FastQCFastQC Report
Tue 18 Jul 2023
SRR10401034_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10401034_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3519059
Total Bases179.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC38481110.93505394481877No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT2044365.809393931730044No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG1477854.199560166510422No Hit
TAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATAT522201.4839194227775097No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA522181.483862589402451No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG440091.2505900014748261No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC425761.2098688882454087No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA406561.1553088481892462No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT374681.0647164483459925No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATAT347960.9887870592678326No Hit
GTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT326620.9281458480804101No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT308570.8768537270901113No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA286470.8140528476504656No Hit
TTTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATC257400.7314455370029317No Hit
TTCACGCTTTTGAGAACTCCACTGTCAACTGTAGGCACCATCAATATCTC244470.6947027600276097No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG240380.6830803348281458No Hit
TTTGGACAATGGGGGAAACCCTGATCCAGCCTGTAGGCACCATCAATATC238760.6784768314484071No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC236610.6723672436296181No Hit
ATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCTC234370.6660019056230657No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT217050.6167842028224023No Hit
CTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC194160.5517384050679457No Hit
TTCACGCTTTTGAGAACTCCACTGTCACTGTAGGCACCATCAATATCTCG176370.5011851179534075No Hit
TAGTCGAACATATCGTTAGATAGTTTGACCTGTAGGCACCATCAATATCT168820.47973051886882256No Hit
TTTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTC157340.4471081615852419No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG156700.44528949358336983No Hit
TTTGGACAATGGGGGAAACCCTGATCCAGCTGTAGGCACCATCAATATCT156090.4435560756440855No Hit
TTCACGCTTTTGAGAACTCCACTGTCAATCTGTAGGCACCATCAATATCT147850.4201407251199824No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA147280.418520973930815No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGACTGTAGGCACCATCAATATCTCG147260.41846414055575654No Hit
TTTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCT147230.4183788904931688No Hit
AGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATATC144190.40974021748427636No Hit
ACTGCCTTTGAGACTGTTAGACTAGCTGTAGGCACCATCAATATCTCGTA139810.39729370834646427No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT136980.3892517857756861No Hit
TATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT133200.37851027788962904No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT131810.37456035832306306No Hit
TACTCTGGAACTGCCTTTGAGACTGTTAGACCTGTAGGCACCATCAATAT126920.3606645981212591No Hit
ACCCACCCGATCCCATTTCGAACTCGGACGTGCTGTAGGCACCATCAATA115260.32753074046215197No Hit
TGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCGT114120.32429123808381727No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCCTGTAGGCACCATCAA109900.31229939594647316No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAAT105820.3007053874345386No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT105110.29868780261996175No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC103550.2942547993653985No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAATA103270.2934591321145795No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC102180.2903617131738911No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAAT99540.2828597076661687No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA97050.2757839524713851No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAA96850.2752156187208001No Hit
ACAGCTAAGGTCCCCAAGTCATGTCCTGTAGGCACCATCAATATCTCGTA94790.26936178108977427No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGCTGTAGGCACCATCAATATCTCG94130.26748627971284367No Hit
GTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC91610.2603252744554723No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTCTGTAGGCACCATCA90720.25779618926536896No Hit
TATAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAAT89460.25421568663668326No Hit
TTGAGTGAAGAAGGCCTTAGGGTTGTAAAGCTCCTGTAGGCACCATCAAT83020.23591533986784535No Hit
GATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT82470.23435242205373652No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGACTGTAGGCACCATCAATATCTC79440.22574216573237335No Hit
ACCTACAGCTAAGGTCCCCAAGTCATGTCCTGTAGGCACCATCAATATCT75340.21409132384538027No Hit
AGTAGGAGGGTCTTTAAGGCCTGACTGCGCTGTAGGCACCATCAATATCT72250.20531056739884157No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAAT69280.1968708112026539No Hit
ACCCACCCGATCCCATTTCGAACTCGGACCTGTAGGCACCATCAATATCT68530.19473955963796005No Hit
ACCCGATCCCATTTCGAACTCGGACGCTGTAGGCACCATCAATATCTCGT67940.1930629750737342No Hit
TGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATC63610.18075854937356833No Hit
AAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT62500.1776042970578214No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGACTGTAGGCACCATCAATA60910.17308604374067044No Hit
TACCCGATCCCATTTCGAACTCGGACGCTGTAGGCACCATCAATATCTCG57870.164447370731778No Hit
ACTCTGGAACTGCCTTTGAGACTGTTAGACCTGTAGGCACCATCAATATC57150.1624013692296719No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGCTGTAGGCACCATCAATATCTCGT56550.1606963679779168No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGCTGTAGGCACCATCAATAT56100.1594176170391005No Hit
TACTCTGGAACTGCCTTTGAGACTGTTAGACTCTGTAGGCACCATCAATA52740.14986961002927202No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAAT51990.14773835846457817No Hit
TATTCACGCTTTTGAGAACTCCACTGTCACTGTAGGCACCATCAATATCT51210.14552185683729654No Hit
ACGGGGATATTATTGATGAAATGATATCCCCTGCCTGTAGGCACCATCAA50680.14401577239824623No Hit
ACTGCCTTTGAGACTGTTAGACTAGAACCTGTAGGCACCATCAATATCTC50640.14390210564812922No Hit
TTGCCTTTGAGACTGTTAGACTAGAACATAGCTGTAGGCACCATCAATAT50390.14319168845989794No Hit
TACTCTGGAACTGCCTTTGAGACTGTTAGCTGTAGGCACCATCAATATCT49680.1411741036453211No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGATCTGTAGGCACCATCAA49100.13952593576862451No Hit
TTAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCA47460.13486559901382728No Hit
ACCCTGCCAATGCACGTCCCCTGTCATTGGCAGAGCTGTAGGCACCATCA47350.1345530154510055No Hit
TATTCACGCTTTTGAGAACTCCACTGTCAACTGTAGGCACCATCAATATC47090.13381418157524497No Hit
ATTCACGCTTTTGAGAACTCCACTGTCACTGTAGGCACCATCAATATCTC46560.13230809713619465No Hit
AGGATGAGTCCTTCTTTGTAAAAAGGAGGACTGTCCTCTGTAGGCACCAT46140.13111459625996608No Hit
TGTCTGACTGGCTTAGGTTAGTTAGACTGTAGGCACCATCAATATCTCGT46100.13100092950984907No Hit
GGAGGTTGGCTTAGAAGCAGCCATCCTTCTGTAGGCACCATCAATATCTC45780.13009159550891303No Hit
TTAAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCT45530.12938117832068174No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC45450.12915384482044773No Hit
TTGTATCGGGTCGTTCCGGCTTCCACGCTGTAGGCACCATCAATATCTCG43760.12435142462800423No Hit
ATTCACGCTTTTGAGAACTCCACTGTCAACTGTAGGCACCATCAATATCT43080.12241908987601516No Hit
ATTGGACAATGGGGGAAACCCTGATCCAGCTGTAGGCACCATCAATATCT40010.11369516680453497No Hit
AACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCATC39990.11363833342947645No Hit
ATGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC39920.11343941661677169No Hit
ACCCACCCGATCCCATTTCGAACTCGGACGCTGTAGGCACCATCAATATC38070.10818232942386018No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT37830.10750032892315815No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT37710.10715932867280714No Hit
TTGCCTTTGAGACTGTTAGACTAGAACACTGTAGGCACCATCAATATCTC37310.10602266117163707No Hit
TTTAAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATC37290.10596582779657858No Hit
ATAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATA37020.10519857723328878No Hit
ACCCACCCGATCCCATTTCGAACTCGGACTGTAGGCACCATCAATATCTC37000.10514174385823029No Hit
AGTACCGGGAGAATAAGCTCCGGCTAACTCCGCTGTAGGCACCATCAATA35310.1003393236657868No Hit
TTCTCTTAGCTCTTTTGAATATCTTCGCTGTAGGCACCATCAATATCTCG35200.10002674010296503No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)