FastQCFastQC Report
Tue 27 Jun 2023
SRR1041222.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041222.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6772541
Total Bases345.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG4344126.414313327892736No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT330550.4880738263526201No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC312050.46075763882418724No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC310690.45874952990317813No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT295510.4363354906230911No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT269400.3977827524410705No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA254000.3750438720119967No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT235840.3482297117138161No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT235490.34771291897679174No Hit
TGCTGCGTTACTTACCACGAATTGCAGACGCTTAGAGTGGTGAAATTTCG186240.2749927981240719No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA184690.2727041445743924No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG183100.2703564289976244No Hit
TACTCCGGAGTTAACAGAAAATCATACCTAATCTAGTACTTATAGTAAGG154950.2287915274340901No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA145180.21436562731772316No Hit
AAGAGAGGAGAAGGCTTAGTAGTTTAGACTGTTCTTCTATTAATTAATCT143350.21166353957842413No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT137660.20326196622508452No Hit
CAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGC127900.18885083161548966No Hit
GGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTA123660.1825902567441083No Hit
TAGTACCTGGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTA116870.17256447764583485No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA110110.16258299506787777No Hit
GGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTAAACTGAAA109310.16140175452610772No Hit
TACCACGAATTGCAGACGCTTAGAGTGGTGAAATTTCGAACGCATAGCAC108510.16022051398433765No Hit
AGTACTTATAGTAAGGTAAGTTTTACATCGATGTTGTATTCAGATAATCT106240.15686874394706507No Hit
AGTACCTGGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTAA104980.1550082900937772No Hit
CAGCCGGAATGTTAAGATCTTTAGTAATATTTTTTAAATTTGGACTATAC101050.14920544593233176No Hit
GACGTGATATTAGTTTAATTCATTGTGAGATAGAATTGTTTATCTTGATA100950.1490577908646105No Hit
GGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAA99610.14707921295714563No Hit
ACTCCGGAGTTAACAGAAAATCATACCTAATCTAGTACTTATAGTAAGGT99130.14637046863208358No Hit
ATGGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTT97420.14384556697405007No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG94040.13885482568507151No Hit
CTATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATT93510.13807225382614885No Hit
AGCTTCAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTT80910.11946771529327029No Hit
TGTACTTTAATTGATGTTAATTATGAAATTATTATATTTTCTTTTAGATC79370.1171938272503629No Hit
GGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTGTT79240.11700187566232527No Hit
TCCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGA77800.11487564268713915No Hit
TTCAGGGAATTAAAATTTGATCATGGTTTAAGATGATTTAAAATGGTATT74770.110401694135185No Hit
AAATGGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCT72920.10767007538234172No Hit
ACCTCGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTA67830.10015443243532966No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)