FastQCFastQC Report
Tue 18 Jul 2023
SRR10401015_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10401015_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3165100
Total Bases161.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT2298227.261129190230957No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT1947636.153454867144799No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA1598545.050519730814193No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC872992.7581750971533285No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC845292.6706581150674547No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA699312.2094404600170607No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT564911.7848093267195348No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC452351.4291807525828568No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA445001.4059587374806484No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC422451.3347129632555053No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA349771.1050835676597897No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA338591.0697608290417364No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT305200.9642665318631323No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT301740.9533348077469906No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC296820.9377902751887777No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT283920.897033269091024No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC279160.8819942497867366No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA268210.8473981864712016No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA254930.8054405863953746No Hit
AGTACCGGGAGAATAAGCTCCGGCTAACTCCGCTGTAGGCACCATCAATA222520.7030425578970649No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA219470.6934062114941076No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC189800.5996650974692742No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCA174870.5524943919623392No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG172610.5453540172506398No Hit
TGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC159800.5048813623582193No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA144940.4579318188998768No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA140900.44516760923825466No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC140710.444567312249218No Hit
TTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA132510.4186597579855297No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC130250.41151938327383025No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATAT123980.3917095826356198No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC123910.39148842058702726No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT118910.3756911314018514No Hit
TCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT115960.3663707307825977No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG102120.32264383431803106No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC95970.30321316862026476No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA95100.30046444030204417Illumina PCR Primer Index 6 (96% over 29bp)
ACTGCTTGGTGGGGCTTGCTCTGCTAGGCGGCTGTAGGCACCATCAATAT93200.29446147041167736No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT91850.29019620233167986No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA91410.2888060408833844No Hit
GTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT82560.2608448390256232No Hit
CTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC82390.26030773119332723No Hit
TGGACCTGTCGTGGCGCCAGCCGCGCAGCAGGGCTGTAGGCACCATCAAT82290.2599917854096237No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCAATATCTC82280.2599601908312534No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATCAATATC81610.2578433540804398No Hit
AGTACCGGGAGAATAAGCTCCGGCTAACTCCGTGCTGTAGGCACCATCAA77210.24394173959748508No Hit
TAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT76820.24270955104104133No Hit
AAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT74100.2341158257243057No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAAT73420.2319673943951218No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCAA70180.22173075100312784No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT68180.21541183532905753No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT65700.20757637989321032No Hit
GTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAAT64770.20463808410476764No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGCTGTAGGCACCATCAAT64500.20378503048876814No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG64350.20331111181321285No Hit
ATGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC63970.2021105178351395No Hit
GTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCG63730.20135224795425105No Hit
GGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTCG62950.1988878708413636No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC60480.19108400998388678No Hit
CGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGTA60230.19029414552462798No Hit
TTTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATAT60000.1895674702221099No Hit
CCGCCCGTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAA59530.18808252503870337No Hit
GGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTC56910.17980474550567122No Hit
TCAGCTCGTGTCGTGAGATGTTGGGTTCTGTAGGCACCATCAATATCTCG56700.17914125935989386No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAATAT54750.1729803165776753No Hit
TTGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT54390.17184291175634261No Hit
GGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT54260.17143218223752804No Hit
TGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTC51740.16347034848819944No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG51500.162712078607311No Hit
TAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATC51430.1624909165587185No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCCTGTAGGCACCA50370.15914189125146125No Hit
TTCAGCTCGTGTCGTGAGATGTTGGGTTCTGTAGGCACCATCAATATCTC49620.15677229787368488No Hit
CCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT47200.14912640990805978No Hit
GGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA46320.14634608701146884No Hit
TTGAGGTAATCACGCCGGAGGATTATCTCGCTGTAGGCACCATCAATATC45960.14520868219013616No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA43520.13749960506777037No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT41740.13187577011784776No Hit
TGTTGTTTTCTGATGTCTAACCTAGAACCACTGTAGGCACCATCAATATC39620.12517771950333323No Hit
CGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT39230.12394553094688951No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT39070.12344001769296388No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCGTAT38930.12299769359577896No Hit
CGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA37920.11980664118037346No Hit
CTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC37460.11835329057533726No Hit
GAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC37120.11727907491074531No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAA36060.11393004960348804No Hit
CTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT35850.11326656345771065No Hit
TAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGCTGTAGGCACCATCAA34950.11042305140437901No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATC34650.10947521405326845No Hit
TGTTGTTTTCTGATGTCTAACCTAGAACCATGCTGTAGGCACCATCAATA34640.10944361947489811No Hit
TTTTTCTGATGTCTAACCTAGAACCATGCTGTAGGCACCATCAATATCTC34330.10846418754541721No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC33750.10663170199993681No Hit
ACCGGGAGAATAAGCTCCGGCTAACTCCGTGCTGTAGGCACCATCAATAT32990.10423051404379008No Hit
TTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAAT32960.10413573030867904No Hit
TTCGCCAATTAAAGTGGTACGTGAGCTGGGTTCTGTAGGCACCATCAATA31670.10006002969890365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)