FastQCFastQC Report
Tue 27 Jun 2023
SRR1041221.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041221.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50000000
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG32560156.51203No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT2528240.505648No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC2392420.478484No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC2348890.46977800000000003No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT2235620.44712399999999997No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT2082590.416518No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA1911670.382334No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT1779230.355846No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT1759850.35197No Hit
TGCTGCGTTACTTACCACGAATTGCAGACGCTTAGAGTGGTGAAATTTCG1403870.280774No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA1395880.279176No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG1388380.27767600000000003No Hit
TACTCCGGAGTTAACAGAAAATCATACCTAATCTAGTACTTATAGTAAGG1147960.229592No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA1099870.219974No Hit
AAGAGAGGAGAAGGCTTAGTAGTTTAGACTGTTCTTCTATTAATTAATCT1050670.21013400000000002No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT1047640.20952800000000002No Hit
CAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGC949320.189864No Hit
GGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTA929920.185984No Hit
TAGTACCTGGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTA880840.17616800000000002No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA825090.165018No Hit
GGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTAAACTGAAA818140.163628No Hit
TACCACGAATTGCAGACGCTTAGAGTGGTGAAATTTCGAACGCATAGCAC808490.161698No Hit
AGTACCTGGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTAA793410.15868200000000002No Hit
CAGCCGGAATGTTAAGATCTTTAGTAATATTTTTTAAATTTGGACTATAC785300.15705999999999998No Hit
AGTACTTATAGTAAGGTAAGTTTTACATCGATGTTGTATTCAGATAATCT779750.15594999999999998No Hit
GACGTGATATTAGTTTAATTCATTGTGAGATAGAATTGTTTATCTTGATA748420.14968399999999998No Hit
ATGGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTT748070.149614No Hit
GGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAA747350.14947000000000002No Hit
ACTCCGGAGTTAACAGAAAATCATACCTAATCTAGTACTTATAGTAAGGT738950.14779No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG705440.141088No Hit
CTATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATT690070.138014No Hit
GGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTGTT596370.119274No Hit
AGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTT595200.11904No Hit
TGTACTTTAATTGATGTTAATTATGAAATTATTATATTTTCTTTTAGATC592360.118472No Hit
TCCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGA585500.1171No Hit
TTCAGGGAATTAAAATTTGATCATGGTTTAAGATGATTTAAAATGGTATT569410.113882No Hit
AAATGGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCT551220.11024400000000001No Hit
ACCTCGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTA502880.10057600000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)