FastQCFastQC Report
Tue 18 Jul 2023
SRR10401012_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10401012_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3504534
Total Bases178.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC38028810.851314325956032No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT2032305.799059161646028No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG1459914.165774964660066No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA514401.467812839025103No Hit
TAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATAT514261.4674133565261458No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG439491.2540611676188618No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC415491.1855784535119362No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA398011.1357002100707254No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT373041.0644496529353118No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATAT342760.9780472953037408No Hit
GTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT323830.9240315545519033No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT305350.8712998646895707No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA278480.7946277593540253No Hit
TTTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATC250750.7155016900963153No Hit
TTCACGCTTTTGAGAACTCCACTGTCAACTGTAGGCACCATCAATATCTC240440.6860826574945484No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG237920.6788919725133213No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC236600.6751254232374404No Hit
TTTGGACAATGGGGGAAACCCTGATCCAGCCTGTAGGCACCATCAATATC233700.6668504286161869No Hit
ATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCTC232900.6645676714792894No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT214510.6120927917948578No Hit
CTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC191040.5451224042911268No Hit
TTCACGCTTTTGAGAACTCCACTGTCACTGTAGGCACCATCAATATCTCG175110.4996670028026551No Hit
TAGTCGAACATATCGTTAGATAGTTTGACCTGTAGGCACCATCAATATCT164700.4699626255587761No Hit
TTTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTC155840.44468109026763614No Hit
TTTGGACAATGGGGGAAACCCTGATCCAGCTGTAGGCACCATCAATATCT153920.43920247313908206No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG152250.43443721761580856No Hit
TTTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCT148920.4249352410334727No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGACTGTAGGCACCATCAATATCTCG145060.4139209378479421No Hit
AGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATATC143570.4096693026804705No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA143370.40909861339624615No Hit
TTCACGCTTTTGAGAACTCCACTGTCAATCTGTAGGCACCATCAATATCT142170.4056744776908998No Hit
ACTGCCTTTGAGACTGTTAGACTAGCTGTAGGCACCATCAATATCTCGTA138450.39505965700432644No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT135540.3867561279188617No Hit
TATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT134150.38278983739350225No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT132360.37768216829969403No Hit
TACTCTGGAACTGCCTTTGAGACTGTTAGACCTGTAGGCACCATCAATAT127790.364641918155167No Hit
ACCCACCCGATCCCATTTCGAACTCGGACGTGCTGTAGGCACCATCAATA117340.3348234030544432No Hit
TGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCGT111430.3179595347056128No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCCTGTAGGCACCATCAA111370.3177883279203455No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAAT103380.2949892910155815No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC102460.2923641203081494No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC102220.29167929316708013No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAATA101280.28899705353122557No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT101020.28825515746173386No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAAT98470.280978869087873No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAA95130.2714483580413259No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA93750.2675106019801777No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGCTGTAGGCACCATCAATATCTCG93550.2669399126959533No Hit
ACAGCTAAGGTCCCCAAGTCATGTCCTGTAGGCACCATCAATATCTCGTA92510.26397232841798657No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTCTGTAGGCACCATCA90820.25915000396629057No Hit
GTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC88330.25204492237769704No Hit
TATAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAAT88010.251131819522938No Hit
GATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT83980.23963243044581675No Hit
TTGAGTGAAGAAGGCCTTAGGGTTGTAAAGCTCCTGTAGGCACCATCAAT82880.23649363938258267No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGACTGTAGGCACCATCAATATCTC77650.22157011460011516No Hit
ACCTACAGCTAAGGTCCCCAAGTCATGTCCTGTAGGCACCATCAATATCT75010.21403701604835335No Hit
AGTAGGAGGGTCTTTAAGGCCTGACTGCGCTGTAGGCACCATCAATATCT72890.20798770963557492No Hit
ACCCACCCGATCCCATTTCGAACTCGGACCTGTAGGCACCATCAATATCT69220.19751556127005757No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAAT68360.19506159734789277No Hit
ACCCGATCCCATTTCGAACTCGGACGCTGTAGGCACCATCAATATCTCGT67160.1916374616425465No Hit
AAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT63980.18256350202337884No Hit
TGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATC62780.1791393663180326No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGACTGTAGGCACCATCAATA60570.1728332497273532No Hit
TACCCGATCCCATTTCGAACTCGGACGCTGTAGGCACCATCAATATCTCG58130.1658708404598158No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGCTGTAGGCACCATCAATATCTCGT56530.16130532618602073No Hit
ACTCTGGAACTGCCTTTGAGACTGTTAGACCTGTAGGCACCATCAATATC56050.15993567190388222No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGCTGTAGGCACCATCAATAT55590.15862308655016616No Hit
TACTCTGGAACTGCCTTTGAGACTGTTAGACTCTGTAGGCACCATCAATA53200.15180334960368483No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAAT52640.15020541960785655No Hit
ACTGCCTTTGAGACTGTTAGACTAGAACCTGTAGGCACCATCAATATCTC50460.14398490640981082No Hit
ACGGGGATATTATTGATGAAATGATATCCCCTGCCTGTAGGCACCATCAA50230.1433286137329528No Hit
TTGCCTTTGAGACTGTTAGACTAGAACATAGCTGTAGGCACCATCAATAT50000.14267232105609476No Hit
TATTCACGCTTTTGAGAACTCCACTGTCACTGTAGGCACCATCAATATCT49470.14115999445290017No Hit
TACTCTGGAACTGCCTTTGAGACTGTTAGCTGTAGGCACCATCAATATCT49080.14004715034866264No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGATCTGTAGGCACCATCAA48980.13976180570655042No Hit
TATTCACGCTTTTGAGAACTCCACTGTCAACTGTAGGCACCATCAATATC48450.13824947910335583No Hit
ACCCTGCCAATGCACGTCCCCTGTCATTGGCAGAGCTGTAGGCACCATCA46250.13197189697688766No Hit
TTAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCA45940.13108732858633987No Hit
ATTCACGCTTTTGAGAACTCCACTGTCACTGTAGGCACCATCAATATCTC45840.13080198394422768No Hit
GGAGGTTGGCTTAGAAGCAGCCATCCTTCTGTAGGCACCATCAATATCTC45250.12911845055576576No Hit
TTAAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCT45250.12911845055576576No Hit
TTGTATCGGGTCGTTCCGGCTTCCACGCTGTAGGCACCATCAATATCTCG44300.12640767645569995No Hit
TGTCTGACTGGCTTAGGTTAGTTAGACTGTAGGCACCATCAATATCTCGT44100.12583698717147557No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC43710.12472414306723804No Hit
AGGATGAGTCCTTCTTTGTAAAAAGGAGGACTGTCCTCTGTAGGCACCAT43590.12438172949670342No Hit
ATTCACGCTTTTGAGAACTCCACTGTCAACTGTAGGCACCATCAATATCT42470.1211858695050469No Hit
ATTGGACAATGGGGGAAACCCTGATCCAGCTGTAGGCACCATCAATATCT40440.11539337327016945No Hit
AACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCATC40020.11419492577329825No Hit
ATGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC39490.11268259917010365No Hit
TTTAAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATC38530.10994329060582662No Hit
ACCCACCCGATCCCATTTCGAACTCGGACGCTGTAGGCACCATCAATATC38390.10954380810686956No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT38230.10908725667949007No Hit
ACCCACCCGATCCCATTTCGAACTCGGACTGTAGGCACCATCAATATCTC38170.10891604989422274No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT37200.1061482068657345No Hit
ATAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATA36740.10483562151201843No Hit
TTGCCTTTGAGACTGTTAGACTAGAACACTGTAGGCACCATCAATATCTC35700.10186803723405166No Hit
AGTACCGGGAGAATAAGCTCCGGCTAACTCCGCTGTAGGCACCATCAATA35670.101782433841418No Hit
TTCTCTTAGCTCTTTTGAATATCTTCGCTGTAGGCACCATCAATATCTCG35590.10155415812772826No Hit
TACCCTGCCAATGCACGTCCCCTGTCATTGGCAGAGCTGTAGGCACCATC35130.10024157277401219No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)