FastQCFastQC Report
Tue 18 Jul 2023
SRR10401023_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10401023_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797894
Total Bases91.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT1009885.617016353578131No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA892264.962806483585795No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT880434.897007276291038No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC577793.2137044786845053No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC539523.0008443211891245No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA368282.0483966240501386No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA284631.5831300399244894No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC280151.5582119969252914No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA272301.5145498010450005No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT252391.4038091233409755No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC241281.3420146015282324No Hit
TTAACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATC217021.2070789490370402No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC215501.198624613019455No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA200441.1148599416873297No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA164470.9147925294817159No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA148540.8261888631921571No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT130080.7235131770838548No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT126000.7008199593524423No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT123280.6856911475315007No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC121010.6730652641368179No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT119870.666724512123629No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG117370.6528193541999695No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC116900.6502051845103215No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA104490.5811799805772755No Hit
CGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGTA92050.5119879147491454No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT91410.5084281943206885No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC86750.48250897995098707No Hit
GTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT79150.440237299863062No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA76680.42649900383448636No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT68510.38105694773996684No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC67290.374271230673221No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCCTGTAGGCACCA67200.37377064498796925No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA66400.3693209944523982No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC66210.36826420245020003No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATAT65320.3633139662293772No Hit
ATGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC65070.3619234504370113No Hit
ACTGCTTGGTGGGGCTTGCTCTGCTAGGCGGCTGTAGGCACCATCAATAT64470.358586212535333No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCACTGTAGGCACCAT63820.35497087147518147No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC63700.35430342389484587No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA61660.34295681502913966No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA61000.3392858533372935No Hit
TGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC59920.3332788251142726No Hit
TGGATGAAGGTAAGGTAACACTTACTGGAGGTCCGCTGTAGGCACCATCA55350.3078601964298229No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC53450.2972922764078416No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA53390.2969585526176738No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCA49050.27281919846220076No Hit
GTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAAT48650.27059437319441526No Hit
TGGACCTGTCGTGGCGCCAGCCGCGCAGCAGGGCTGTAGGCACCATCAAT48070.2673683765561262No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAAT48050.2672571352927369No Hit
TAACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCA46290.2574679041144806No Hit
GGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA46200.2569673184292289No Hit
TCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT43330.24100419713286766No Hit
TTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA43210.240336749552532No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATC40470.22509669646820113No Hit
AAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT38710.21530746528994482No Hit
GTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCG38180.21235957181012896No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG37240.20713123243083298No Hit
GGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTC36550.20329340884390293No Hit
GGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTCG36500.20301530568542972No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT36360.2022366168417048No Hit
TGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAATA36260.2016804105247584No Hit
TTTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATAT36170.20117982483950667No Hit
GGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCTC36020.20034551536408707No Hit
AACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAA32920.18310311953874922No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATCAATATC31460.17498250731133203No Hit
AGTACCGGGAGAATAAGCTCCGGCTAACTCCGCTGTAGGCACCATCAATA30170.1678074458227237No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAAT29830.165916344345106No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT29360.163302174655458No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT28480.15840755906632983No Hit
TAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT27290.15178870389466786No Hit
TCAGCTCGTGTCGTGAGATGTTGGGTTCTGTAGGCACCATCAATATCTCG27240.15151060073619468No Hit
AAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC26370.14667160577876115No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT25940.1442799186158917No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA25890.14400181545741852No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATC25740.14316750598199893No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCATCTGTAGGCACCA25590.14233319650657936No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCCTGTAGGCACCATCA24640.13704923649558873No Hit
GAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC24430.13588120323000133No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCGTAT24110.1341013430157729No Hit
GGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT24100.13404572238407828No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCAA22850.12709314342224848No Hit
GTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGTAT22620.12581386889327179No Hit
CTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAATATCTCG22590.12564700699818787No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAAT22450.12486831815446293No Hit
TTGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATC22320.12414524994243264No Hit
TGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCT22060.12269911351837205No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGCTGTAGGCACCATCAAT21970.1221985278331203No Hit
CTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC21680.12058552951397579No Hit
GGGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCAT21630.12030742635550261No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA21560.11991808193364015Illumina PCR Primer Index 6 (96% over 29bp)
TACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCGTA20710.11519032823959588No Hit
TGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTC20520.11413353623739775No Hit
TTAGCTAACTGGTCCTAATTGCTCTATTCACGCTCTGTAGGCACCATCAA20490.11396667434231385No Hit
TGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAATATC20290.11285426170842107No Hit
TTCAGCTCGTGTCGTGAGATGTTGGGTTCTGTAGGCACCATCAATATCTC20240.11257615854994789No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCAATATCTC20080.11168622844283367No Hit
TGGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCT19790.11007323012368916No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG19620.1091276793848803No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAA19620.1091276793848803No Hit
CCGCCCGTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAA19330.1075146810657358No Hit
TTGAGGTAATCACGCCGGAGGATTATCTCGCTGTAGGCACCATCAATATC19070.10606854464167521No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCAA18610.10350999558372184No Hit
TAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATC18590.10339875432033258No Hit
GGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAATATCT18310.10184137663288269No Hit
TTAGCTAACTGGTCCTAATTGCTCTATTCACGCCTGTAGGCACCATCAAT18290.10173013536949342No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)