FastQCFastQC Report
Thu 29 Jun 2023
SRR1040415.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1040415.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140321514
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAA84985956.056516037875703No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAA64565714.601269481741766No Hit
TTGCTCCACACAATATAGATGGATACGAATAAGGCG34204342.437569195554717No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCG24621811.7546710620582389No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAA20893521.4889748125152071No Hit
TGCTCCACACAATATAGATGGATACGAATAAGGCGT15962281.1375504400558278No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAA15844621.1291654108007985No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAA14762251.0520304106753011No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAAA13189010.9399136044099411No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGA10744070.7656751765092842No Hit
AGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAA10726700.7644373050307881No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCA10638650.758162429746874No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCC10153250.7235704426621281No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGC8845630.630383021665516No Hit
GAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAA7315590.5213448594917527No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAA6844650.48778336299877717No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAA5916070.4216081933095448No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGAA5407260.3853478946927554No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCC5370590.3827346104603746No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAAA5168230.3683134433683491No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAA5040590.35921719031623334No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAAAA5030470.358495989431813No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAA4657690.3319298564580767No Hit
ACACAATATAGATGGATACGAATAAGGCGTCAAAAA4642850.3308722852006856No Hit
AATATAGATGGATACGAATAAGGCGTCAAAAAAAAA4531180.32291413275372727No Hit
AGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAA4292360.30589464706032177No Hit
TTTGCTCCACACAATATAGATGGATACGAATAAGGC3985670.2840384119572712No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAA3844550.2739815079247221No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAA3771440.26877133038915185No Hit
CAATATAGATGGATACGAATAAGGCGTCAAAAAAAA3697000.2634663705239098No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCAAA3354270.2390417480814809No Hit
ATATAGATGGATACGAATAAGGCGTCAAAAAAAAAA3316110.23632227913390388No Hit
TGCGGGAATACTGCCAGCTGGGACTGAGGACTGCGA3298720.23508298235721714No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATCTAAA3148500.22437756764796596No Hit
GTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTAA3112960.22184481276335144No Hit
GCGGGAATACTGCCAGCTGGGACTGAGGACTGCGAC3053320.21759457355911938No Hit
CGGGAATACTGCCAGCTGGGACTGAGGACTGCGACG2782290.19827964512982663No Hit
AGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAA2755910.1963996768164859No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATAA2435090.1735364685418089No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAA2431920.1733105587786061No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAA2416240.17219312499721176No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAA2394710.17065879149508037No Hit
TAGCCTGCGGGAATACTGCCAGCTGGGACTGAGGAC2310130.16463120544722742No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAA2275090.1621340830173768No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAA2256660.16082067073478126No Hit
AAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAA2217760.1580484657541537No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGG2108900.1502905677029682No Hit
CCACACAATATAGATGGATACGAATAAGGCGTCAAA2078690.14813765478613636No Hit
AGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAA2069740.14749983384586343No Hit
TAGCCTGCGGGAATACTGCCAGCTGGGACTGAGGAA2054960.146446538483044No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCGT2053570.1463474802588005No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAA2028680.14457369666065603No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAA2018210.14382755305790101No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCAAAAAA2009100.14317832973210365No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAA2003370.14276998180050993No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAA1985040.14146369600886716No Hit
AGCGAAATGCGATACGTAATGTGAATTGCAGAAAAA1955520.13935995587960945No Hit
GCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAA1950840.13902643610301982No Hit
AGTTAAAAAGCTCGTAGTTGAACTTTGGAAAAAAAA1880970.13404715687431937No Hit
AGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAA1875410.13365092397734532No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGAAAAAAA1844840.13147235569308352No Hit
ATGATTAATAGGGACGGTCGGGGGCAAAAAAAAAAA1825480.13009266704462724No Hit
AAGCCATGCATGTCTAAGTATAAGCAAAAAAAAAAA1762820.1256272078136215No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAA1715200.12223357282191241No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAA1705930.1215729471105906No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAA1696390.12089307987369635No Hit
GCTCCACACAATATAGATGGATACGAATAAGGCGTC1689420.12039636345428828No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTA1677860.11957254110014806No Hit
TATAGATGGATACGAATAAGGCGTCAAAAAAAAAAA1675950.11943642512295015No Hit
ATAGATGGATACGAATAAGGCGTCAAAAAAAAAAAA1665630.11870097125662427No Hit
TGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAA1657470.11811944959487823No Hit
AGATTAAGCCATGCATGTCTAAGTATAAGCAAAAAA1620260.11546768231135249No Hit
ATTAAGCCATGCATGTCTAAGTATAAGCAAAAAAAA1610010.11473721698869355No Hit
TAAGCCATGCATGTCTAAGTATAAGCAAAAAAAAAA1609850.11472581460316912No Hit
ATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAA1585530.11299265200345543No Hit
AAGCGTGTTACCTATACTCTACCGTCAGGGTTGAAA1579570.11256791314267034No Hit
CCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCAAAA1556150.11089888896153159No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGAAAAAAA1524170.10861983715483572No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAAAAAAAAA1502410.10706911272351295No Hit
TGTGAAACTCCGTCGTGCTGGGGATAGAGCATAAAA1442370.10279036755546979No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAAAA1433340.10214684542243464No Hit
AGCGGCAAAAGCTCAAATTTGAAATCTGGTAAAAAA1430710.1019594187103768No Hit
AAGATTAAGCCATGCATGTCTAAGTATAAGCAAAAA1408780.1003965792444343No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)