.0971887997793561No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT620761.0946846882751504No Hit
ATGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC570451.005965075756427No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG462340.8153175442636977No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC388670.6854035340419852No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC374830.6609972641700088No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCCTGTAGGCACCATCAA353780.6238764563083683No Hit
TAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATAT335420.5914993526342724No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA328420.5791551409938218No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC306660.5407822773800786No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATAT266200.4694327340982747No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG254740.44922349618405144No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC249210.4394715689880956No Hit
ATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCTC241220.42538153312992427No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG236410.4168992962741289No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA228560.40305614464876666No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA227640.4014337625474503No Hit
GTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT220080.3881020139757637No Hit
TTTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATAT208720.3680691219421183No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCAA193790.3417406819718432No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT179970.31736968127598236No Hit
TGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCGT176970.31207930485864643No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT172270.3037910484714868No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT171980.303279645417811No Hit
GGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCTC165680.2921698549414055No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA165230.2913762984788052No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA164610.2902829540192224No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA163440.2882197072164614No Hit
TGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATCT158140.2788733755458346No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTCTGTAGGCACCATCA151790.2676754121291402No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT151220.2666702406098464No Hit
TGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCT147260.25968694373896295No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT146920.2590873677449982No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA146230.25787058116901096No Hit
AGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATATC141300.24917672925652223No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT137230.24199945191700317No Hit
AAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT137050.241682029331963No Hit
AGGATGAGTCCTTCTTTGTAAAAAGGAGGACTGTCCTCTGTAGGCACCAT130580.2302724508585752No Hit
AACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCATC127780.22533476620239498No Hit
CTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC127630.2250702473815282No Hit
ACCTGCGGCTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTC125850.2219312907072422No Hit
TATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT125680.22163150271025983No Hit
TTAGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATAT124760.2200091206089435No Hit
TTTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTC124320.21923319873440086No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAA120750.21293765079777113No Hit
TGGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCT116770.20591908475077214No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAATAT115500.20367949206743324No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT114410.20175732196913454No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT113980.20099903468264974No Hit
TTAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCA109360.1928518549999524No Hit
TTTGGACAATGGGGGAAACCCTGATCCAGCCTGTAGGCACCATCAATATC107200.18904278397947052No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA104620.18449306026056161No Hit
GATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT104480.18424617602775262No Hit
TAGTCGAACATATCGTTAGATAGTTTGACCTGTAGGCACCATCAATATCT100410.17706889868823353No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA100410.17706889868823353No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC98500.17370069236919633No Hit
TTGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATC97950.17273079002601807No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA95510.1684279505399182Illumina PCR Primer Index 6 (96% over 29bp)
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC93280.16449543740303182No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC92960.16393113058518263No Hit
ACCCGATCCCATTTCGAACTCGGACGCTGTAGGCACCATCAATATCTCGT89710.15819988946640207No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCA89540.15790010146941968No Hit
TTTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCT87790.15481404855930705No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAATA86110.15185143776559895No Hit
TCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT85100.1500703443717625No Hit
TGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATC84400.14883592320771746No Hit
TTAAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCT84240.1485537697987929No Hit
TGGATGAAGGTAAGGTAACACTTACTGGAGGTCCGCTGTAGGCACCATCA83600.14742515616309454No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTTCTGTAGGCACCATC83060.1464728884079741No Hit
GGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTC82390.14529137100810238No Hit
CTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC81420.1435808159664971No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGACTGTAGGCACCATCAATATCTCG79000.1393132456565128No Hit
TGACGGAAACACTCCTTTTCAAAGGGGTGTTTCTTCTGTAGGCACCATCA78600.13860786213420132No Hit
AGTAGGAGGGTCTTTAAGGCCTGACTGCGCTGTAGGCACCATCAATATCT78530.13848442001779682No Hit
TTTGGACAATGGGGGAAACCCTGATCCAGCTGTAGGCACCATCAATATCT75980.1339876000630613No Hit
GGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTCG74150.13076047044848638No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT73720.13000218316200157No Hit
TACCCGATCCCATTTCGAACTCGGACGCTGTAGGCACCATCAATATCTCG73340.12933206881580567No Hit
TGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC71760.12654580390267542No Hit
ACTGCTTGGTGGGGCTTGCTCTGCTAGGCGGCTGTAGGCACCATCAATAT71160.12548772861920823No Hit
TTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATATCTCG70080.1235831931089673No Hit
TTGACGGAAACACTCCTTTTCAAAGGGGTGTTTCTTCTGTAGGCACCATC70020.12347738558062059No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAAT67790.1195448724437342No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCTACTGTAGGCACCATCAA67440.11892766186171166No Hit
GTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC67320.11871604680501824No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCTACTGTAGGCACCATCAA67040.11822227833940022No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGGCTGTAGGCACCATCAATAT65970.11633537741721708No Hit
ATGGGGAAACCCATCCTCACCATTTAATTCTGTAGGCACCATCAATATCT65810.11605322400829249No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT65060.1147306299039585No Hit
CAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT63750.1124204988683885No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC62330.10991638736418281No Hit
TTTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATC60720.1070772186868792No Hit
AAGGATGAGTCCTTCTTTGTAAAAAGGAGGACTGTCCTCTGTAGGCACCA60320.10637183516456773No Hit
GGAGGTTGGCTTAGAAGCAGCCATCCTTCTGTAGGCACCATCAATATCTC59960.10573698999448743No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACCA59540.1049963372960604No Hit
CGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGTA57240.10094038204276953No Hit
AGGTGGGAAGGATATTACCAAAGTGCTGTAGGCACCATCAATATCTCGTA57160.10079930533830722No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)