FastQCFastQC Report
Tue 27 Jun 2023
SRR1040423.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1040423.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130172032
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAA119917779.212253059090298No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAA51036663.9207085589629576No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAA31507132.4204223838189756No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAA30245242.3234822054556235No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAAAA29882612.2956244548752225No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAA29840502.2923895049898277No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAA27209982.090309230173191No Hit
AGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAA20262351.5565824462200912No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAA14217261.0921900642989117No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAAA13628611.0469691369648435No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACAAAAA12720960.9772421774901693No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAA11292490.8675050874215438No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAA9640390.7405884237867625No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAA9579350.7358992444705788No Hit
GAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAA9532320.7322863332117301No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAA9356100.718748863043023No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAA9091000.6983835052985883No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAAA8374200.6433179133287249No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAA7983930.6133368187722537No Hit
AGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAA7194670.5527047468998564No Hit
ATATAGATGGATACGAATAAGGCGTCAAAAAAAAAA6758180.5191729664326051No Hit
AAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAA6145070.47207298722969926No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCACTAAAA4697900.3608993366562796No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAA4287200.3293487805429664No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAA4198080.32250245582707043No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGAAA4115300.3161431788972918No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAA4060040.3118980273734991No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAA4037860.310194128336262No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCACTAAAA3979100.3056801018516788No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAA3835610.29465699667344825No Hit
AATATAGATGGATACGAATAAGGCGTCAAAAAAAAA3690210.28348716258804346No Hit
TATAGATGGATACGAATAAGGCGTCAAAAAAAAAAA3597610.27637349933970456No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACAAAA3543690.2722312885151858No Hit
AGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAA3367160.2586700037070943No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAA3289480.25270251600589594No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCG3242320.2490796179627894No Hit
AGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAA3241180.24899204154698912No Hit
TGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAA3201750.24596297306014245No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACCAAAA2738000.21033704075542123No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAA2653190.20382181634838428No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAA2615880.20095560926635916No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGAA2528710.1942590863143321No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2351040.1806102250904403No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATAA2250400.17287891764645727No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAA2201810.16914616497651352No Hit
AAGAGGTGCACAATCGACCGATCCCGAAAAAAAAAA2197200.16879201824244397No Hit
AAATATAGATGGATACGAATAAGGCGTCAAAAAAAA2150410.16519754412376383No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCACAAAAA2146580.16490331809524184No Hit
GGGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAA2136840.164155077490071No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAAAAAA2097860.1611605786410402No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAA2064340.15858552473084234No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAA2054710.15784573448158204No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAA2048260.1573502363395541No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCAAA1973780.1516285771739355No Hit
AGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAA1963050.15080428336556967No Hit
TAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAA1874290.1439856143599264No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATCAAAA1859390.1428409752411332No Hit
GCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAA1827520.14039267666959365No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCACAAAAA1826050.14027974918606173No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATCTAAA1822740.1400254702945714No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGATGAAAA1802010.13843296231251886No Hit
ATGATTAATAGGGACGGTCGGGGGCAAAAAAAAAAA1796630.13801966308707542No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACAAAA1751540.13455578537792204No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCA1748890.13435220862189506No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAA1712320.13154284938872277No Hit
CAATATAGATGGATACGAATAAGGCGTCAAAAAAAA1689730.1298074535703645No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATCAAAA1656580.12726082358459304No Hit
GAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAA1655340.1271655650270559No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAA1598830.1228243867315523No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAA1574270.12093765272097771No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAA1513900.11629994375443105No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAA1496290.11494711859456877No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAA1453250.11164072479102118No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACTAAAA1419430.1090426244556127No Hit
CCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCAAAA1405670.1079855617526198No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCC1403280.10780195856510869No Hit
ATAGATGGATACGAATAAGGCGTCAAAAAAAAAAAA1401960.10770055429418202No Hit
GGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAAA1397910.10738942755383891No Hit
CTTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAA1341970.10309203746623546No Hit
AGAGGTGCACAATCGACCGATCCGAAAAAAAAAAAA1335390.1025865525399496No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAA1308800.10054387105211664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)