FastQCFastQC Report
Tue 18 Jul 2023
SRR10401031_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10401031_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5741835
Total Bases292.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC3387635.8999083045751055No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC2199983.831492893822271No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC2154693.7526156707742384No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA1879593.273500544686498No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA1469422.559147032264076No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA1431292.492739690360312No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT1273992.2187854579589974No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT1104021.922765109063566No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT1033131.7993028361142387No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG950671.6556902105337403No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA743981.2957181806861395No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA632041.1007630835786817No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT627831.0934309327941327No Hit
ATGGGGAAACCCATCCTCACCATTTAATTCTGTAGGCACCATCAATATCT588231.0244634337280678No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC566670.9869144620143213No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAAT557820.9715012709351626No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT424590.7394674350621361No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC385930.6721370432971341No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCACTGTAGGCACCAT379340.6606598761545743No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGATATTGCTGTAGGCACCA335290.5839422414611357No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC325980.5677279127665633No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA305810.532599769934176No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAAT289390.5040026402709239No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT280650.48878102557806No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG270270.47070318112589443No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT269290.46899640968436046No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC269010.4685087607010651No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA259700.45229443200649266No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT254530.44329034185064525No Hit
GTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT252460.43968522258128284No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA247110.43036764379331693No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT228190.3974165053506414No Hit
ACGATGATAACTAGCTGTCCGGGTACACTGTAGGCACCATCAATATCTCG226060.39370688987057273No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATAT210340.366328882665559No Hit
TTGAGTGAAGAAGGCCTTAGGGTTGTAAAGCTCCTGTAGGCACCATCAAT203590.3545730589611161No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACCA201430.3508111953756944No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT200290.34882576737227733No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA174090.3031957553639211No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCCTGTAGGCACCA173070.3014193197819164No Hit
TGGATGAAGGTAAGGTAACACTTACTGGAGGTCCGCTGTAGGCACCATCA170610.2971349751429639No Hit
ATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCTC167630.2919449966778913No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC164890.28717300305564336No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA158030.27522560296490584No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCATCTGTAGGCACCA157970.27512110675419965No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGATATTGCTGTAGGCACCA157560.2744070493143742No Hit
TAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATAT151160.2632607868390506No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCCTGTAGGCACCATCAA149510.26038714104463123No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG146660.2554235710360886No Hit
GAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC146330.25484884187720475No Hit
AAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT141720.24682004968794818No Hit
TGGACCTGTCGTGGCGCCAGCCGCGCAGCAGGGCTGTAGGCACCATCAAT139020.24211772020617103No Hit
TAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT138750.24164748725799332No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA135900.23668391724945073No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA135330.23569120324774223No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC132100.23006582390472735No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA131790.22952592681607883No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCA131150.22841130056854647No Hit
TTTGGACAATGGGGGAAACCCTGATCCAGCTGTAGGCACCATCAATATCT129720.22592080754671634No Hit
TTTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATAT127410.22189770343452922No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATATCT126380.2201038518174068No Hit
AGCTGTCCGGGTACATGGTATCTGGGTGGCGGCTGTAGGCACCATCAATA118780.20686766512795995No Hit
CTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC112220.1954427460907532No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT112110.1952511697044586No Hit
GGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTCG112000.19505959331816397No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA109200.19018310348520986No Hit
TAACGATGATAACTAGCTGTCCGGGTCTGTAGGCACCATCAATATCTCGT109140.1900786072745037No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATAT109110.19002635916915062No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT108090.18824992358714593No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATC102580.17865368823729696No Hit
TAGTCGAACAGAGACGAAAGTCGGTCATAGTGCTGTAGGCACCATCAATA102520.1785491920265908No Hit
AGTACCGGGAGAATAAGCTCCGGCTAACTCCGCTGTAGGCACCATCAATA101730.17717332525229304No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC100950.17581487451311298No Hit
AGGATGAAGGTAAGGTAACACTTACTGGAGGTCCGCTGTAGGCACCATCA98500.17154794590927813No Hit
ACCGCCCGTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCA97580.16994567067845034No Hit
TTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATATCTCG97500.16980634239750883No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG97070.169057452887448No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAA95600.1664972957251471No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGATCTGTAGGCACCATCAA93700.1631882490527854No Hit
AGTAGGAGGGTCTTTAAGGCCTGACTGCGCTGTAGGCACCATCAATATCT90370.15738870935859356No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT88750.15456731166952725No Hit
CTGGGTTCAGAACGTCGCGAGACAGTTTGGTCCCTCTGTAGGCACCATCA88500.15413191079158492No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAAT88150.15352234956246566No Hit
CTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT88090.15341785335175948No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTCTGTAGGCACCATCA87680.1527037959119341No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCTGTAGGCACCAT86330.1503526311710455No Hit
CTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC85620.14911609267768927No Hit
TTTGGACAATGGGGGAAACCCTGATCCAGCCTGTAGGCACCATCAATATC85280.1485239474836877No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC85180.1483497871325108No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGCTGTAGGCACCATCAATATCTCGT85040.14810596264086306No Hit
GGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTC84210.14666043172609455No Hit
TTTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGCTGTAGGCACCATC83920.14615536670768142No Hit
ACTGCCTTTGAGACTGTTAGACTAGCTGTAGGCACCATCAATATCTCGTA83840.14601603842673988No Hit
TATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT82770.14415252266914672No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGACTGTAGGCACCATCAATATCTCG81880.14260249554367202No Hit
AGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTCTGTAGGCACCATCAAT80890.1408783080670204No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGCTGTAGGCACCATCAAT80440.14009458648672418No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA77960.13577540977753627No Hit
TTTCCGTTAGTAGTGGCGAGCGAACGCGGACTGTAGGCACCATCAATATC77800.1354967532156532No Hit
AAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC75910.13220512257840916No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCTGTAGGCACCAT75330.13119499254158296No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAA74220.12926181264351902No Hit
TGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCT72510.12628367063839346No Hit
TGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCGT71390.12433307470521182No Hit
CTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC71180.12396733796774027No Hit
TCGACTTACTGGTCTATAGTTGACGCTGAGGTACGCTGTAGGCACCATCA70190.12224315049108865No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATC70020.12194707789408786No Hit
GATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT69670.1213375166649686No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT67210.11705317202601608No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA67190.1170183399557807No Hit
AGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTTCTGTAGGCACCATCAA66190.11527673644401136No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGGCTGTAGGCACCATCAATAT65670.11437110261789131No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA65480.11404019795065515No Hit
TCTACGGCGAGATGTGAGCAATCTTTGACCCGTAGCTGTAGGCACCATCA64550.11242050668470967No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC63350.1103305824705865No Hit
TTGGGGAAACCCATCCTCACCATTTAATTCTGTAGGCACCATCAATATCT63270.11019125418964494No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATCTC63200.11006934194382109No Hit
ATGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC60880.10602882179651628No Hit
GAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA60440.10526251625133777No Hit
TAACGATGATAACTAGCTGTCCGGGTACACTGTAGGCACCATCAATATCT59910.10433946639010004No Hit
ATTCCGTTAGTAGTGGCGAGCGAACGCGGACTGTAGGCACCATCAATATC59250.10319000807233229No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)