FastQCFastQC Report
Tue 18 Jul 2023
SRR10401017_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10401017_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4604198
Total Bases234.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2152954.676058675148202Illumina PCR Primer Index 6 (96% over 29bp)
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT1885054.094198381563955No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA1296172.81519170113883No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC1292932.8081546449566246No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA1063542.309935411118288No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA1011542.1969950032557244No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC801651.7411284223658496No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC727791.5807096045826006No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT684921.4875989260235984No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT671351.45812582343331No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT563241.2233183716251994No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT561621.2197998435340964No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT411700.8941839599426438No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA372880.809869601611399No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT346110.751727010871383No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG316120.6865908025675699No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA314410.6828768006936279No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCACTGTAGGCACCAT300610.6529041539916398No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC300320.6522742940247139No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT295800.6424571662643527No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA285120.6192609440341185No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA273950.5950004756528715No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC231920.5037142190670341No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG231050.5018246391662565No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT218440.47443659025958484No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC192340.41774919323625964No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC188120.40858364475202846No Hit
ATGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC181270.3937059179470561No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT177420.38534398390338553No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC163370.354828354471289No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA156960.3409062772713076No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATC156340.3395596801006386No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA142920.3104123671484154No Hit
TTAACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATC142520.30954359478024185No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT141500.30732822524139924No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCAA141460.3072413480045819No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC141230.30674180389288214No Hit
ACTGCTTGGTGGGGCTTGCTCTGCTAGGCGGCTGTAGGCACCATCAATAT137040.297641413336264No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA134010.29106046264734925No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCACTGTAGGCACC123730.26873301278528855No Hit
TGGATGAAGGTAAGGTAACACTTACTGGAGGTCCGCTGTAGGCACCATCA121770.26447602818123805No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC117860.2559837782823415No Hit
GTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT112920.2452544395353979No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT111740.24269156104928588No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG111500.24217029762838177No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA111130.24136668318782123No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAA110850.2407585425300997No Hit
TGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCGT108290.23519839937378886No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATAT108150.23489432904492813No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATC105090.22824822042840034No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAATA102590.22281839312731558No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC102160.22188446283152896No Hit
ACATCCTGGGGCTGGAGCAGGTCCCAAGGGTTTGGCTGCTGTAGGCACCA101290.21999488293075148No Hit
TCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT98150.21317501984058898No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC92910.20179410181751525No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATC92870.2017072245806979No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAAT92600.2011208032321807No Hit
TGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC91730.19923122333140322No Hit
GGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCTC87170.18932721833422456No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCCTGTAGGCACCATCAA87050.18906658662377246No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC87020.18900142869615946No Hit
TTGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATC85890.18654714675606915No Hit
TTTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATAT85460.18561321646028253No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT84940.1844838123816569No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA82760.17974900297511096No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA79600.1728857012665398No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG78610.17073548965531024No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATCAATATC78070.1695626469582759No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT72130.15666137729089843No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC71790.1559229207779509No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT70830.15383786709433434No Hit
GGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA70820.15381614778513No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATCTC67810.14727863571462393No Hit
GGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTCG64520.14013298298639634No Hit
TAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATAT62550.13585427907314152No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCA62490.1357239632179155No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCTGTAGGCACCAT61480.13353031298827722No Hit
GGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTC61290.13311764611339477No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATAT58960.12805704706878374No Hit
ATAACCCCCAAGAGCTCATATCGACGGGGTTGCTGTAGGCACCATCAATA58050.1260805899311889No Hit
TGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATCT58020.12601543200357587No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCATCTGTAGGCACCA55840.12128062259702992No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAATATC54950.11934760407784374No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCCTGTAGGCACCA54220.11776209450592699No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT54200.11771865588751831No Hit
CTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC54090.11747974348627058No Hit
GTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCG53070.11526437394742799No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTTCTGTAGGCACCATC53040.11519921601981496No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAAT52450.11391777677675895No Hit
TCTGCTTGGTGGGGCTTGCTCTGCTAGGCGGCTGTAGGCACCATCAATAT52320.11363542575710255No Hit
GAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA52260.1135051099018765No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCA51670.1122236706588205No Hit
AAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT49660.10785808950874833No Hit
TGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCT49190.10683728197614438No Hit
AAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCA49030.10648977302887495No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA48460.10525177240422762No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA48210.10470878967411915No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCA48200.1046870703649148No Hit
TAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT48170.10462191243730179No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA47710.10362282421390219No Hit
TGGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCT47330.10279749046413729No Hit
GGGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCAT47240.10260201668129823No Hit
TAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATC47040.10216763049721146No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGACTGTAGGCACCATCAATATCTCG46210.1003649278332513No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)