FastQCFastQC Report
Tue 18 Jul 2023
SRR10401044_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10401044_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4151393
Total Bases211.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT2246205.4107139458971965No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT2119455.105394743402997No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA1777264.281117205718659No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC1777114.280755881218665No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC1392033.3531636248362897No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA1178552.8389265964460604No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA775911.8690352852644883No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT570451.3741170734738917No Hit
AGTACCGGGAGAATAAGCTCCGGCTAACTCCGCTGTAGGCACCATCAATA564441.3596400051741668No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC513821.2377050305764836No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA507511.2225053132767723No Hit
TTGAGTGAAGAAGGCCTTAGGGTTGTAAAGCTCCTGTAGGCACCATCAAT413470.9959789400810763No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA335400.8079215819846495No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA319910.7706088052853585No Hit
TGGACCTGTCGTGGCGCCAGCCGCGCAGCAGGGCTGTAGGCACCATCAAT314900.7585405669855877No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA271100.6530338129875923No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG240480.5792754383889938No Hit
AGTACCGGGAGAATAAGCTCCGGCTAACTCCGTGCTGTAGGCACCATCAA237180.5713262993891448No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT233970.5635939550892917No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA225990.5443714916896569No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA214240.5160677391901948No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC213450.5141647634902309No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA201180.4846084193907924No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC192030.46256762489121117No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT191330.46088144389124325No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCA189560.4566178147913243No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC189340.45608787219133434No Hit
TCGACTTACTGGTCTATAGTTGACGCTGAGGTACGCTGTAGGCACCATCA189090.4554856646913458No Hit
ACCGCCCGTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCA181370.4368894970916991No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA174990.4215211616919911No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT169810.4090434222922282No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG166490.40104610669238017No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC165350.3983000404924323No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT163930.3948795018924973No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC158020.3806433165927678No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATAT155420.37438035859288676No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAAT142510.34328236329347767No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATATCT142290.3427524206934877No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATAT140670.33885011609356186No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATCAATATC131730.317315175893971No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC125450.30218772349425843No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCA123910.29847812529432893No Hit
TCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT123130.2965992378943646No Hit
CCGCCCGTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAA117690.2834952026946136No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT115340.27783445219472114No Hit
ATTCCGTTAGTAGTGGCGAGCGAACGCGGACTGTAGGCACCATCAATATC115040.2771118031947349No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAA110250.2655735074949541No Hit
TCAGCTCGTGTCGTGAGATGTTGGGTTCTGTAGGCACCATCAATATCTCG110080.2651640063949619No Hit
AAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT109600.2640077679949838No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA107640.25928646119507354No Hit
ACCGGGAGAATAAGCTCCGGCTAACTCCGTGCTGTAGGCACCATCAATAT105400.25389068199517606No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC103860.25018108379524656No Hit
TTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATATCTCG103330.2489044038952708No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC101040.2433881831953756No Hit
ACGATGATAACTAGCTGTCCGGGTACACTGTAGGCACCATCAATATCTCG94520.22768261159567404No Hit
TTCAGCTCGTGTCGTGAGATGTTGGGTTCTGTAGGCACCATCAATATCTC94500.22763443499567493No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGCTGTAGGCACCATCAAT94410.22741764029567904No Hit
TGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC93320.22479201559572898No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCGCTGTAGGCACCATCAAT91000.21920352999583512No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA90050.21691514149587862No Hit
TAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT89470.21551802009590515No Hit
TTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA88600.21342233799594498No Hit
TTTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATAT87190.2100258876960095No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAAT85460.2058586117960887No Hit
TTTCCGTTAGTAGTGGCGAGCGAACGCGGACTGTAGGCACCATCAATATC84810.20429287229611842No Hit
TGGATGAAGGTAAGGTAACACTTACTGGAGGTCCGCTGTAGGCACCATCA84350.20318481049613948No Hit
TGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC80590.19412760969631157No Hit
TTTCCGTTAGTAGTGGCGAGCGAACGCGGAGCTGTAGGCACCATCAATAT79920.19251369359634224No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATC77720.1872142675964429No Hit
AGCTGTCCGGGTACATGGTATCTGGGTGGCGGCTGTAGGCACCATCAATA77220.1860098525964658No Hit
GAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC77160.18586532279646856No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT74440.17931330519659305No Hit
TTCGCCAATTAAAGTGGTACGTGAGCTGGGTTCTGTAGGCACCATCAATA73120.17613364959665345No Hit
TTGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC71040.17112328319674866No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT70330.16941301389678115No Hit
ACTGCTTGGTGGGGCTTGCTCTGCTAGGCGGCTGTAGGCACCATCAATAT64730.15592356589703746No Hit
TTGGACCTGTCGTGGCGCCAGCCGCGCAGCAGGGCTGTAGGCACCATCAA61160.14732404279720085No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATC60160.14491521279724662No Hit
GGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA58840.14173555719730702No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAA57260.13792960579737934No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT57090.13752010469738712No Hit
AAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC55970.13482221509743839No Hit
CTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC55500.1336900649974599No Hit
GTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAAT54010.13010090829752807No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCTGTAGGCACCATCAA52470.12639131009759855No Hit
TAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGCTGTAGGCACCATCAA51280.12352480239765304No Hit
AATACGGAGGGAGCTAGCGTTGTTCGGCTGTAGGCACCATCAATATCTCG50710.12215176929767911No Hit
GTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCG50020.1204896765977107No Hit
AGGCCCTTATTATAGGCAACTATTCTAAGGGGCTGTAGGCACCATCAATA49100.11827355299775281No Hit
AGGTTGGGGTCGAGGGTTCGAATCCCTTTGCCCGCCTGTAGGCACCATCA48550.11694869649777798No Hit
CCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT47990.11559975169780361No Hit
GGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTC47300.11393765899783519No Hit
GTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT46820.11278142059785715No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAAT46690.11246827269786311No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCCTGTAGGCACCATCA46410.11179380029787592No Hit
GGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA45970.11073391509789605No Hit
TACAGTACCGGGAGAATAAGCTCCGGCTAACTCCGCTGTAGGCACCATCA45890.11054120869789973No Hit
ATGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC45750.11020397249790613No Hit
TCAGAACGAACGCTGGCGGCATGCTTACTGTAGGCACCATCAATATCTCG45050.10851779149793817No Hit
GGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT44670.10760243609795556No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT44570.10736155309796012No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA43860.10565128379799263No Hit
TGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCT42590.10259206969805074No Hit
ATTCCGTTAGTAGTGGCGAGCGAACGCGGAGCTGTAGGCACCATCAATAT42380.10208621539806037No Hit
TAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATC42210.10167671429806814No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)