FastQCFastQC Report
Tue 18 Jul 2023
SRR10401035_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10401035_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3289246
Total Bases167.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC1724615.243177311760811No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC958272.913342449911013No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT801192.4357861953772995No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG703542.1389096467701108No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT690782.1001165616679325No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA668642.032806302721049No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA564531.716289994728275No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC525631.5980258089543926No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAAT476931.4499675609546991No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT429951.3071384748966786No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA373111.1343329139869744No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC360741.096725510952966No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT284890.8661255497460512No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAAT275750.8383380264048357No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT274250.8337777107580279No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA262270.7973559897921895No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT256280.7791451293092703No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT240680.731717846582469No Hit
ATGGGGAAACCCATCCTCACCATTTAATTCTGTAGGCACCATCAATATCT238820.7260630551804274No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA221190.6724641452782796No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG211070.6416972157144829No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCACTGTAGGCACCAT209430.6367112706073064No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATAT194290.5906824846788595No Hit
TTGAGTGAAGAAGGCCTTAGGGTTGTAAAGCTCCTGTAGGCACCATCAAT191530.5822915038887332No Hit
ACGATGATAACTAGCTGTCCGGGTACACTGTAGGCACCATCAATATCTCG190000.5776399819289891No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC178780.5435288208908668No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA167400.5089312261837515No Hit
ATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCTC166650.5066510683603477No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC148070.4501639585485549No Hit
GTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT146010.44390112506027213No Hit
AGTACCGGGAGAATAAGCTCCGGCTAACTCCGCTGTAGGCACCATCAATA140250.4263895129765302No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACCA137410.4177553153519074No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT133210.40498643154084557No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA129710.3943456950316273No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA129110.39252156877290423No Hit
TAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATAT127930.38893412046408204No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCCTGTAGGCACCATCAA126500.3845866195474586No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC120910.3675918432370215No Hit
TGGACCTGTCGTGGCGCCAGCCGCGCAGCAGGGCTGTAGGCACCATCAAT109680.33345028009458705No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA105450.320590189970589No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCA102220.31077031027779617No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT101740.30931100927081767No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTCTGTAGGCACCATCA94710.28793832993944507No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGATCTGTAGGCACCATCAA93250.2834996227098855No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAAT88040.2676601263633063No Hit
CTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC87770.266839269546881No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA87330.26550157695715065No Hit
TATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT86360.2625525728388816No Hit
TTTGGACAATGGGGGAAACCCTGATCCAGCTGTAGGCACCATCAATATCT86100.2617621181267683No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG85200.2590259287386836No Hit
TAGTCGAACAGAGACGAAAGTCGGTCATAGTGCTGTAGGCACCATCAATA82540.2509389689916777No Hit
AGTAGGAGGGTCTTTAAGGCCTGACTGCGCTGTAGGCACCATCAATATCT78830.2396597882919064No Hit
TAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT78260.23792686834611942No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC77560.2357987210442758No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGATATTGCTGTAGGCACCA77080.2343394200372973No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATAT73930.22476275717900088No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAA73850.2245195403445045No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG72430.2202024415321931No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCTGTAGGCACCAT70830.21533810484226476No Hit
AAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT70110.21314915333179701No Hit
AGCTGTCCGGGTACATGGTATCTGGGTGGCGGCTGTAGGCACCATCAATA69620.21165945022050647No Hit
TCAGAACGAACGCTGGCGGCATGCTTACTGTAGGCACCATCAATATCTCG68530.20834562085049277No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC67170.20421093466405368No Hit
AGTACCGGGAGAATAAGCTCCGGCTAACTCCGTGCTGTAGGCACCATCAA65240.19834332853182765No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCTGTAGGCACCAT63900.19426944655401268No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCATCTGTAGGCACCA62860.19110762770555928No Hit
TTTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATAT62060.1886754593605951No Hit
TTTGGACAATGGGGGAAACCCTGATCCAGCCTGTAGGCACCATCAATATC61910.1882194277959143No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC61790.1878546025441697No Hit
GATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT60880.18508801105177297No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA60700.18454077317415601No Hit
TCAGCTCGTGTCGTGAGATGTTGGGTTCTGTAGGCACCATCAATATCTCG59250.18013246804890848No Hit
TAGTCGAACATATCGTTAGATAGTTTGACCTGTAGGCACCATCAATATCT58780.17870356914624203No Hit
AGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTTCTGTAGGCACCATCAA58400.17754828918238405No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT58170.17684904078320685No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTTCTGTAGGCACCATC58060.17651461763577428No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAA56430.17155907463290979No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGATCTGTAGGCACCATCAA55050.1673635842378466No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT54190.16474900326701014No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA52450.15945903711671308No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA51310.15599319722513916No Hit
TCGACTTACTGGTCTATAGTTGACGCTGAGGTACGCTGTAGGCACCATCA50810.15447309200953654No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA50540.1536522351931111No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAA50440.1533482141499906No Hit
TTTCCGTTAGTAGTGGCGAGCGAACGCGGACTGTAGGCACCATCAATATC50300.15292258468962186No Hit
TATGGTTGGCTCAGGCCGGTTGGAAACCGGCTGCCTGTAGGCACCATCAA49720.15115926263952287No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA49700.15109845843089875No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGACTGTAGGCACCATCAATATCTCG49690.1510680563265867No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT49600.15079443738777823No Hit
GGGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCAT49210.14960875531960818No Hit
AAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT49120.14933513638079973No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCCTGTAGGCACCA48750.1482102585212538No Hit
TAACGATGATAACTAGCTGTCCGGGTACACTGTAGGCACCATCAATATCT48440.1472677932875802No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATCAATATC48030.14602130701078606No Hit
TTCAGCTCGTGTCGTGAGATGTTGGGTTCTGTAGGCACCATCAATATCTC44790.1361710252136812No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT43680.1327963916350434No Hit
ACCTACAGCTAAGGTCCCCAAGTCATGTCCTGTAGGCACCATCAATATCT43340.13176272008843362No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC43300.13164111167118545No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATATCT42630.12960417068227795No Hit
ATTCCGTTAGTAGTGGCGAGCGAACGCGGACTGTAGGCACCATCAATATC41530.12625993920795223No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC41360.12574310343464734No Hit
TGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATC41140.12507425713978218No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGCTGTAGGCACCATCAAT40860.12422299821904473No Hit
GTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC40720.123797368758676No Hit
TTAAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCT40350.12267249089913008No Hit
TTAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCA39960.12148680883096005No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGCTGTAGGCACCATCAATATCTCG39480.12002750782398154No Hit
CTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC39420.11984509519810922No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATC38510.11707850370571249No Hit
TGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAATA38490.11701769949708839No Hit
TGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCG38480.11698729739277633No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAATA38390.11671367845396786No Hit
TGGTTGGCTCAGGCCGGTTGGAAACCGGCTGCACTGTAGGCACCATCAAT37970.11543679007286169No Hit
TCGTCAGCTCGTGTCGTGAGATGTTGGGTTCTGTAGGCACCATCAATATC37630.1144031185262519No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT37450.11385588064863497No Hit
ACTGCTTGGTGGGGCTTGCTCTGCTAGGCGGCTGTAGGCACCATCAATAT37290.11336944697964213No Hit
TCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT37060.11267019858046494No Hit
AACGCTGGCGGCATGCTTAACACATGCCTGTAGGCACCATCAATATCTCG36790.11184934176403954No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCA36320.1104204428613731No Hit
GGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTCG36240.11017722602687668No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATAT36120.10981240077513205No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA35800.10883953343714639Illumina PCR Primer Index 6 (96% over 29bp)
AGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATATC35660.10841390397677765No Hit
TTTAAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATC35170.10692420086548711No Hit
TGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCT34870.10601213773612553No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGATATTGCTGTAGGCACCA34490.10485685777226757No Hit
ACCCACCCGATCCCATTTCGAACTCGGACGTGCTGTAGGCACCATCAATA33770.10266790626179982No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGCTGTAGGCACCATCAATAT33320.10129981156775747No Hit
TGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC33140.10075257369014054No Hit
CTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC32980.1002661400211477No Hit
AGCAATCTTTGACCCGTAGATTTCCGCTGTAGGCACCATCAATATCTCGT32950.10017493370821154No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)