FastQCFastQC Report
Tue 27 Jun 2023
SRR1041223.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041223.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35664681
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG19570635.4873980227104795No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT1873300.5252535414518358No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT1734220.4862569778767964No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT1003770.28144651006411636No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA956320.2681420310474668No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC941960.2641156386622384No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC890050.24956062273485635No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT855360.239833912996446No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA851560.23876843311734655No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT687410.19274250623466951No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA665240.18652627230844993No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG609370.17086091419126953No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA601130.16855050519027492No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT565680.15861069947604467No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG508010.14244064036350138No Hit
CACTTGTGAGTCTTCAACGAATTCAACAATGTCCGGACGTGGAAAGGGAG459160.12874361612823623No Hit
CAGCCGGAATGTTAAGATCTTTAGTAATATTTTTTAAATTTGGACTATAC449710.12609393590258103No Hit
GTCAGCGCGATATGCTCAAGTAAAAGATTAAGCCATGCATGCTTTGATTC413110.11583168233020225No Hit
CTATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATT410910.11521482555809205No Hit
TTCAGGGAATTAAAATTTGATCATGGTTTAAGATGATTTAAAATGGTATT402070.11273618289197652No Hit
GGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTAAACTGAAA361700.10141686112375434No Hit
ACCTCGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTA358430.1004999876488451No Hit
CAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGC357660.10028408777860652No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)