FastQCFastQC Report
Tue 18 Jul 2023
SRR10401030_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10401030_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4831874
Total Bases246.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC1240612.567554534741593No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC1233532.5529018347746653No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT937871.9410067398280668No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC760421.5737579249790041No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA725211.5008876473186181No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA665951.3782437207592748No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG589731.2204995411718105No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC488191.0103533328890613No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA448140.9274662377371595No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCACTGTAGGCACCAT435750.9018240127950355No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG427590.884936155206034No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT418760.8666616720551902No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA394610.816681064117152No Hit
TGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCGT379880.786195997660535No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT367570.7607193399496759No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATAT330960.6849516357421571No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT320770.6638625096598132No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG318660.6594956739352061No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCTGTAGGCACCAT316460.6549425750754262No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC315950.6538870839761136No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT312440.6466228217043739No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCCTGTAGGCACCATCAA243510.5039659560659073No Hit
ATGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC240390.4975088340465832No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT233370.4829803095031037No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA228270.4724253985099777No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA212610.44001561298990827No Hit
ATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCTC194850.4032596876491399No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT190060.39334634967716464No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC179780.37207096045964777No Hit
ATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCGTATG177540.3674350779842355No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA172320.35663181614421235No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACCA167950.3475877061363769No Hit
TTTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTC167210.3460562092471782No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG161610.3344665030586476No Hit
GGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCTC154080.3188824874158556No Hit
GTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT149000.30836896823054577No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTCTGTAGGCACCATCA147200.3046437055270895No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT146730.30367099804340925No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC141540.29292982391511035No Hit
TAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATAT141060.291936420527522No Hit
TTAGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATAT138500.28663826912705087No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA137960.285520690316014No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT136390.2822714334024438No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAA130030.26910883851689843No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT125550.25983707356607394No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA115670.2393895205048807No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT114510.2369887956515422No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA113940.23580912912878108Illumina PCR Primer Index 6 (96% over 29bp)
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC113710.23533312333889503No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCCTGTAGGCACCATCAA112560.23295309438946463No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAAT112160.23212525823314104No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCGTAT111750.2312767261729093No Hit
AGGTGGGAAGGATATTACCAAAGTGCTGTAGGCACCATCAATATCTCGTA110030.22771703070071778No Hit
ACCCACCCGATCCCATTTCGAACTCGGACGTGCTGTAGGCACCATCAATA107440.22235679158852237No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC105600.21854874526943377No Hit
TATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT103280.2137472955627568No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA101030.20909071718343647No Hit
TTTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCT100320.20762130800596207No Hit
TGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCG98780.20443413880411615No Hit
TGGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATCT97880.20257150745238806No Hit
TACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCGTA97810.20242663612503142No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA91840.1900711814919015No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAATA90940.18820855014017338No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCA88930.1840486734546472No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCTGTAGGCACCAT86770.1795783582104997No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT86480.17897817699716506No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT84690.1752736101976169No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAATAT82910.17158973930197682No Hit
TTTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATAT80100.16577419030380344No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA79310.1641392138950643No Hit
TGGATGAAGGTAAGGTAACACTTACTGGAGGTCCGCTGTAGGCACCATCA77840.16109691602057505No Hit
AGGGCGATTTAGTTTGATGCATTAGACCCGCTGTAGGCACCATCAATATC77530.16045534299942424No Hit
ATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGTATGC73040.1511628821446917No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATC73030.1511421862407836No Hit
GATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT72300.149631385255493No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCTACTGTAGGCACCATCAA69630.14410557891203288No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCTACTGTAGGCACCATCAA69080.14296730419708792No Hit
ACCCGATCCCATTTCGAACTCGGACGCTGTAGGCACCATCAATATCTCGT68510.14178763767432676No Hit
AGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATATC66590.13781402412397342No Hit
AAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT66330.13727593062236307No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA65530.13562025830971586No Hit
AACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCATC64340.1331574457446531No Hit
TTTGGACAATGGGGGAAACCCTGATCCAGCTGTAGGCACCATCAATATCT63030.1304462823326933No Hit
CGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGTA62540.12943218304119686No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC60130.12444447019934708No Hit
TGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCT60120.124423774295439No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT59610.12336828319612639No Hit
TACCCGATCCCATTTCGAACTCGGACGCTGTAGGCACCATCAATATCTCG58710.12150565184439825No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT57290.11856683348944942No Hit
ACCCACCCGATCCCATTTCGAACTCGGACCTGTAGGCACCATCAATATCT56180.1162695881556514No Hit
TGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATC55490.11484157078599318No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCATCTGTAGGCACCA54560.11291685172254078No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCTACTGTAGGCACCATCAAT53340.11039195144575377No Hit
AGCTTTGGTGAAGCGAACCCGGCGAACTGCTGTAGGCACCATCAATATCT52800.10927437263471688No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAA51880.10737034947517257No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC50710.104948928717926No Hit
GGGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCAT50180.10385204581079721No Hit
TTGTATGTCCTTATCGGATTGGACATGCTCCTGTAGGCACCATCAATATC49930.10333464821309496No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGACTGTAGGCACCATCAATATCTCG49880.1032311686935545No Hit
CTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC49190.10180315132389628No Hit
TAGTCGAACAGAGACGAAAGTCGGTCATAGTGCTGTAGGCACCATCAATA49140.10169967180435582No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)