FastQCFastQC Report
Tue 18 Jul 2023
SRR10401018_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10401018_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4594214
Total Bases234.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2119974.614434590987707Illumina PCR Primer Index 6 (96% over 29bp)
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT1872314.075365231136382No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA1298812.82705594471655No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC1297892.825053425896138No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA1063832.3155865181726405No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA994402.1644616467582924No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC801981.7456304821673523No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC728541.5857772406770776No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT688501.4986241389713235No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT670021.4583996304917446No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT556141.210522626939015No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT555191.2084548085918505No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT411910.8965842688216091No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA370160.8057090940909587No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT344890.7507051260563831No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG317050.6901071652300046No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA310080.6749359085144924No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCACTGTAGGCACCAT302150.6576750669428982No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT295640.643505069637592No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC295600.6434180036019219No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA285180.6207373013098649No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA272750.5936815307253863No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC229890.5003902735048912No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG228200.4967117334978301No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT216200.47059192279680484No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC189320.4120835468265083No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC185250.40322457769707726No Hit
ATGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC179210.39007760631089455No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT178620.3887933822847608No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC161020.3504843265899238No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATC157680.34321431261147173No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA156090.3397534376935859No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA143280.31187053977024143No Hit
TTAACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATC142400.30995508698549956No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC141910.30888852804854106No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCAA141130.30719074035297445No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT140220.30520998804148003No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA134100.2918888845839571No Hit
ACTGCTTGGTGGGGCTTGCTCTGCTAGGCGGCTGTAGGCACCATCAATAT133480.2905393610310708No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCACTGTAGGCACC123320.26842458797086943No Hit
TGGATGAAGGTAAGGTAACACTTACTGGAGGTCCGCTGTAGGCACCATCA120980.2633312248841695No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG114560.249357126159121No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC114540.24931359314128596No Hit
GTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT113380.2467886781068535No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA112510.24489499183102922No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT112050.24389373242082324No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAA112040.24387196591190574No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATAT110160.23977986223541176No Hit
TGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCGT109920.23925746602139122No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATC103250.2247392045734047No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC102310.22269315273515775No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAATA100560.21888401367459154No Hit
ACATCCTGGGGCTGGAGCAGGTCCCAAGGGTTTGGCTGCTGTAGGCACCA99780.21718622597902493No Hit
TCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT97570.2123758275082528No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATC94670.2060635399221717No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC92980.20238499991511064No Hit
TGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC92240.20077427825521404No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAAT91760.19972948582717304No Hit
TTGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATC87430.19030458746588644No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCCTGTAGGCACCATCAA87290.18999985634104113No Hit
GGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCTC86670.18865033278815485No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC85930.1870396111282583No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT84140.18314340603202203No Hit
TTTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATAT84030.18290397443392928No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA83320.1813585523007853No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG78860.17165068932357092No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA78360.17056236387769486No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATCAATATC77350.1683639464770252No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT72870.1586125504819758No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT72480.15776365663419248No Hit
GGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA70990.15452044680548185No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC70650.15378038550228615No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATCTC67480.14688040217543197No Hit
GGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTCG64110.13954508867022739No Hit
TAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATAT62620.13630187884151676No Hit
GGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTC62280.13556181753832103No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCTGTAGGCACCAT61460.13377696380708431No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCA61460.13377696380708431No Hit
TGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATCT59910.13040315492486854No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATAT58360.12702934604265279No Hit
ATAACCCCCAAGAGCTCATATCGACGGGGTTGCTGTAGGCACCATCAATA56130.12217541455404558No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCATCTGTAGGCACCA55280.12032526129605631No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTTCTGTAGGCACCATC54580.11880160567182983No Hit
CTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC54440.11849687454698454No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAATATC54200.11797447833296403No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT54010.11756091466353114No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAAT53120.11562369536987176No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCCTGTAGGCACCA52900.11514483217368629No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCA52800.11492716708451109No Hit
GTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCG52750.11481833453992348No Hit
GAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA52740.11479656803100595No Hit
TCTGCTTGGTGGGGCTTGCTCTGCTAGGCGGCTGTAGGCACCATCAATAT52550.11438300436157306No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA49830.10846251393600732No Hit
AAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT48980.10661236067801806No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA48360.10526283712513174No Hit
TGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCT48350.10524107061621421No Hit
AAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCA47970.10441394327734843No Hit
TAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATC47890.10423981120600825No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA47800.10404391262575058No Hit
TAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT47400.10317325226904972No Hit
TGGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCT46970.10223729238559633No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCA46600.10143193155564803No Hit
GGGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCAT46400.10099660137729762No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)