FastQCFastQC Report
Tue 27 Jun 2023
SRR1040427.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1040427.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171400626
Total Bases6.1 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAA155307669.06109059368313No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAA66075573.855036678804195No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAA39440242.301055773273547No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAA39417472.2997273067135704No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAAAA37570392.1919634062480027No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAA36734582.1431998737274154No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAA34178401.994065062516166No Hit
AGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAA25640381.4959326928012504No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAAA19272491.124411879335843No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAA17484861.0201164609515487No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACAAAAA16156570.9426202445725023No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAA13691870.7988226367387946No Hit
GAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAA12372770.7218625910969543No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAA11966370.6981520592579399No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAA11727420.6842110366621414No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAA11699950.6826083587349324No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAA11510890.6715780606308871No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAAA10975180.640323215622328No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAA10088970.5886192037595008No Hit
ATATAGATGGATACGAATAAGGCGTCAAAAAAAAAA9160460.5344472895915794No Hit
AGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAA9037610.5272798711948695No Hit
AAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAA7911880.4616015813151114No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCACTAAAA5825860.33989724168218616No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAA5450700.3180093402926078No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAA5382120.3140081880447741No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAA5306970.3096237233112556No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGAAA5211640.3040618999839592No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAA5134510.29956191641913843No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAA5049030.29457477010614885No Hit
AATATAGATGGATACGAATAAGGCGTCAAAAAAAAA4962660.2895356986619174No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCACTAAAA4879550.284686824889426No Hit
TATAGATGGATACGAATAAGGCGTCAAAAAAAAAAA4837720.282246343721055No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACAAAA4640410.27073471715325004No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAA4530110.26429950144989556No Hit
TGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAA4420960.25793138001724686No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCG4412750.257452385267251No Hit
AGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAA4176920.2436933923450198No Hit
AGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAA3988600.23270626794560248No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACCAAAA3477810.20290532661181765No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAA3391830.19788900887678204No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAA3376580.1969992805043781No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3358770.19596019445109847No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGAA3283780.19158506457263466No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATAA3157720.18423036564638917No Hit
AAATATAGATGGATACGAATAAGGCGTCAAAAAAAA2853370.1664737210469698No Hit
AAGAGGTGCACAATCGACCGATCCCGAAAAAAAAAA2787750.16264526361764864No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAA2771670.16170711068464824No Hit
GGGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAA2749430.16040956583204077No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAAAAAA2747200.16027946128971549No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAA2664200.15543700523007425No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCACAAAAA2628990.1533827536895927No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAA2594690.15138159413723493No Hit
TAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAA2565840.14969840308517893No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAA2553970.14900587352580616No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCAAA2473460.14430869114795414No Hit
AGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAA2467940.14398663864856595No Hit
CAATATAGATGGATACGAATAAGGCGTCAAAAAAAA2372430.138414313609333No Hit
GCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAA2346890.13692423737122172No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATCTAAA2309190.13472471214895096No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGATGAAAA2296470.13398259117209993No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATCAAAA2291530.13369437752228513No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACAAAA2259270.1318122373718752No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCA2242010.13080523988284617No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCACAAAAA2212000.12905437113164336No Hit
ATGATTAATAGGGACGGTCGGGGGCAAAAAAAAAAA2192140.12789568224797498No Hit
GAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAA2135240.1245759744191366No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAA2093180.12212207439662444No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAA2045520.11934145444719671No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATCAAAA2012000.11738580231323076No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAA2009220.11722360920665482No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAA1936430.11297683358519356No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAA1924480.1122796365982934No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCC1899760.1108374014923376No Hit
ATAGATGGATACGAATAAGGCGTCAAAAAAAAAAAA1861140.10858420085350212No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAA1838930.10728840628621741No Hit
CCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCAAAA1815810.10593951973080892No Hit
GGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAAA1814410.10585783974908004No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACTAAAA1793810.10465597716078354No Hit
TAGCCTGCGGGAATACTGCCAGCTGGGACTGAGGAA1769030.10321024148418222No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGA1766320.10305213237669272No Hit
CTTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAA1745720.10185026978839622No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)