FastQCFastQC Report
Tue 27 Jun 2023
SRR1041225.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041225.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31030773
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTAGAATTTGGATTTATATTAGTGAATTTTCATAATTTTAAGATTTG15508344.997729189666013No Hit
CGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACT1495440.48192160730253164No Hit
CGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATT1161240.3742220665917668No Hit
TGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCC924150.29781726675000975No Hit
TAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTC745000.24008425442704892No Hit
AGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTAT737120.23754483976277355No Hit
TTGTGATAACTGTTTTTATTTTTATATTAGAAAATTATATATTATATAAT648240.20890230481851033No Hit
ATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTA576890.18590900072002717No Hit
CCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAA498450.1606308679451846No Hit
GGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAA451730.14557484597628295No Hit
CAGCCGGAATGTTAAGATCTTTAGTAATATTTTTTAAATTTGGACTATAC439690.1416948266161465No Hit
AGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTTCCAGTATG431860.139171524989081No Hit
GCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTAT414410.13354807500283672No Hit
CAGCGATGGATCGGTTGCATCGAGTATCGATGAAGAACGCAGCTTGCTGC381010.12278456614664417No Hit
AGGGCAGAATATTATATAATAGTATTTCACTATATTTAATTTAAAGAATT373100.12023548366004289No Hit
TTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTG361570.11651981727944709No Hit
GTGAGGGCGAAACCGCGAATGGCTCATTAAATCAGTCATAATCTACTTGA344000.11085769600389911No Hit
TGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGA315280.10160236743055032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)