FastQCFastQC Report
Tue 18 Jul 2023
SRR10401029_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10401029_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4852135
Total Bases247.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC1241302.5582552834989136No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC1240282.556153116102499No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT944581.9467306659851797No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC763281.5730807160147027No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA727871.500102532184286No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA672221.3854107521740429No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG591531.2191128235302604No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC491661.0132859040401803No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA451210.929920540133364No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCACTGTAGGCACCAT434750.8959973290108374No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG430850.8879596301421951No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT422470.8706888823167533No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA398260.820793320878335No Hit
TGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCGT382860.789054715089337No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT373420.7695993619303667No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATAT329750.6795977440858508No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT323250.6662015793047803No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC322550.6647589154052803No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG322400.6644497731411018No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT320080.6596683727884735No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCTGTAGGCACCAT313730.6465813502715815No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCCTGTAGGCACCATCAA244080.5030362922713403No Hit
ATGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC239500.49359714847175523No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT233470.4811696294517774No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA230430.4749043462310921No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA216260.4457007070083582No Hit
ATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCTC197700.407449504187332No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT192980.39772182760784686No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC181860.37480408109007685No Hit
ATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCGTATG178620.3681266081838201No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA173150.35685322028344224No Hit
TTTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTC171840.35415337784294953No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACCA169790.34992843356584263No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG162530.3349659479796007No Hit
GGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCTC156820.32319793245653716No Hit
GTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT150380.309925424581138No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTCTGTAGGCACCATCA148050.3051234147442311No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT145990.3008778609828457No Hit
TTAGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATAT140520.2896044730824678No Hit
TAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATAT140420.2893983782396821No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT138550.28554440467958947No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC138320.2850703865411824No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA137810.2840193028429753No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAA131220.27043765270339754No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT129220.2663157558476835No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT117600.24236753511598505No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA116140.23935855041131376Illumina PCR Primer Index 6 (96% over 29bp)
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA115870.23880209433579236No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCGTAT114880.23676175539221392No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCAA114330.2356282337568926No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC113810.23455654057440695No Hit
AGGTGGGAAGGATATTACCAAAGTGCTGTAGGCACCATCAATATCTCGTA113150.2331963146120213No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAAT112570.23200096452386426No Hit
ACCCACCCGATCCCATTTCGAACTCGGACGTGCTGTAGGCACCATCAATA108290.2231801052526362No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC106230.21893455149125077No Hit
TTTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCT103290.21287536311335115No Hit
TATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT102580.21141208972957265No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA99740.20555899619445875No Hit
TGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCG99590.2052498539302802No Hit
TGGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCT99260.20456974094908734No Hit
TACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCGTA98070.2021172123199375No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA93080.19183307966493102No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAATA92080.18977213123707398No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCA89850.18517621624295286No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT86980.1792612942550032No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT86150.17755070705988188No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCTGTAGGCACCAT85490.17619048109749627No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAATAT82850.17074957724795375No Hit
TTTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATAT80790.1665040234865683No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA80600.16611244328527544No Hit
AGGGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATC78980.1627737068321471No Hit
TGGATGAAGGTAAGGTAACACTTACTGGAGGTCCGCTGTAGGCACCATCA78500.1617844515867757No Hit
GATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT72260.14892413339694793No Hit
ATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGTATGC72000.14838828680570512No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATC72000.14838828680570512No Hit
ACCCGATCCCATTTCGAACTCGGACGCTGTAGGCACCATCAATATCTCGT71530.1474196410446123No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCTACTGTAGGCACCATCAA71260.14686318496909093No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCTACTGTAGGCACCATCAA70060.1443900468556625No Hit
AGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATATC66880.13783623085507718No Hit
AAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT66480.13701185148393438No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA66120.13626991004990588No Hit
AACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCATC66060.13614625314423445No Hit
CGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGTA64380.13268385978543468No Hit
TTTGGACAATGGGGGAAACCCTGATCCAGCTGTAGGCACCATCAATATCT63150.13014889321917053No Hit
TGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCT60620.12493469369669229No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC60320.12431640916833518No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT59530.12268825991032814No Hit
TACCCGATCCCATTTCGAACTCGGACGCTGTAGGCACCATCAATATCTCG58840.1212662054951068No Hit
TGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATC57610.11873123892884266No Hit
ACCCACCCGATCCCATTTCGAACTCGGACCTGTAGGCACCATCAATATCT57420.11833965872754984No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT56830.1171236991551142No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCATCTGTAGGCACCA55190.11374374373342869No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCTACTGTAGGCACCATCAAT55080.11351703940636441No Hit
AGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATATCT53370.10999281759472891No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAA51530.106200672487472No Hit
GGGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCAT51230.1055823879591149No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC50800.10469618013513639No Hit
CTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC50200.10345961107842219No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACTGTAGGCACCATC49840.10271766964439365No Hit
TTGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATC49490.1019963376946437No Hit
TAGTCGAACAGAGACGAAAGTCGGTCATAGTGCTGTAGGCACCATCAATA49140.10127500574489375No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGACTGTAGGCACCATCAATATCTCG49010.10100708244927233No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)