FastQCFastQC Report
Tue 18 Jul 2023
SRR10401045_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR10401045_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5646354
Total Bases287.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC3347865.929242126866293No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC2168203.8400001133474806No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC2132523.7768088929599526No Hit
TCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGTA1863333.30005876358443No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA1453722.57461717773983No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA1410052.4972752328316647No Hit
TTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT1251572.2165985342045507No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAAT1096091.9412349987266118No Hit
TTTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCT1023231.8121959763769684No Hit
TGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCG936271.6581850872261994No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA737131.305497317383926No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCA630151.1160299194843257No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT620151.0983193756537404No Hit
ATGGGGAAACCCATCCTCACCATTTAATTCTGTAGGCACCATCAATATCT579911.0270521472794658No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC558620.9893463994641497No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAAT549770.9736725681740819No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCT420720.74511800004038No Hit
TTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC378800.6708754003025669No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCACTGTAGGCACCAT373060.660709548143811No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGATATTGCTGTAGGCACCA328410.5816319699402481No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC317730.5627171091291832No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA305260.5406320609724434No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGACTGTAGGCACCATCAAT290110.5138005870691069No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATAT277630.4916978283685366No Hit
AAGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCG263070.4659112765512045No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCGCTGTAGGCACCAT261910.4638568534668566No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCATC261320.4628119313808522No Hit
GTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT254370.4505031034185954No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA253510.44897999664916505No Hit
AGGTCGTGAGTTCAATCCTCACCGGCAGCACCACTGTAGGCACCATCAAT253260.44853723305340054No Hit
TTGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA243290.4308798208543071No Hit
TAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCAT224530.3976548406281292No Hit
ACGATGATAACTAGCTGTCCGGGTACACTGTAGGCACCATCAATATCTCG222050.39326262575814414No Hit
TGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATAT207250.36705102088887803No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCCTGTAGGCACCA201310.3565309578535104No Hit
TTGAGTGAAGAAGGCCTTAGGGTTGTAAAGCTCCTGTAGGCACCATCAAT197940.35056250458260324No Hit
AGTGGAGGAGTGCAAGCTCTGAAATGCTGTAGGCACCATCAATATCTCGT197370.34955300358425984No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCA175080.31007620138588543No Hit
TGGATGAAGGTAAGGTAACACTTACTGGAGGTCCGCTGTAGGCACCATCA170630.3021950093812751No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCCTGTAGGCACCA169240.2997332437888237No Hit
ATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCTC164160.29073628752288644No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC163800.2900987079449854No Hit
TGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAA155640.2756469041792278No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGATATTGCTGTAGGCACCA155590.27555835146007496No Hit
AGCGGTTCGGGAAGCACCGGGCAACAGAAGCTTCCCATCTGTAGGCACCA154730.2740352446906446No Hit
TAGTCGAACATATCGTTAGATAGTTTGACTGCTGTAGGCACCATCAATAT146870.26011475723980465No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCCTGTAGGCACCATCAA146340.25917609841678363No Hit
TTTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCG144820.2564840957545347No Hit
GAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC143110.25345559275950463No Hit
AAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT141870.2512594853245121No Hit
TAGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGACCCTGTAGGCACCAT137540.24359081984586867No Hit
TGGACCTGTCGTGGCGCCAGCCGCGCAGCAGGGCTGTAGGCACCATCAAT136310.2414124229547067No Hit
TAACCGTCGTAGGGGTACAACCCCTACCGAGGGTTCCTGTAGGCACCATC134180.23764007711879206No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATA133700.23678997101492394No Hit
TTGGGGAAACCCATCCTCACCATTTAATTTCGCTGTAGGCACCATCAATA133130.23578047001658062No Hit
TTTGGACAATGGGGGAAACCCTGATCCAGCTGTAGGCACCATCAATATCT131090.23216751907514124No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA129540.22942238478140053No Hit
TTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCA128050.22678351375064335No Hit
TTTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATAT127760.22626990797955637No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATATCT125750.22271008866960873No Hit
AGCTGTCCGGGTACATGGTATCTGGGTGGCGGCTGTAGGCACCATCAATA116690.20666433595909856No Hit
CTGTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTC113310.20067817214436076No Hit
ATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAAT112480.19920819700642217No Hit
GGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTCG112320.19892482830513283No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATAT110890.19639222053735916No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCA108650.19242505871930807No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCTGTAGGCACCATCAATATCTCGT105000.18596071022114447No Hit
TAACGATGATAACTAGCTGTCCGGGTCTGTAGGCACCATCAATATCTCGT104660.1853585517309046No Hit
TAGTCGAACAGAGACGAAAGTCGGTCATAGTGCTGTAGGCACCATCAATA102320.18121428447454763No Hit
AGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACTGTAGGCACCATC101710.18013394130088195No Hit
GGTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATC101550.17985057259959258No Hit
AGTACCGGGAGAATAAGCTCCGGCTAACTCCGCTGTAGGCACCATCAATA99480.17618449002666145No Hit
TTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATATCTCG99100.17551148936109923No Hit
AGGATGAAGGTAAGGTAACACTTACTGGAGGTCCGCTGTAGGCACCATCA97690.1730143026809867No Hit
ACCGCCCGTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCA96090.1701806156680931No Hit
TTGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCG94970.16819703475906753No Hit
TGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAA92910.164548662729967No Hit
ATGGGGAAACCCATCCTCACCATTTAATTTCGATCTGTAGGCACCATCAA92110.16313181922352016No Hit
GTGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGACCTGTAGGCACCAT88750.15718107649644353No Hit
AGTAGGAGGGTCTTTAAGGCCTGACTGCGCTGTAGGCACCATCAATATCT88500.1567383129006789No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAAT87110.15427654730822757No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGCTGTAGGCACCATCAATATCTCGT86850.15381607316863236No Hit
TCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTCGT86730.15360354664266535No Hit
CTGGGTTCAGAACGTCGCGAGACAGTTTGGTCCCTCTGTAGGCACCATCA86420.1530545197839172No Hit
CTCTGACTGGCTTAGGTTAGTTAGAGCTGTAGGCACCATCAATATCTCGT86030.15236380857452436No Hit
TCAGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTCTGTAGGCACCATCA84820.15022083277102355No Hit
CTGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC83210.14736943521429935No Hit
TTTGGACAATGGGGGAAACCCTGATCCAGCCTGTAGGCACCATCAATATC82990.1469798032500265No Hit
TTTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGCTGTAGGCACCATC82820.14667872400490653No Hit
GGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATATCTC82680.14643077639127833No Hit
TTGTCTGACTGGCTTAGGTTAGTTAGACTGTAGGCACCATCAATATCTCG81910.14506706451632329No Hit
ACTGCCTTTGAGACTGTTAGACTAGCTGTAGGCACCATCAATATCTCGTA81240.14388045807967406No Hit
TATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT80450.14248132511705783No Hit
AGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTCTGTAGGCACCATCAAT79930.1415603768378674No Hit
GGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCTC79910.14152495575020624No Hit
CTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATCAATA77800.13778803100195275No Hit
AGGCGCTGCGCTAACCCGCAAGGGAGGCAGGCGCTGTAGGCACCATCAAT76890.1361763715133695No Hit
TATGGATGCGGGGGTTTCGCGACCTACCAAATCTCTGTAGGCACCATCAA74800.1324748678527772No Hit
TTTCCGTTAGTAGTGGCGAGCGAACGCGGACTGTAGGCACCATCAATATC74680.13226234132681017No Hit
AAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACC73910.13089862945185513No Hit
TCGACGGACCCGGAGGGCAGTACTCCGGCGCCTCCACCTGTAGGCACCAT72890.12909215398113544No Hit
TGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATCT71740.12705544144061814No Hit
GATGCGGGGGTTTCGCGACCTACCAAATCCTGTAGGCACCATCAATATCT70950.1256563084780019No Hit
TGTATCGGGTCGTTCCGGCTTCCACCCTGTAGGCACCATCAATATCTCGT70270.12445199149752212No Hit
CTCACACCATGGGAGTTGGATTCACCCGCTGTAGGCACCATCAATATCTC69170.12250383167615773No Hit
TCGACTTACTGGTCTATAGTTGACGCTGAGGTACGCTGTAGGCACCATCA68440.12121096197652502No Hit
GCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATC67140.11890859127854894No Hit
TTGGGAAACAGGTTAATATTCCTGTACCTGGCTGTAGGCACCATCAATAT65960.11681874710653989No Hit
TGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCAT65570.11612803589714708No Hit
TGGTCGTGAGTTCAATCCTCACCGGCAGCACCCTGTAGGCACCATCAATA65560.11611032535331649No Hit
AGCCCTCGCTTCACGCCCCCGAAGCGAGGGCCTTCTGTAGGCACCATCAA65510.11602177263416358No Hit
TGTGGGGGTCGCTGGTTCGAATCCAGTCGCCCCGCTGTAGGCACCATCAA65080.1152602192494484No Hit
TCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCATCAATATCTC63780.11295784855147233No Hit
TTGGGGAAACCCATCCTCACCATTTAATTCTGTAGGCACCATCAATATCT63580.11260363767486062No Hit
TCTACGGCGAGATGTGAGCAATCTTTGACCCGTAGCTGTAGGCACCATCA62820.11125763634373614No Hit
CAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCCTGTAGGCACCATC60570.10727276398185448No Hit
GAGGCTGAGGTCGTGAGTTCAATCCTCACCGGCAGCACCTGTAGGCACCA60090.10642265787798641No Hit
ATGGGAAACAGGTTAATATTCCTGTACCTGCTGTAGGCACCATCAATATC59370.10514749872218426No Hit
ATTCCGTTAGTAGTGGCGAGCGAACGCGGACTGTAGGCACCATCAATATC59130.10472244567025023No Hit
TAACGATGATAACTAGCTGTCCGGGTACACTGTAGGCACCATCAATATCT58310.10327018107614225No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)