FastQCFastQC Report
Tue 18 Jul 2023
SRR10401013_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10401013_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3606701
Total Bases183.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT1446494.0105625611881885No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA1364123.7821821104660467No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC987852.7389295647185614No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC773352.14420324834246No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC749012.0767177539807156No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC694831.926497372529633No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT582031.6137461907710122No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT533711.4797733441169645No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT494191.3701995258270645No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC411101.139822790966038No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCACTGTAGGCACCAT379971.0535112281278654No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA376471.0438070691193975No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT371681.0305262343620942No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA340720.9446860163900472No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA333320.9241686516292866No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCATCTGTAGGCACCA317670.8807771977771377No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG313340.868771766775233No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT301490.8359162569894205No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT282430.7830701796461642No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG257950.7151965189240805No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT250600.694817785006298No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT226450.6278590878478698No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA225880.6262786962379194No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA206340.5721017628020731No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC205930.5709649898896526No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC196920.5459837119849968No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA176520.4894223280499271No Hit
AAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC152240.42210319069975577No Hit
ATGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC151960.42132685797907843No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT135730.3763272863483832No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATC126160.3497933430023725No Hit
TTAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCAT120210.3332962726879772No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA119040.3300523109622894No Hit
TAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATAT116420.3227880547902363No Hit
AGGGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATC108440.3006625722509296No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA99790.2766794364157162No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATC93670.25971102123519524No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA93380.2589069623459222No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA93200.258407891311201No Hit
AGTAGGAGGGTCTTTAAGGCCTGACTGCGCTGTAGGCACCATCAATATCT93110.2581583557938404No Hit
ACTGCTTGGTGGGGCTTGCTCTGCTAGGCGGCTGTAGGCACCATCAATAT91320.253195371615224No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCCTGTAGGCACCATCAA90460.25081092111600045No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCAA88870.2464024603092965No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA88070.24418436682164668No Hit
TGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCGT87420.24238216586293126No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA86490.23980363218353834No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATAT83200.2306817227155786No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC79900.2215320870790232No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG79760.2211439207186845No Hit
AAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT78380.21731770945248857No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG75570.20952665607711865No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCACTGTAGGCACC75570.20952665607711865No Hit
TGACGGAAACACTCCTTTTCAAAGGGGTGTTTCTTCTGTAGGCACCATCA74900.20766900278121198No Hit
CAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC73200.20295555411995614No Hit
AGGATGAAGGTAAGGTAACACTTACTGGAGGTCCGCTGTAGGCACCATCA73010.20242875691663934No Hit
GTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT72380.2006820082951151No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATAT70820.196356725994198No Hit
TGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC70210.19466542970986506No Hit
TCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT68000.1885379464502325No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT65150.1806359884004801No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATCTC63540.17617207525658488No Hit
TTTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATAT62510.17331627989123577No Hit
AACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATCA62490.17326082755404454No Hit
TAGTCGAACATATCGTTAGATAGTTTGACCTGTAGGCACCATCAATATCT61370.17015549667133484No Hit
TTGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATC61170.16960097329942236No Hit
TTGACGGAAACACTCCTTTTCAAAGGGGTGTTTCTTCTGTAGGCACCATC60620.16807603402666313No Hit
TTAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCA59980.1663015592365433No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC58880.16325168069102486No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCTGTAGGCACCAT58840.16314077601664237No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC58800.16302987134225985No Hit
ATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCTC55740.1545456637519994No Hit
GAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA54760.1518284992296284No Hit
TGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATCT54360.1507194524858035No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCCTGTAGGCACCA53740.14900043003287491No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAAT53720.14894497769568366No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCA52380.14522967110387025No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTTCTGTAGGCACCATC52080.1443978860460016No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGACTGTAGGCACCATCAATATCTCG52010.14420380286583223No Hit
GGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCTC49190.1363850233218667No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA48920.1356364167697849No Hit
ACTCCGTCAATTGTGGTTCGAATCCACACGGGGCCTGTAGGCACCATCAA48020.1331410615961789No Hit
AACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCATC47830.13261426439286206No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAA47570.1318933840093759No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATCAATATC47000.1303129923994254No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCA46250.12823352975475372No Hit
ACATCCTGGGGCTGGAGCAGGTCCCAAGGGTTTGGCTGCTGTAGGCACCA45930.1273462923596938No Hit
CGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGTA45880.12720766151671567No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGCTGTAGGCACCATCAAT45680.12665313814480325No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT45250.12546091289519148No Hit
TTAACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATC45060.12493411569187467No Hit
ATAACCCCCAAGAGCTCATATCGACGGGGTTGCTGTAGGCACCATCAATA42870.11886208476943334No Hit
GGGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCAT41510.11509132584042868No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC40420.11206917346350585No Hit
CTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC39910.1106551388651291No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC37970.10527626215757835No Hit
CAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT37510.10400085840217971No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC36980.10253137146661172No Hit
CTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC36250.1005073611591313No Hit

[FAIL]Adapter Content

Adapter graph