FastQCFastQC Report
Tue 18 Jul 2023
SRR10401037_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10401037_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3111171
Total Bases158.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT2263217.274463538005464No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT1920036.171406200430642No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA1596455.131347650129164No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC864622.779082216953038No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC832462.6757127782433043No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA683042.1954434519992634No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT554291.7816121325378773No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC446681.4357295050641703No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA436011.401433736686283No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC415331.3349635876652233No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA344371.1068822639449905No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA335671.0789185165328425No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC297370.9558137434425816No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT297290.9555566055353435No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT295630.9502209939601519No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT277860.8931042363148796No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC275400.8851972456673067No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA261410.8402302541390363No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA248300.7980917795903857No Hit
AGTACCGGGAGAATAAGCTCCGGCTAACTCCGCTGTAGGCACCATCAATA220990.7103113265069647No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA217050.6976472845754862No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC186670.5999991643018014No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCA173790.5585999612364605No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG169030.543300255755791No Hit
TGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC159240.5118330043575233No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA140660.4521127254014646No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA139570.44860922141534487No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC137480.4418914935887484No Hit
TTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA133430.4288738870348174No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC129860.4173991079243153No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC122160.3926495843526441No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATAT120180.3862854211485No Hit
TCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT117080.376321327243022No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT116610.3748106420379979No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG100180.3220009443389643No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA94960.3052226958916755Illumina PCR Primer Index 6 (96% over 29bp)
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC94490.30371201068665143No Hit
ACTGCTTGGTGGGGCTTGCTCTGCTAGGCGGCTGTAGGCACCATCAATAT91460.2939729124500068No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA91100.2928157918674351No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT89660.28818730953714855No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATCAATATC81460.2618306740452389No Hit
GTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT81180.2609306913699054No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCAATATCTC80650.25922715273445274No Hit
TGGACCTGTCGTGGCGCCAGCCGCGCAGCAGGGCTGTAGGCACCATCAAT79920.25688076933090465No Hit
CTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC79290.2548558083114043No Hit
TAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT74850.24058465445968738No Hit
AGTACCGGGAGAATAAGCTCCGGCTAACTCCGTGCTGTAGGCACCATCAA73620.23663115913590094No Hit
ACGGGGCTCAAGACATGCACCGAAGCTTAGGATCTGTAGGCACCATCAAT70230.22573494031668465No Hit
AAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT70190.22560637136306555No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCAA69380.22300285005227935No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT68710.22084932007915992No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT65590.21082094169687232No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGCTGTAGGCACCATCAAT64270.206578166227443No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG63750.20490676983039505No Hit
GGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTCG63020.202560386426847No Hit
GTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCG62840.20198182613556118No Hit
ATGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC62720.20159611927470397No Hit
GTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAAT62460.20076042107618No Hit
TTTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATAT62250.20008543406967988No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTC60980.19600336979227434No Hit
CCGCCCGTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAA59380.1908606116475115No Hit
CGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGTA59250.19044276254824952No Hit
GGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTC55790.17932154806019984No Hit
TCAGCTCGTGTCGTGAGATGTTGGGTTCTGTAGGCACCATCAATATCTCG55290.17771443613996143No Hit
AGGTAGCCGTAGGGGAACCTGCGGCTGGATCCTGTAGGCACCATCAATAT55060.1769751646566518No Hit
TTGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT55000.17678231122622318No Hit
GGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT53950.17340737619372257No Hit
TAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATC51470.16543610106934012No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG51430.16530753211572105No Hit
TGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTC50980.16386113138750652No Hit
TTCAGCTCGTGTCGTGAGATGTTGGGTTCTGTAGGCACCATCAATATCTC48830.15695055013048143No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCCTGTAGGCACCA48530.1559862829783384No Hit
CCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT45800.14721145189383675No Hit
GGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA44810.14402937029176474No Hit
TTGAGGTAATCACGCCGGAGGATTATCTCGCTGTAGGCACCATCAATATC44180.14200440927226438No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA42160.13551167711450127No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT41050.13194388865157203No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTCGTAT40090.12885823376471434No Hit
CGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT39260.1261904279771186No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT39020.12541901425540417No Hit
TGTTGTTTTCTGATGTCTAACCTAGAACCACTGTAGGCACCATCAATATC38150.12262263951418936No Hit
GAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC37650.12101552759395096No Hit
CTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT37120.11931198895849826No Hit
CTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC36730.1180584416607123No Hit
CGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA36270.116579898694093No Hit
TAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGCTGTAGGCACCATCAA34420.11063358458921094No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAA34350.11040858892037755No Hit
TGTTGTTTTCTGATGTCTAACCTAGAACCATGCTGTAGGCACCATCAATA33780.1085764813313058No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATC33610.10803006327842475No Hit
TTTTTCTGATGTCTAACCTAGAACCATGCTGTAGGCACCATCAATATCTC33510.10770864089437707No Hit
ACCGGGAGAATAAGCTCCGGCTAACTCCGTGCTGTAGGCACCATCAATAT33100.10639080911978159No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC32620.10484798167635272No Hit
TTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAAT32020.10291944737206667No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)