FastQCFastQC Report
Tue 18 Jul 2023
SRR10401006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10401006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3833982
Total Bases195.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA2683957.000424102147584No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT1264423.297928889598334No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCTGTAGGCACCAT1137022.9656372930285015No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT907242.3663126222293167No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA865582.2576527485001234No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC746941.9482094595123296No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC628601.6395486468115918No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC542761.4156560985419337No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT499551.3029534306629504No Hit
ACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATATCT350270.9135932302238248No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACCA340600.8883714112377157No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA319250.8326851821422219No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACTGTAGGCACCATC316750.8261645464167541No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA313950.8188614344042303No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC289940.7562372488968389No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC261610.6823454048558392No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA249810.6515680042316317No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC249740.6513854264313186No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATAT222030.579110700050235No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT211200.550863306087509No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA205450.5358658439189334No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA204040.5321882053697695No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCA193130.5037321510638287No Hit
TTAGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATAT170570.4448899342772084No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT169300.4415774513286708No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA169240.4414209560712596No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCAA159330.41557315605550577No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATC157140.4098610791599961No Hit
TGTTGTTTTCTGATGTCTAACCTAGAACCATGCTGTAGGCACCATCAATA153490.40034095100081324No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC150040.39134247369966785No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCTGTAGGCACCA149400.38967319095394815No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG142380.3713632458368349No Hit
CACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATATC136830.3568874345262967No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT135110.3524012371471749No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCTGTAGGCACCATC126300.32942251685062685No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAA125180.32650127204561735No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT122660.3199284712343459No Hit
TTAGCTTTGGTGAAGCGAACCCGGCGAACCTGTAGGCACCATCAATATCT118080.30798266658528917No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA117640.3068350346976068No Hit
TGTTGTTTTCTGATGTCTAACCTAGAACCACTGTAGGCACCATCAATATC111380.2905073628410358No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCTGTAGGCACCA108440.2828390952278858No Hit
CGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATATCTCGTAT100520.2621817212496042No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATAT98480.25686088249762257No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCA98130.2559479934960571No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC93690.24436734444762653No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATCAATATC93590.2441065190186078No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAA87510.22824833293427044No Hit
TCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT87240.2275441042759199No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAATAT86580.22582265644439645No Hit
TTGTTGTTTTCTGATGTCTAACCTAGAACCATGCTGTAGGCACCATCAAT85540.22311007198260188No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCTGTAGGCACCAT84650.2207887256643354No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT84070.21927593817602692No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC82090.2141115946814565No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC79620.20766920658469445No Hit
TGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC79350.20696497792634397No Hit
TTAGCTTTGGTGAAGCGAACCCGGCGAACTCTGTAGGCACCATCAATATC79240.20667806995442337No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAAT78770.20545219043803545No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTGTAGGCACCATCAAT76580.19974011354252577No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACTGTAGGCACCATCAATA74150.19340205561737117No Hit
TGCACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATA73360.1913415347281234No Hit
TCACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATAT71370.18615110869065113No Hit
AACACATGTAGCTAACTGGTCCTAATTGCTCTCTGTAGGCACCATCAATA69740.1818996541976462No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACTGTAGGCACCAT69210.18051727942384707No Hit
ACTTACTGGAGGTCCGAACCGATTAACGCTGTAGGCACCATCAATATCTC67130.17509211050025797No Hit
TTTTTCTGATGTCTAACCTAGAACCATGCTGTAGGCACCATCAATATCTC67020.1748052025283374No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT66450.17331849758293075No Hit
AGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATATCT66440.17329241504002887No Hit
ACTTACTGGAGGTCCGAACCGATTAACGTTGCTGTAGGCACCATCAATAT65850.1717535450088185No Hit
TAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAAT65690.1713362243223886No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACTGTAGGCACCATCA65370.17050158294952872No Hit
GGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA63000.16432002028178538No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGATTGCTGTAGGCACCATCA62430.16283331533637874No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATATCT61510.16043372138940662No Hit
TAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATC61070.15928608950172432No Hit
GTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCG60360.1574342289556915No Hit
TTGTTGTTTTCTGATGTCTAACCTAGAACCACTGTAGGCACCATCAATAT60310.15730381624118214No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACC60010.15652133995412604No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACCAT59530.15526937789483625No Hit
ACACATGTAGCTAACTGGTCCTAATTGCTCTCTGTAGGCACCATCAATAT59310.15469556195099507No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAAT59150.15427824126456516No Hit
AAGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAATA58870.15354793006331277No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC58810.15339143480590153No Hit
AAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCA57050.1488009072551723No Hit
ACCTGCGGCTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTC55920.14585357990726092No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG54470.14207161118648964No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC54160.14126305235653167No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC53390.13925469655308764No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA52110.13591613106164818Illumina PCR Primer Index 6 (96% over 29bp)
AAGACATGCACCGAAGCTTAGGATTGTTACCTGTAGGCACCATCAATATC51460.13422076577302658No Hit
TAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT49740.12973456839390482No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG49710.1296563207651992No Hit
TTGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATC48830.12736105698983458No Hit
TTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA48640.12686548867469905No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA48010.1252222884718812No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT47360.12352692318325961No Hit
CTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC46030.12005794497731079No Hit
CGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGTA45470.11859732257480603No Hit
GGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAATATCT45280.11810175425967048No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCGCTGTAGGCACCATCAAT43380.11314607110831507No Hit
GGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCTC42400.11058998190393173No Hit
AACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAA42360.11048565173232426No Hit
TTGCACAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAAT41440.10808605778535214No Hit
GTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAAT40760.10631244486802494No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCAA40620.10594728926739876No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT39370.10268697140466491No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATCTC38680.10088727594443583No Hit
TGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCG38680.10088727594443583No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)