FastQCFastQC Report
Tue 18 Jul 2023
SRR10401005_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10401005_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3835007
Total Bases195.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA2689907.014068031688078No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT1270933.3140226341177477No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCTGTAGGCACCAT1140662.9743361615767587No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT908812.369774031703202No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA865272.2562409925197007No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC743721.9392924185014526No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC633301.6513659557857392No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC548631.4305840901985316No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT495341.29162736860715No Hit
ACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATATCT348260.9081078600378043No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACCA341330.8900374888494336No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA323380.8432318376472325No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACTGTAGGCACCATC321280.8377559675901505No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA314730.8206764681263946No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC291920.7611980890778035No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC260910.6803377412348922No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC252260.6577823717140542No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA249610.6508723452134507No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATAT222290.5796338833279835No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT211780.5522284574708729No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA206070.5373393060299498No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA202680.5284996872235175No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCA189440.49397563029220026No Hit
TTAGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATAT173560.4525676224319799No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA169220.44125082431401036No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT168320.4389040228609752No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCAA160360.41814786778746427No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATC155600.40573589565807827No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCTGTAGGCACCA153030.3990344737310779No Hit
TGTTGTTTTCTGATGTCTAACCTAGAACCATGCTGTAGGCACCATCAATA153010.39898232258767713No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC151010.39376720824759903No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG142420.3713682921569635No Hit
CACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATATC134900.3517594622382697No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT134420.350507834796651No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCTGTAGGCACCATC130010.33900850767677865No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAA123950.3232067112263419No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT123150.3211206654903107No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA119890.31262002911598336No Hit
TTAGCTTTGGTGAAGCGAACCCGGCGAACCTGTAGGCACCATCAATATCT116250.3031285210170412No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCTGTAGGCACCA108130.281955156796324No Hit
TGTTGTTTTCTGATGTCTAACCTAGAACCACTGTAGGCACCATCAATATC107940.2814597209340165No Hit
CGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATATCTCGTAT100850.2629721405984396No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCA98320.2563750209582408No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATAT98160.2559578118110345No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATCAATATC94300.2458926411346837No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC91040.23739200476035638No Hit
TTGTTGTTTTCTGATGTCTAACCTAGAACCATGCTGTAGGCACCATCAAT85850.22385878304785364No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT85750.2235980273308497No Hit
TCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT85560.2231025914685423No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAA85450.222815760179838No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCTGTAGGCACCAT84860.22127730144951493No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAATAT84530.22041680758340207No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC82820.21595788482263525No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC81560.21267236278838605No Hit
TGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC80460.20980404990134308No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAAT80170.20904785832203174No Hit
TTAGCTTTGGTGAAGCGAACCCGGCGAACTCTGTAGGCACCATCAATATC77770.202789721113938No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTGTAGGCACCATCAAT75620.19718347319835403No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACTGTAGGCACCATCAATA74560.19441946259811257No Hit
TGCACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATA74280.19368934659050166No Hit
AACACATGTAGCTAACTGGTCCTAATTGCTCTCTGTAGGCACCATCAATA70720.1844064430651626No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACTGTAGGCACCAT70140.18289405990653992No Hit
TCACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATAT69560.18138167674791728No Hit
AGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATATCT68100.17757464327966024No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT67070.17488885939452No Hit
TTTTTCTGATGTCTAACCTAGAACCATGCTGTAGGCACCATCAATATCTC66940.17454987696241495No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACTGTAGGCACCATCA66360.17303749380379227No Hit
ACTTACTGGAGGTCCGAACCGATTAACGCTGTAGGCACCATCAATATCTC65960.17199447093577666No Hit
ACTTACTGGAGGTCCGAACCGATTAACGTTGCTGTAGGCACCATCAATAT65390.17050816334885438No Hit
TAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAAT65010.16951729162423954No Hit
GGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA62770.16367636356335202No Hit
TAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATC62250.16232043383493172No Hit
AAGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAATA61910.16143386439711843No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGATTGCTGTAGGCACCATCA61270.15976502780829344No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATATCT60650.15814834236286923No Hit
ACACATGTAGCTAACTGGTCCTAATTGCTCTCTGTAGGCACCATCAATAT60420.15754860421376024No Hit
GTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCG60320.15728784849675634No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACC59780.15587976762493522No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAAT59710.1556972386230325No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACCAT59630.15548863404942936No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC58950.1537154951738028No Hit
TTGTTGTTTTCTGATGTCTAACCTAGAACCACTGTAGGCACCATCAATAT58560.15269854787748757No Hit
ACCTGCGGCTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTC57930.15105578686036297No Hit
AAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCA56120.14633610838259226No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC55900.14576244580518366No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG55760.1453973878013782No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC53680.13997366888769694No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA52970.1381223032969692Illumina PCR Primer Index 6 (96% over 29bp)
AAGACATGCACCGAAGCTTAGGATTGTTACCTGTAGGCACCATCAATATC50450.13155125922847077No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG50250.13102974779446297No Hit
TTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA50060.13053431193215553No Hit
TAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT49980.13032570735855242No Hit
TTGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATC48460.12636222046009302No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA47740.12448477929766491No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT47150.12294632056734185No Hit
CTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC46300.12072989697280866No Hit
GGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAATATCT46120.12026053668220162No Hit
CGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGTA45790.11940004281608874No Hit
TTGCACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAAT43270.1128289987475903No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCGCTGTAGGCACCATCAAT43210.11267254531738796No Hit
GGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCTC42560.11097763315686254No Hit
AACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAA40510.10563214095828247No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATCTC40480.1055539142431813No Hit
GTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAAT40480.1055539142431813No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCAA39500.10299850821654302No Hit
TGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCG39500.10299850821654302No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT38670.10083423576541059No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)