FastQCFastQC Report
Tue 18 Jul 2023
SRR10401033_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10401033_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3509143
Total Bases178.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC38257310.902177540214234No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT2041555.817802238324286No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG1484444.230206634497369No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA519761.481159359991884No Hit
TAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATAT519401.4801334684850402No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG441511.2581704421848867No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC425031.2112074087604865No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA405171.154612393966276No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT375511.0700903325968762No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATAT350570.9990188487616493No Hit
GTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT329320.9384627528715701No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT308010.8777356750636836No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA285470.8135034679407479No Hit
TTTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATC254780.7260462169823231No Hit
TTCACGCTTTTGAGAACTCCACTGTCAACTGTAGGCACCATCAATATCTC242030.6897125594482755No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG241640.6886011769825282No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC238570.6798526021880557No Hit
TTTGGACAATGGGGGAAACCCTGATCCAGCCTGTAGGCACCATCAATATC237770.6775728432839585No Hit
ATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCTC234910.6694227052018114No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT216470.6168742624623733No Hit
CTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC195130.5560616936955832No Hit
TTCACGCTTTTGAGAACTCCACTGTCACTGTAGGCACCATCAATATCTCG178030.5073318471205078No Hit
TAGTCGAACATATCGTTAGATAGTTTGACCTGTAGGCACCATCAATATCT165750.47233754794261734No Hit
TTTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTC159270.45387150081943084No Hit
TTTGGACAATGGGGGAAACCCTGATCCAGCTGTAGGCACCATCAATATCT156200.44512292602495823No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG154050.43899607397019724No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGACTGTAGGCACCATCAATATCTCG148250.42246782191549326No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA147140.4193046564360586No Hit
TTCACGCTTTTGAGAACTCCACTGTCAATCTGTAGGCACCATCAATATCT147120.4192476624634562No Hit
TTTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCT147110.419219165477155No Hit
AGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATATC143510.40896025040871803No Hit
ACTGCCTTTGAGACTGTTAGACTAGCTGTAGGCACCATCAATATCTCGTA141200.4023774465731377No Hit
TATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT135940.38738803177869924No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT135410.3858776915047349No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT135090.38496578794309605No Hit
TACTCTGGAACTGCCTTTGAGACTGTTAGACCTGTAGGCACCATCAATAT129250.3683235479431873No Hit
ACCCACCCGATCCCATTTCGAACTCGGACGTGCTGTAGGCACCATCAATA116640.33238884821735676No Hit
TGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCGT115180.3282282882173796No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCCTGTAGGCACCATCAA110440.31472071671060425No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC105480.30058621150520226No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC104390.29748003999836997No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAAT104120.29671062136823717No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT103350.2945163534230437No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAATA100990.28779106465595733No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAAT99070.28231964328612424No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAA97060.2765917490395803No Hit
ACAGCTAAGGTCCCCAAGTCATGTCCTGTAGGCACCATCAATATCTCGTA96080.2737990443820614No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA94710.2698949572587951No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGCTGTAGGCACCATCAATATCTCG92840.2645660208204681No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTCTGTAGGCACCATCA91590.2610038975328164No Hit
GTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC89720.25567496109448945No Hit
TATAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAAT88650.2526257835602596No Hit
TTGAGTGAAGAAGGCCTTAGGGTTGTAAAGCTCCTGTAGGCACCATCAAT84540.24091352219046072No Hit
GATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT83210.2371234230123993No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGACTGTAGGCACCATCAATATCTC78440.22353036054672037No Hit
AGTAGGAGGGTCTTTAAGGCCTGACTGCGCTGTAGGCACCATCAATATCT73540.20956683725912567No Hit
ACCTACAGCTAAGGTCCCCAAGTCATGTCCTGTAGGCACCATCAATATCT72060.20534928328654603No Hit
ACCCACCCGATCCCATTTCGAACTCGGACCTGTAGGCACCATCAATATCT69110.196942672327688No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAAT68710.19580279287563945No Hit
ACCCGATCCCATTTCGAACTCGGACGCTGTAGGCACCATCAATATCTCGT66490.18947646191676998No Hit
AAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT63550.18109834794421314No Hit
TGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATC62300.17753622465656144No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGACTGTAGGCACCATCAATA60680.17291971287576482No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGCTGTAGGCACCATCAATATCTCGT58220.16590945424566625No Hit
TACCCGATCCCATTTCGAACTCGGACGCTGTAGGCACCATCAATATCTCG57610.16417113808129222No Hit
ACTCTGGAACTGCCTTTGAGACTGTTAGACCTGTAGGCACCATCAATATC57000.16243282191691816No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGCTGTAGGCACCATCAATAT55710.1587567106840616No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAAT52950.15089154246492661No Hit
TACTCTGGAACTGCCTTTGAGACTGTTAGACTCTGTAGGCACCATCAATA52170.14866877753343194No Hit
ACGGGGATATTATTGATGAAATGATATCCCCTGCCTGTAGGCACCATCAA50700.14447972054715355No Hit
TATTCACGCTTTTGAGAACTCCACTGTCACTGTAGGCACCATCAATATCT50670.1443942295882499No Hit
ACTGCCTTTGAGACTGTTAGACTAGAACCTGTAGGCACCATCAATATCTC50640.14430873862934626No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGATCTGTAGGCACCATCAA50050.14262741643757465No Hit
TTGCCTTTGAGACTGTTAGACTAGAACATAGCTGTAGGCACCATCAATAT49280.14043314849238117No Hit
TACTCTGGAACTGCCTTTGAGACTGTTAGCTGTAGGCACCATCAATATCT48600.13849535342389865No Hit
TATTCACGCTTTTGAGAACTCCACTGTCAACTGTAGGCACCATCAATATC47410.1351042120540542No Hit
TTAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCA46470.13242549534174014No Hit
ACCCTGCCAATGCACGTCCCCTGTCATTGGCAGAGCTGTAGGCACCATCA46030.13117162794448672No Hit
GGAGGTTGGCTTAGAAGCAGCCATCCTTCTGTAGGCACCATCAATATCTC45970.13100064602667946No Hit
ATTCACGCTTTTGAGAACTCCACTGTCACTGTAGGCACCATCAATATCTC45840.13063018520476366No Hit
TGTCTGACTGGCTTAGGTTAGTTAGACTGTAGGCACCATCAATATCTCGT45750.13037371232805273No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC45440.1294903057527151No Hit
TTAAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCT44660.12726754082122044No Hit
ATTCACGCTTTTGAGAACTCCACTGTCAACTGTAGGCACCATCAATATCT44520.12686858301300347No Hit
TTGTATCGGGTCGTTCCGGCTTCCACGCTGTAGGCACCATCAATATCTCG43860.12498778191712336No Hit
AGGATGAGTCCTTCTTTGTAAAAAGGAGGACTGTCCTCTGTAGGCACCAT43470.12387639945137602No Hit
ATGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC41490.11823399616373571No Hit
AACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCATC39970.11390245424595122No Hit
ATTGGACAATGGGGGAAACCCTGATCCAGCTGTAGGCACCATCAATATCT39910.11373147232814394No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT39060.11130922849254077No Hit
ACCCACCCGATCCCATTTCGAACTCGGACTGTAGGCACCATCAATATCTC38550.10985588219117888No Hit
ACCCACCCGATCCCATTTCGAACTCGGACGCTGTAGGCACCATCAATATC38190.10882999068433517No Hit
TTTAAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATC38040.10840253588981698No Hit
AGTACCGGGAGAATAAGCTCCGGCTAACTCCGCTGTAGGCACCATCAATA37210.10603728602681624No Hit
TTGCCTTTGAGACTGTTAGACTAGAACACTGTAGGCACCATCAATATCTC37140.10583780712270774No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT36860.10503989150627376No Hit
ATAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATA36180.10310209643779122No Hit
TACCCTGCCAATGCACGTCCCCTGTCATTGGCAGAGCTGTAGGCACCATC35760.10190522301314026No Hit
TTCTCTTAGCTCTTTTGAATATCTTCGCTGTAGGCACCATCAATATCTCG35160.10019540383506742No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)