FastQCFastQC Report
Tue 18 Jul 2023
SRR10401036_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10401036_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3220152
Total Bases164.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC1687885.241615923720371No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC944122.931911288659666No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT781982.4283946844745214No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG681742.117105031066856No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT669812.0800570904727476No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA645042.003135255727059No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA547111.6990191767345144No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC507341.5755156899425866No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAAT464461.4423542739597386No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT413951.2854983243027036No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA355671.1045130788857171No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC354461.1007554922873206No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT279310.8673814155356641No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAAT268960.8352400756237595No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT266530.8276938479922687No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA257240.7988442781582981No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT245590.7626658617357193No Hit
ATGGGGAAACCCATCCTCACCATTTAATTCTGTAGGCACCATCAATATCT232250.7212392458492642No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT229890.713910399260656No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA214960.6675461282572996No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCACTGTAGGCACCAT203660.6324546170491331No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG202860.6299702622733337No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATAT187250.5814942897105478No Hit
TTGAGTGAAGAAGGCCTTAGGGTTGTAAAGCTCCTGTAGGCACCATCAAT186560.5793515337164209No Hit
ACGATGATAACTAGCTGTCCGGGTACACTGTAGGCACCATCAATATCTCG184910.5742275519913346No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC174320.54134090564669No Hit
ATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCTC163430.5075226262611205No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA162070.5032992231422616No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC143630.4460348455600853No Hit
GTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT141650.4398860674899818No Hit
AGTACCGGGAGAATAAGCTCCGGCTAACTCCGCTGTAGGCACCATCAATA134150.41659524146686244No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACCA132870.4126202738255834No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT130100.404018195414378No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA125940.39109955058022106No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA124870.38777672606758934No Hit
TAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATAT124470.38653454867968967No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCCTGTAGGCACCATCAA123930.3848576092060251No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC115130.3575297066722316No Hit
TGGACCTGTCGTGGCGCCAGCCGCGCAGCAGGGCTGTAGGCACCATCAAT104200.3235872095478723No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA102990.3198296229494757No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCA99540.30911584297884076No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT97340.3022838673453924No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTCTGTAGGCACCATCA93380.28998631120518537No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGATCTGTAGGCACCATCAA88880.2760118155913137No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAAT87360.27129154151729484No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA86980.2701114729987901No Hit
CTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC86060.2672544650066208No Hit
TTTGGACAATGGGGGAAACCCTGATCCAGCTGTAGGCACCATCAATATCT84780.2632794973653418No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG83890.26051565267726495No Hit
TATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT81500.25309364278456425No Hit
TAGTCGAACAGAGACGAAAGTCGGTCATAGTGCTGTAGGCACCATCAATA79020.2453921429795861No Hit
AGTAGGAGGGTCTTTAAGGCCTGACTGCGCTGTAGGCACCATCAATATCT77540.24079608664435717No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGATATTGCTGTAGGCACCA76220.23669690126428816No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC76050.23616897587443075No Hit
TAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT74590.23163502840859687No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAA71330.22151128269721432No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCTGTAGGCACCAT70020.21744315175184276No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATAT69870.21697733523138038No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG69760.21663573644970796No Hit
AGCTGTCCGGGTACATGGTATCTGGGTGGCGGCTGTAGGCACCATCAATA67760.21042484951020948No Hit
AAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT66900.2077541681262251No Hit
TCAGAACGAACGCTGGCGGCATGCTTACTGTAGGCACCATCAATATCTCG65630.20381025491964352No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC65230.20256807753174386No Hit
AGTACCGGGAGAATAAGCTCCGGCTAACTCCGTGCTGTAGGCACCATCAA62820.19508395876964812No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCTGTAGGCACCAT62530.19418338016342085No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCATCTGTAGGCACCA61040.18955626939349446No Hit
TTTGGACAATGGGGGAAACCCTGATCCAGCCTGTAGGCACCATCAATATC60560.18806565652801482No Hit
TTTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATAT59900.18601606383798033No Hit
GATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT58820.18266218489065114No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC58420.18142000750275145No Hit
TCAGCTCGTGTCGTGAGATGTTGGGTTCTGTAGGCACCATCAATATCTCG57970.18002255794136426No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA57910.1798362313331793No Hit
AGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTTCTGTAGGCACCATCAA57840.17961885029029687No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT57760.1793704148127169No Hit
TAGTCGAACATATCGTTAGATAGTTTGACCTGTAGGCACCATCAATATCT57500.1785629995105821No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTTCTGTAGGCACCATC56550.1756128282143203No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAA54850.17033357431574658No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT53830.16716602197660235No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGATCTGTAGGCACCATCAA52800.16396741520276062No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA52160.16197993138212108No Hit
TCGACTTACTGGTCTATAGTTGACGCTGAGGTACGCTGTAGGCACCATCA50780.1576944193938671No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAA50690.1574149294815897No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT49550.15387472392607554No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA49370.15331574410152068No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA49340.1532225807974282No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGACTGTAGGCACCATCAATATCTCG48820.15160775019315859No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA48500.15061400828283883No Hit
TTTCCGTTAGTAGTGGCGAGCGAACGCGGACTGTAGGCACCATCAATATC48470.15052084497874635No Hit
GGGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCAT48190.14965132080721655No Hit
TAACGATGATAACTAGCTGTCCGGGTACACTGTAGGCACCATCAATATCT47750.14828492568052687No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATCAATATC47220.14663904064155978No Hit
AAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT47040.14608006081700492No Hit
TATGGTTGGCTCAGGCCGGTTGGAAACCGGCTGCCTGTAGGCACCATCAA46200.14347148830241555No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCCTGTAGGCACCA45950.14269512743497823No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATATCT43900.13632896832199226No Hit
TTCAGCTCGTGTCGTGAGATGTTGGGTTCTGTAGGCACCATCAATATCTC43380.13471413771772264No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC43170.1340619945890753No Hit
ACCTACAGCTAAGGTCCCCAAGTCATGTCCTGTAGGCACCATCAATATCT42170.13095655111932605No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT41660.12937277494975394No Hit
TGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATC41240.12806848869245924No Hit
ATTCCGTTAGTAGTGGCGAGCGAACGCGGACTGTAGGCACCATCAATATC40720.12645365808818962No Hit
GTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC40300.12514937183089495No Hit
TTAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCA39460.12254079931630557No Hit
TTAAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCT39210.12176443844886825No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGCTGTAGGCACCATCAAT38780.12042909775687607No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC38590.11983906349762372No Hit
TGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAATA38260.11881426715260647No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGCTGTAGGCACCATCAATATCTCG37620.11682678333196693No Hit
CTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC37490.11642307568089953No Hit
TGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCG37380.11608147689922711No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATC37000.11490140838072241No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT36920.11465297290314246No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAATA36540.11347290438463774No Hit
AACGCTGGCGGCATGCTTAACACATGCCTGTAGGCACCATCAATATCTCG36410.11306919673357034No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATAT36200.112417053604923No Hit
TGGTTGGCTCAGGCCGGTTGGAAACCGGCTGCACTGTAGGCACCATCAAT36190.11238599917022551No Hit
ACTGCTTGGTGGGGCTTGCTCTGCTAGGCGGCTGTAGGCACCATCAATAT36130.11219967256204054No Hit
TCGTCAGCTCGTGTCGTGAGATGTTGGGTTCTGTAGGCACCATCAATATC35390.10990164439442611No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCA34600.10744834405332418No Hit
TGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCT34530.10723096301044174No Hit
TCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT34390.10679620092467684No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGATATTGCTGTAGGCACCA34260.10639249327360945No Hit
ACCCACCCGATCCCATTTCGAACTCGGACGTGCTGTAGGCACCATCAATA34180.10614405779602949No Hit
GGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTCG34040.10570929571026459No Hit
TTTAAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATC33860.10515031588570975No Hit
AGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATATC33720.10471555379994485No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA32970.1023864711976329Illumina PCR Primer Index 6 (96% over 29bp)
TTCACGCTTTTGAGAACTCCACTGTCAACTGTAGGCACCATCAATATCTC32970.1023864711976329No Hit
AGCAATCTTTGACCCGTAGATTTCCGCTGTAGGCACCATCAATATCTCGT32830.101951709111868No Hit
TGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC32710.1015790558954981No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)