FastQCFastQC Report
Tue 18 Jul 2023
SRR10401021_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10401021_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3759616
Total Bases191.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC1691604.499395682963367No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA1073732.85595656577693No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT931572.477832842503064No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC851772.265577122769985No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA773982.058667693721912No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC563111.4977859440964183No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT436671.1614750017023014No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC417191.1096611994416452No Hit
ATGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC354780.9436602035952608No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA349120.9286054746016615No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCACTGTAGGCACCAT348470.9268765746288982No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC332580.8846116199101185No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG317060.8433308082527577No Hit
GGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCTC295240.7852929660901539No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC295040.7847609968677652No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT290440.7725257047528258No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA283090.7529758358300422No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA232050.6172172902764538No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA218570.5813625646874574No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT207050.5507211374778701No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC202750.5392837991965137No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT199190.5298147470379954No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCTGTAGGCACCAT189670.5044930120522947No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATAT187100.4976572075446003No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACCA177660.4725482602478551No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT170990.454807086681193No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG167160.4446198760724499No Hit
TGGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCT165910.44129506843252075No Hit
ATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCTC156020.4149891903854011No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG144680.38482653547596346No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA143380.38136873553043715No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA138050.3671917557537791No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC121430.3229851133732807No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT120830.3213892057061146No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA114240.303860819828408No Hit
TTAGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATAT112650.29963166451041806No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA110650.2943119722865314No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCA109380.29093396772436336No Hit
TTTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATAT107910.2870239939398066No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCCTGTAGGCACCATCAA106220.2825288540106224No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC102140.2716766818738935No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCAA100060.2661442019610513No Hit
TTTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTC98240.26130328203731445No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT97890.2603723358981343No Hit
CTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC97000.2580050728585047No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA92380.24571658382132644No Hit
ACGATGATAACTAGCTGTCCGGGTACACTGTAGGCACCATCAATATCTCG90720.24130123927550046No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA89960.23927975623042352No Hit
TATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT89720.23864139316355712No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTCTGTAGGCACCATCA88100.2343324424622089No Hit
TAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATAT86680.23055546098324936No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATC85710.22797541025466433No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAA85560.22757643333787278No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG85480.2273636456489173No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT85350.22701786565436471No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCTGTAGGCACCAT83570.22228333957510557No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA82450.219304311929729No Hit
TTTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCT81990.21808078271823508No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT78950.20999485053792727No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC78880.20980866131009124No Hit
ACTTACTGGAGGTCCGAACCGATTAACGTTGCTGTAGGCACCATCAATAT78720.2093830859321803No Hit
TGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCT76230.20276006911344135No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT75460.20071198760724498No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT75450.20068538914612558No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT72570.19302503234372873No Hit
GGGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCAT69920.18597644014707884No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC69530.18493910016342094No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT68900.18326339711289663No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA68040.18097592945662536No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAA67540.17964600640065367No Hit
CTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC65420.17400713264333378No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC65420.17400713264333378No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT64030.17030994654773254No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAAT62040.16501685278496528No Hit
GATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT60790.16169204514503607No Hit
TGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCGT60480.16086749285033367No Hit
AGGGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATC59960.1594843728721231No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCCTGTAGGCACCA59900.15932478210540651No Hit
TAGTCGAACAGAGACGAAAGTCGGTCATAGTGCTGTAGGCACCATCAATA58850.156531943687866No Hit
AAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT58570.15578718677652187No Hit
GGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTC58100.15453705910390847No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCACTGTAGGCACC57350.15254217451995097No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC57190.15211659914204004No Hit
ACCCACCCGATCCCATTTCGAACTCGGACGTGCTGTAGGCACCATCAATA56710.15083987300830723No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATAT55970.14887158688546917No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAATA55760.14831301920196105No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATC53520.14235496391120794No Hit
GTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT52310.13913655011575649No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATATCT52300.13910995165463705No Hit
TCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT51180.13613092400926052No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACTGTAGGCACCATC48640.12937491488492442No Hit
TTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATATCTCG48580.1292153241182078No Hit
TTAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCA48540.12910893027373008No Hit
GGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCAT48160.1280981887511916No Hit
AACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCATC48140.12804499182895274No Hit
TTAACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATC46440.12352325343864905No Hit
GGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTCG45810.12184755038812474No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCTACTGTAGGCACCATCAA45790.12179435346588588No Hit
GAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA44830.11924090119842026No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCATCTGTAGGCACCA44460.11825675813700123No Hit
TCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTCG43980.11698003200326841No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAAT42790.11381481513005583No Hit
TGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATC42460.11293706591311453No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATCTC42340.11261788437968133No Hit
AGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATATCT41870.11136775670706794No Hit
ACCCGATCCCATTTCGAACTCGGACGCTGTAGGCACCATCAATATCTCGT41860.11134115824594853No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATC41520.11043681056788779No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCTACTGTAGGCACCATCAA40730.10833553213945255No Hit
TGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC40290.10716519985019747No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC39820.1059150721775841No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA37760.1004357891869808No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGGCTGTAGGCACCATCAATAT37610.10003681227018929No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)